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Human Brunello kinome pooled library, guides 1-4 in lentiCRISPRv2 backbone

Pooled Library

#75314

Purpose

Human sgRNA library in backbone lentiCRISPRv2 targeting 763 kinase genes and containing 3,052 unique sgRNAs along with 100 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CRISPR-Puro

Plasmid

#208646

Purpose

pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.

Depositor

Quanfu Ma

Type

Empty backbone

Use

CRISPR

Tags

Clawed frog nucleoplasmin NLS, Flag, Puromycin re…

Expression

Mammalian

Promoter

CMV and U6 in tandem

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-psRaf1

Plasmid

#89364

Purpose

A lenti-viral vector expressing photoswitchable Raf1 in mammalian cells

Depositor

Michael Lin

Article

Zhou et al Science. 2017 Feb 24;355(6327):836-842.

Insert

psRaf1 (RAF1 Human, Synthetic)

Use

Lentiviral

Tags

Human influenza hemagglutinin (HA) tag and pdDron…

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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Human Brunello kinome pooled library, guides 1-4 in lentiGuide-Puro backbone

Pooled Library

#75312

Purpose

Human sgRNA library in backbone lentiGuide-Puro targeting 763 kinase genes and containing 3,052 unique sgRNAs along with 100 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pLL3.7m-psMEK2

Plasmid

#89363

Purpose

A lenti-viral vector expressing photoswitchable MEK2 in mammalian cells

Depositor

Michael Lin

Article

Zhou et al Science. 2017 Feb 24;355(6327):836-842.

Insert

psMEK2 (MAP2K2 Human, Synthetic)

Use

Lentiviral

Tags

Human influenza hemagglutinin (HA) tag and pdDron…

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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Human lentiviral CRISPR library_v1

Pooled Library

#69763

Purpose

Used for genome scale lentiviral CRISPR screening. Target sites were chosen in a region close to the translation initiation site.

Depositor

Haoquan Wu

Article

Ma et al Cell Rep. 2015 Jul 28;12(4):673-83. doi:

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
pLL3.7m-psCDK5

Plasmid

#89365

Purpose

A lenti-viral vector expressing photoswitchable CDK5 in mammalian cells

Depositor

Michael Lin

Article

Zhou et al Science. 2017 Feb 24;355(6327):836-842.

Insert

psCDK5 (CDK5 Human, Synthetic)

Use

Lentiviral

Tags

Human influenza hemagglutinin (HA) tag and pdDron…

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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Xiaole Shirley Liu lab Human CRISPR lentiviral library

Pooled Library

#1000000132

Purpose

CRISPR gRNA knockout pooled library for targeting the human genome

Depositor

Xiaole Shirley Liu

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only
pLV-hU6-ZNF865-hUbC-dCas9-KRAB-eGFP

Plasmid

#241308

Purpose

3rd gen lenti vector encoding dCas9 (s. Pyogenes) fused with the KRAB and a 2A eGFP fluorescence marker. Includes guide for suppression of the ZNF865 gene

Depositor

Robby Bowles

Insert

ZNF865 sgRNA (ZNF865 Human)

Use

Lentiviral

Available Since

Dec. 8, 2025

Availability

Academic Institutions and Nonprofits only

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[pSK468] pLJC5 Flag-Depdc5

Plasmid

#109337

Purpose

Lenti-viral expression of Flag-tagged wildtype Depdc5

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

FLAG

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pSMAL-CellTag-V2 (Concentrated Lentiviral Prep)

Viral Prep

#115644-LVC

Purpose

Ready-to-use Concentrated Lentiviral Prep particles produced from pSMAL-CellTag-V2 (#115644). In addition to the viral particles, you will also receive purified pSMAL-CellTag-V2 plasmid DNA. Ready-to-use lentiviral particles carrying version 2 of the CellTag barcoding library to combinatorially index cells for single-cell analysis of clonal dynamics.

Depositor

Samantha Morris

Article

Biddy et al Nature. 2018 Dec;564(7735):219-224. doi:

Promoter

Minimal CMV

Tags

EGFP

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only
[pSK467] pLJC5 HA-Depdc5

Plasmid

#109343

Purpose

Lenti-viral expression of HA-tagged wildtype Depdc5

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

HA

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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lenti.DTR.GFP

Plasmid

#201962

Purpose

Lentiviral vector that expresses diptheria toxin receptor (DTR) C-terminally fused to EGFP

Depositor

Bernhard Schmierer

Article

Fernandez Moro et al Nat Commun. 2023 Aug 18;14(1):5024. doi:

Insert

Diptheria Toxin Receptor

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1alpha

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti_MS2_P65_HSF1_2A_puro

Plasmid

#118699

Purpose

To express MS2-P65-HSF1

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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Liu Mouse CRISPR Knockout Library in lentiCRISPRv2-blast

Pooled Library

#1000000173

Depositor

Xiaole Shirley Liu

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only
pKC148_Lenti_Tetoff_MVP_EF1a_tagRFP

Plasmid

#250949

Purpose

A lentiviral vector express MVP gene under a Tetoff system. Contains tagRFP as selection marker.

Depositor

Fei Chen

Article

Chao et al Science. 2026 Jan 15:eadz9353. doi: 10.1

Insert

MVP (MVP Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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HK_13_Bla_Lenti_Kir2.1

Plasmid

#136632

Purpose

encodes Kir2.1 potassium channel for lentiviral transduction

Depositor

Adam Cohen

Article

McNamara et al Nat Phys. 2020 Mar;16(3):357-364. doi: 1

Insert

Kir2.1

Use

Lentiviral

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti.pFOXA2.5'.GFP

Plasmid

#59407

Purpose

expresses eGFP from an about 1350 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during development

Depositor

Kai-Christian Sonntag

Article

Aguila et al Stem Cells Transl Med. 2014 Jul 14. pii:

Insert

approx. 1350 bp FOXA2 promoter, eGFP (FOXA2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

FOXA2

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pKC152_Lenti_tetoff_PABP-INT_EF1a_EGFP

Plasmid

#250950

Purpose

A lentiviral vector express PABP-INT fusion protein under a Tetoff system. Contains EGFP as selection marker.

Depositor

Fei Chen

Article

Chao et al Science. 2026 Jan 15:eadz9353. doi: 10.1

Insert

PABPC1 (PABPC1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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Lenti_sgRNA_MS2_neo

Plasmid

#118650

Purpose

To clone sgRNA for activation dCas9

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Type

Empty backbone

Use

Lentiviral

Promoter

U6

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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