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pAO122 (Apr LEU2 MRC1)

Plasmid

#41923

Depositor

Stephen Elledge

Publication

Osborn et al Genes Dev. 2003 Jul 15;17(14):1755-67.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Mediator of the Replication Checkpoint

Available since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pJB94

Plasmid

#208752

Purpose

Theophylline-based control of repA on a C. difficile plasmid for use in allelic exchange

Depositor

Joseph Sorg

Publication

Brehm et al Anaerobe. 2024 Apr 29;88:102858. doi: 10

Insert

sacB (sacB )

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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mAIRN CD

Plasmid

#207412

Purpose

Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).

Depositor

Karlene Cimprich

Publication

Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

Insert

mAIRN

Expression

Bacterial and Mammalian

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-B2 (GB3374)

Plasmid

#160636

Purpose

pLX series: pBBR1-based T-DNA binary vector for plant transformation (alpha1)

Depositor

Juan Antonio García, Fabio Pasin

Publication

Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

Type

Empty backbone

Use

Synthetic Biology

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT PUR mGSK3AWT

Plasmid

#35159

Depositor

Jim Woodgett

Publication

Doble et al Dev Cell. 2007 Jun;12(6):957-71.

Insert

GSK-3α (Gsk3a Mouse)

Expression

Mammalian

Promoter

CMV

Available since

May 18, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
Pinco-mNrp1dSEA

Plasmid

#21935

Depositor

Guido Serini

Publication

Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

Insert

neuropilin 1 (Nrp1 Mouse)

Use

Retroviral

Tags

HA and His

Mutation

deleted amino acids 920-923

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
dCas13d-BFP

Plasmid

#215720

Purpose

Catalytically inactive dCasRx with 2A-BFP

Depositor

Chaolin Zhang

Publication

Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

Insert

dCasRx

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-hCaspase-8

Plasmid

#208786

Purpose

Inducibly expresses human Caspase 8 fused to light-activatable Cry2

Depositor

Petr Broz

Publication

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Caspase 8 (CASP8 Human)

Use

Lentiviral

Tags

Cry2 mCherry

Expression

Mammalian

Mutation

CARD domain is removed

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgBAP1-2

Plasmid

#125838

Purpose

KO BAP1 gene

Depositor

Boyi Gan

Publication

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLX317-QKI

Plasmid

#115441

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Publication

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

QKI (QKI Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc

Plasmid

#107982

Purpose

expression of OPN splice variant c

Depositor

Georg Weber

Publication

He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

Insert

osteopontin splice variant c (SPP1 Human)

Expression

Mammalian

Mutation

splice variant

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNb

Plasmid

#107981

Purpose

expression of OPN splice variant b

Depositor

Georg Weber

Publication

He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

Insert

osteopontin splice variant b (SPP1 Human)

Expression

Mammalian

Mutation

splice variant

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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PZac2.1 hSyn1-BioID2-BioID2-HA

Plasmid

#176855

Purpose

Expresses the promiscuous small biotin ligase, BioID2, in neurons

Depositor

Baljit Khakh

Publication

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

hSyn1

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSCARS1

Plasmid

#174868

Purpose

Contains TEF1p-TEF1in-hGFP-CYCt cassette and wildtype ARS1

Depositor

Zengyi Shao

Publication

Lopez et al Appl Microbiol Biotechnol. 2021 Aug 6. p

Insert

ARS1

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-actin

Plasmid

#118378

Purpose

Gateway compatible donor vector with WT actin.

Depositor

Maria Vartiainen

Publication

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Available since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRIM3-rfp

Plasmid

#74073

Purpose

GentR version of pRIM2 carrying rfp

Depositor

Timothy Lu

Publication

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

rfp

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

ptac

Available since

June 13, 2016

Availability

Academic Institutions and Nonprofits only

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hHpr1-GST

Plasmid

#11200

Depositor

Robin Reed

Publication

Masuda et al Genes Dev. 2005 Jul 1. 19(13):1512-7.

Insert

Hpr1 (THOC1 Human)

Tags

GST

Expression

Bacterial

Available since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA-NES-ZapCY2

Plasmid

#59016

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Publication

Qin et al ACS Chem Biol. 2013 Nov 15;8(11):2366-71

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
GFP-BLM DM

Plasmid

#80071

Purpose

Expression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutations

Depositor

Nathan Ellis

Publication

Eladad et al Hum Mol Genet. 2005 May 15;14(10):1351-6

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BLM (BLM Human)

Tags

GFP-BLM

Expression

Mammalian

Mutation

K317R (AAA>AGA) and K331R (AAA>AGA)

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-N2-6MYC-hMID2

Plasmid

#51035

Purpose

mammalian expression of myc-tagged human MID2

Depositor

Timothy Cox

Publication

Short et al BMC Cell Biol. 2002;3:1. Epub 2002 Jan 4

Insert

MID2 (MID2 Human)

Tags

MYC

Expression

Mammalian

Promoter

CMV

Available since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

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