Addgene

We narrowed to 16,907 results for: pho

Showing: 1921 - 1940 of 16907 results

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995 pSG5L HA PTEN S370A

Plasmid

#10754

Depositor

William Sellers

Article

Vazquez et al Mol Cell Biol. 2000 Jul . 20(14):5010-8.

Insert

PTEN (PTEN Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S370A

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pET15b/GgVcl 884-1066 (aka Vt)

Plasmid

#46176

Depositor

Susan Craig

Article

Steimle et al J Biol Chem. 1999 Jun 25;274(26):18414-2

Insert

Vcl 884-1066 (VCL Chicken)

Use

Tags

His

Expression

Bacterial

Mutation

aa 884-1066 (tail domain)

Promoter

T7

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-p110α_wt

Plasmid

#39489

Depositor

Julian Downward

Article

Rodriguez-Viciana et al EMBO J. 1996 May 15;15(10):2442-51.

Insert

p110 (PIK3CA Bovine)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only
pPD2X

Plasmid

#86967

Purpose

Expresses PCNA2 protein fused phosphite dehydrogenase and putidaredoxin in E. coli

Depositor

Teruyuki Nagamune

Article

Watanabe et al ChemCatChem 2013 Dec;5(12):3835-3840

Insert

PCNA2 fused to phosphite dehydrogenase and putidaredoxin

Use

Tags

His6 tag

Expression

Bacterial

Mutation

E175A/A176R in PTDH, C73S/C85S in PdX

Promoter

T7 promoter

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pcA17/F527

Plasmid

#17684

Depositor

David Shalloway

Article

Yaciuk et al Mol Cell Biol. 1989 Jun . 9(6):2453-63.

Insert

c-src S17A Y527F (SRC Chicken)

Use

Tags

Expression

Mammalian

Mutation

Ser 17 changed to Ala. Tyr 527 changed to Phe .

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pRS414-59

Plasmid

#23087

Depositor

Sharon Dent

Article

Edmondson et al J Biol Chem. 2002 Aug 16. 277(33):29496-

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H3 changed Serine 10 to Alanine, H3 S10A.

Promoter

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
OSCA1.2-L438K-peGFP-N1

Plasmid

#232023

Purpose

Expresses OSCA1.2-L438K-peGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

OSCA1.2

Use

Tags

peGFP

Expression

Mammalian

Mutation

L438K

Promoter

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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27 pCR plcg 1 CDS

Plasmid

#13058

Depositor

Nathan Lawson

Article

Lawson et al Genes Dev. 2003 Jun 1. 17(11):1346-51.

Insert

plcg1 (plcg1 Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pJD112

Plasmid

#23088

Depositor

Sharon Dent

Article

Edmondson et al J Biol Chem. 2002 Aug 16. 277(33):29496-

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone 3 Changed Serine 10 to Aspartate, H3 S10D.

Promoter

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pJD113

Plasmid

#23089

Depositor

Sharon Dent

Article

Edmondson et al J Biol Chem. 2002 Aug 16. 277(33):29496-

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H3 changed Serine 10 to Glutamamte, H3 S1…

Promoter

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pcAla12/Ala17

Plasmid

#17682

Depositor

David Shalloway

Article

Yaciuk et al Mol Cell Biol. 1989 Jun . 9(6):2453-63.

Insert

c-src S12A S17A (SRC Chicken)

Use

Tags

Expression

Mammalian

Mutation

Ser 12 changed to Ala. Ser 17 changed to Ala.

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pcA12/F527

Plasmid

#17683

Depositor

David Shalloway

Article

Yaciuk et al Mol Cell Biol. 1989 Jun . 9(6):2453-63.

Insert

c-src S12A Y527F (SRC Chicken)

Use

Tags

Expression

Mammalian

Mutation

Ser 12 changed to Ala. Tyr 527 changed to Phe .

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pCAG-FLAG-puro-PTPIP51(1-470)

Plasmid

#170536

Purpose

Expresses PTPIP51(1-470) in human HeLa cells after transfection

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag Jnk1a1(apf)

Plasmid

#13846

Depositor

Roger Davis

Article

Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

Insert

Jnk1a1(apf) (MAPK8 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

replaced dual phosporylation motif Thr(183)-Pro-T…

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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INPP5D-eGFP D673G mutant

Plasmid

#161999

Purpose

Mammalian expression vector, containing a GFP tagged phosphatase dead version of INPP5D (D673G)

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

INPP5D (INPP5D Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

D673G

Promoter

Available since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-INPP4B C842A mutant

Plasmid

#161996

Purpose

Mammalian expression vector containing a phosphatase dead mutant of INPP4B (C842A). Also contains mCherry tag

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

INPP4B (INPP4B Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

C842A

Promoter

Available since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-baPrs-hdNadV

Plasmid

#91950

Purpose

pET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyi

Depositor

George Cătălin Marinescu

Article

Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

Inserts

Putative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
Phosphoribosyl Pyrophosphate Synthetases

Use

Tags

Expression

Bacterial

Mutation

changed Leucine 135 to Isoleucine

Promoter

T7

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS_m-AKAR5’

Plasmid

#114140

Purpose

Mammalian expression of the AKAR5’ sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

AKAR5' C-terminally tagged by KRas C tail

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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siRNA resistant ABHD16A-S176AS306A-mNG

Plasmid

#195165

Purpose

mammalian expression of siRNA-resistant mutant ABHD16A tagged with mNeonGreen

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

ABHD16A (ABHD16A Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Wobble mutations for siRNA resistance from L90 to…

Promoter

CMV

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant ABHD16A-H189AD194A-mNG

Plasmid

#195166

Purpose

mammalian expression of siRNA-resistant mutant ABHD16A tagged with mNeonGreen

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

ABHD16A (ABHD16A Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Wobble mutations for siRNA resistance from L90 to…

Promoter

CMV

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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