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Showing: 1941 - 1960 of 2379 results

  1. CAG sHRPa-FRB-pre mGRASP

    Plasmid
    #73145
    Purpose
    Large fragment of split HRP and FRB fused to the extracellular terminus of the "pre mGRASP" scaffold published by Magee, in which the extracellular domain of NRX is replaced by portions of CD4.
    Depositor
    Alice Ting
    Article
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Insert
    sHRPa-FRB-pre mGRASP
    Use
    Tags
    Flag tag
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73145-plasmid-map-sequence-id-134517

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  2. pET19b-EcMscL-TEV-mEGFP

    Plasmid
    #165097
    Purpose
    Expresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.
    Depositor
    Neha Kamat
    Article
    Jacobs et al Proc Natl Acad Sci U S A. 2019 Feb 13. p
    Insert
    E. Coli Mechanosensitive Channel of Large Conductance (mscL E. coli)
    Use
    Synthetic Biology
    Tags
    monomeric Enhanced Green Fluorescent Protein
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    165097-plasmid-map-sequence-id-324525

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  6. pACEC3GH (Apr)

    Plasmid
    #110085
    Purpose
    Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Use
    Tags
    3C cleavage site - GFP - His10 Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110085-plasmid-map-sequence-id-211188

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  11. MinLibCas9

    Pooled Library
    #164896
    Purpose
    Human genome-wide CRISPR-Cas9 gRNA library, optimised by mining existing large-scale screening datasets, which provides a >60% reduction in size while preserving key information content
    Depositor
    Mathew Garnett
    Article
    Gonçalves et al Genome Biol. 2021 Jan 21;22(1):40. doi:
    Expression
    Mammalian
    Species
    Homo sapiens
    Use
    CRISPR and Lentiviral
    Available since
    May 20, 2021
    Availability
    Academic Institutions and Nonprofits only

  12. pDG459

    Plasmid
    #100901
    Purpose
    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100901-plasmid-map-sequence-id-197197

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  13. pDG458

    Plasmid
    #100900
    Purpose
    SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100900-plasmid-map-sequence-id-457690

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  14. pAAV MP-84 mRFP

    Plasmid
    #206926
    Purpose
    The micro promoter MP-84 is an AAV specific strong promoter for gene expression in mammalian cells. The small size of the promoter (84 bp) allows delivery of large genes in AAV.
    Depositor
    Markus Grompe
    Article
    Chai et al Mol Ther Methods Clin Dev. 2023 May 17;2
    Insert
    mRFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MP-84
    Available since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    206926-plasmid-map-sequence-id-407770

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  15. pAAV MP-135 mRFP

    Plasmid
    #206927
    Purpose
    The micro promoter MP-135 is an AAV specific strong promoter for gene expression in mammalian cells. The small size of the promoter (135 bp) allows delivery of large genes in AAV.
    Depositor
    Markus Grompe
    Article
    Chai et al Mol Ther Methods Clin Dev. 2023 May 17;2
    Insert
    mRFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MP-135
    Available since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    206927-plasmid-map-sequence-id-407771

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  17. Synapsin sHRPa-NRX in FSW lentiviral vector

    Plasmid
    #73147
    Purpose
    Large fragment of split HRP fused to the extracellular terminus of neurexin3B. This is a lentiviral plasmid, but can also be used for transfections. Synapsin promoter.
    Depositor
    Alice Ting
    Article
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Insert
    sHRPa-NRX
    Use
    Lentiviral
    Tags
    2x HA tag and Flag tag
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    June 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73147-plasmid-map-sequence-id-134524

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  18. pcDNA3.2-DEST-MatryoshCaMP6s

    Plasmid
    #100025
    Purpose
    Mammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    MatryoshCaMP6s
    Use
    Tags
    V5 Epitope Tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100025-plasmid-map-sequence-id-194289

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  20. pDG330

    Plasmid
    #100898
    Purpose
    SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100898-plasmid-map-sequence-id-195326

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Showing: 1941 - 1960 of 2379 results