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pAAV-TetON-Bmpr1A-ca

Plasmid

#217536

Purpose

AAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged constitutively-active BMPR1A receptor

Depositor

Peter Scheiffele

Article

Okur et al bioRxiv 2023

Insert

constitutively active BMP receptor 1a (BMPR1A Human)

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

Tet operon (TetO)

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-sgAAVS1-HepmTurquoise2

Plasmid

#192828

Purpose

Expresses sgRNA targeting AAVS1 (non-targeting control sgRNA for mouse) and mTurquoise2 from hepatocyte-specific promoter

Depositor

Kristin Knouse

Article

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Insert

mTurquoise2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 for sgRNA and hepatocyte-specific promoter (HS…

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-cytoFLARE1.0-TEV

Plasmid

#234517

Purpose

Transcriptional reporter for detecting calcium transients in neuronal cultures (TEVp)

Depositor

Wenjing Wang

Article

Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

Insert

EGFP-P2A-CaM-V5-TEVp

Use

AAV

Expression

Mammalian

Available Since

April 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV2-mtIF3 shRNA_TagRFP657

Plasmid

#182367

Purpose

shRNA targeting mtIF3 mRNA

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Insert

mtIF3 (Mtif3 Mouse)

Use

AAV and RNAi

Expression

Mammalian

Promoter

U6 promoter

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV CAG Barcode6

Plasmid

#229068

Purpose

Expression mapping

Depositor

Scott Sternson

Insert

CAG Barcode6

Use

AAV

Available Since

Dec. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV CAG Barcode4

Plasmid

#229065

Purpose

Expression mapping

Depositor

Scott Sternson

Insert

CAG Barcode4

Use

AAV

Available Since

Dec. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-sgAAVS1-HepmTurquoise2

Plasmid

#192828

Purpose

Expresses sgRNA targeting AAVS1 (non-targeting control sgRNA for mouse) and mTurquoise2 from hepatocyte-specific promoter

Depositor

Kristin Knouse

Article

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Insert

mTurquoise2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 for sgRNA and hepatocyte-specific promoter (HS…

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-SomaGCaMP6f2

Plasmid

#158756

Purpose

Cell body-targeted GCaMP6f, under synapsin promoter: GCaMP6f followed by a linker, and the EE-RR coiled-coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

SomaGCaMP6f2

Use

AAV

Expression

Mammalian

Promoter

synapsin promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-WHaloCaMP1a

Plasmid

#205310

Purpose

Production of AAV particles encoding WHaloCaMP1a from the CaMKII promoter

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a

Use

AAV

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-DIO-TVA-2A-oG

Plasmid

#178431

Purpose

Expresses TVA and optimized Rabies G (oG) protein in a FLEX cassette

Depositor

Edward Callaway

Inserts

TVA950
Optimized G

Use

AAV and Cre/Lox; Adeno-associated virus

Promoter

hSyn

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CASI-Cas9C

Plasmid

#80932

Purpose

Expresses Cas9C in mammalian cells; derived from pZac2.1 with CASI promoter.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9C

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Sa-SauriCas9

Plasmid

#135967

Purpose

Expresses Sa-SauriCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-SomaGCaMP6f2

Plasmid

#158756

Purpose

Cell body-targeted GCaMP6f, under synapsin promoter: GCaMP6f followed by a linker, and the EE-RR coiled-coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

SomaGCaMP6f2

Use

AAV

Expression

Mammalian

Promoter

synapsin promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-cytoFLARE1.0-TEV

Plasmid

#234517

Purpose

Transcriptional reporter for detecting calcium transients in neuronal cultures (TEVp)

Depositor

Wenjing Wang

Article

Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

Insert

EGFP-P2A-CaM-V5-TEVp

Use

AAV

Expression

Mammalian

Available Since

April 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pAAV-CaMKII-WHaloCaMP1a

Plasmid

#205310

Purpose

Production of AAV particles encoding WHaloCaMP1a from the CaMKII promoter

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a

Use

AAV

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pDV24_AAV2_DART-VADAR_iRFP720-sensor

Plasmid

#200411

Purpose

AAV vector encoding a DART-VADAR sensor for iRFP720 mRNA

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

DART VADAR sensor

Use

AAV and Synthetic Biology

Tags

MCP-ADAR2dd(E488Q)-NES, TagBFP, and mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV CAG Barcode6

Plasmid

#229068

Purpose

Expression mapping

Depositor

Scott Sternson

Insert

CAG Barcode6

Use

AAV

Available Since

Dec. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV CAG Barcode4

Plasmid

#229065

Purpose

Expression mapping

Depositor

Scott Sternson

Insert

CAG Barcode4

Use

AAV

Available Since

Dec. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-DIO-TVA-2A-oG

Plasmid

#178431

Purpose

Expresses TVA and optimized Rabies G (oG) protein in a FLEX cassette

Depositor

Edward Callaway

Inserts

TVA950
Optimized G

Use

AAV and Cre/Lox; Adeno-associated virus

Promoter

hSyn

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAG-FLEX.SF-iGluSnFR.A184S

Plasmid

#106186

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S

Promoter

CAG

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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