We narrowed to 4,776 results for: Drosophila

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pCRISPaint-T2A-Cas9-T2A-GFP-3xP3-RFP

Plasmid

#127560

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Cas9-T2A-GFP-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

Cas9-T2A-GFP

Use

CRISPR

Expression

Insect

Mutation

EGFP-D102G see depositor comments below

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1

Plasmid

#22420

Depositor

Elisa Izaurralde

Article

Rehwinkel et al RNA. 2005 Nov . 11(11):1640-7.

Insert

Argonaut-1, variant A (AGO1 Fly)

Use

Drosophila expression

Tags

lambdaN-HA

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pET28b Me31B-3Myc

Plasmid

#113001

Purpose

For protein expression and purification of Myc-tagged full-length Drosophila me31B

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length me31B (me31B Fly)

Tags

Myc

Expression

Bacterial

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pUAS-CFP-MEK

Plasmid

#42077

Depositor

Norbert Perrimon

Article

Friedman et al Nature. 2006 Nov 9;444(7116):230-4. Epub

Insert

Drosophila MEK ortholog (Dsor1 Fly)

Tags

CFP

Expression

Insect

Promoter

Hsp70

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
pGex4t2 with full-length SUUR

Plasmid

#112990

Purpose

Expresses fusion of GST and full-length Drosophila SUUR

Depositor

Terry Orr-Weaver

Article

Nordman et al Cell Rep. 2014 Nov 6;9(3):841-9. doi: 10

Insert

SuUR (SuUR Fly)

Expression

Bacterial

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Laccase2 MCS-ZKSCAN1 548-1047

Plasmid

#69886

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA in Drosophila

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (stw, ZKSCAN1 Human)

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAC-U63-gRNA2.1-tub-miRFP680-T2A-HO1(HA)

Plasmid

#250795

Purpose

Drosophila transgenic vector: nMAGIC vector with tub promoter, 3x HA tags, miRFP680, and HO1. Optimized nMAGIC vector with a far red/infra red FP marker.

Depositor

Chun Han

Article

Shen et al Elife. 2026 Mar 11;14:RP108453. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

Feb. 18, 2026

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast CG1358

Plasmid

#218541

Purpose

Drosophila CG1358 cDNA (human codon optimized)

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

CG1358 (HisT Fly)

Use

Retroviral

Expression

Mammalian

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[R280A,K284A]

Plasmid

#188598

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

R280A [CGA=>GCA], K284A [AAG=> GCG]

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[C399-625 CM]

Plasmid

#188588

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler C-terminal domain, catalytic mutant

Tags

FLAG-Myc

Expression

Insect

Mutation

D435A [GAT=>GCC]; E435A [GAA=>GCC]

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[C399-625]-Gwpep(Mm)

Plasmid

#188589

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

GW-peptide (mouse) C-terminally tagged C-terminal domain of Nibbler

Tags

FLAG-Myc and GWpep mm

Expression

Insect

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Gwpep(Mm)-Nbr[C399-625]

Plasmid

#188591

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

GW-peptide (mouse) N-terminally tagged C-terminal domain of Nibbler

Tags

FLAG-Myc-Gwpep mm

Expression

Insect

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[R272A,K276A]

Plasmid

#188596

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

R272A [CGG=> GCG], K276A [AAG=>GCG]

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pBABR mCherry-Bcd-LEXY

Plasmid

#182596

Purpose

Drosophila integration plasmid expressing mCherry-Bcd-LEXY fusion protein under the maternal tubulin promoter.

Depositor

Jared Toettcher

Article

Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

Insert

Bcd (bcd Fly)

Tags

LEXY and mCherry

Expression

Insect

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Staufen-dsRBD(+6)

Plasmid

#171546

Purpose

Encoding part of the Drosophila Staufen protein from M571 to P656

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Staufen (stau Fly)

Expression

Bacterial

Mutation

a six amino acid_TRLTEG_insertion in the L1 region

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1 BICC FL

Plasmid

#112998

Purpose

For protein expression and purification of full-length Drosophila BicC

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length BicC (BicC Fly)

Expression

Bacterial

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Laccase2 MCS-ZKSCAN1 548-847

Plasmid

#69885

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA in Drosophila

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (stw, ZKSCAN1 Human)

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pUAS-hhN-HA

Plasmid

#240236

Purpose

Expression of hhN-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Insert

hedgehog N-terminal fragment (hh Fly)

Tags

HA-spGFP11

Expression

Insect

Mutation

aa 1-257

Promoter

UAS

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pnCS_DSep2_Peanut-TEV-Strep

Plasmid

#174495

Purpose

bacterial co-expression of Drosophila Sep2 and of Drosophila Peanut

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Inserts

Sep2 (Sep2 Fly)
peanut (pnut Fly)

Tags

TEV-Strep

Expression

Bacterial

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pJFRC28-10XUAS-IVS-GFP-p10

Plasmid

#36431

Depositor

Gerald Rubin

Article

Pfeiffer et al Proc Natl Acad Sci U S A. 2012 Apr 9.

Insert

GFP

Expression

Insect

Available Since

May 3, 2012

Availability

Academic Institutions and Nonprofits only

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