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BFP-GFP-bidirectional MS2 splicing reporter CORO1B intron

Plasmid

#235086

Purpose

GFP splicing reporter with CORO1B intron

Depositor

Mitchell Guttman

Publication

Bhat et al Nature. 2024 May;629(8014):1165-1173. do

Insert

BFP-GFP-bidirectional MS2 splicing reporter CORO1B intron (CORO1B Human)

Use

AAV

Expression

Mammalian

Promoter

custom bidirectional

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pStreptagII 3C EGFR EABR

Plasmid

#234994

Purpose

For production of Extracellular Vesicles (EVs), with Epithelial Growth Factor Receptor on their surface, Strep-tag II labeled on its N-terminus

Depositor

Monica Gonzalez-Magaldi, Daniel Leahy

Publication

Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

Insert

EGFR-EABR (EGFR Human)

Tags

Streptag-II, HRV 3C site

Expression

Mammalian

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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mCh-KIF5A*-strep

Plasmid

#120163

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF5A-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Publication

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 5A (Kif5a Mouse)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF5A: 1-559 aa

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PEX-mCherry-CRY2

Plasmid

#102250

Purpose

Expresses fusion of CRY2PHR with mCherry and PEX3 (1-42)

Depositor

Bianxiao Cui

Publication

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

PEX-mCherry-CRY2 (PEX3, CRY2 Mustard Weed, Human)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pJJS011

Plasmid

#188334

Purpose

PCR template for repair templates to tag a gene with a mTagBFP2 and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Publication

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

mIAA7::mTagBFP2::Linker

Expression

Bacterial

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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TC646

Plasmid

#164892

Purpose

Pdpy30::loxP::mcherry::let-858terminator::loxP::GFP

Depositor

Tian Chi

Publication

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

GFP

Expression

Worm

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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TC872

Plasmid

#164895

Purpose

TRE::nCre, inducible nCre expression when co-express with rtTA

Depositor

Tian Chi

Publication

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

nCre

Expression

Worm

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGC235

Plasmid

#19683

Depositor

Jane Hubbard

Publication

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Plim-7

Use

Gateway Cloning

Available since

March 3, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCM5.66

Plasmid

#19370

Depositor

Geraldine Seydoux

Publication

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

pos-1 3'utr in pDONRP2RP3

Expression

Worm

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pCM5.60

Plasmid

#19374

Depositor

Geraldine Seydoux

Publication

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

fog-1 3'utr in pDONRP2RP3

Expression

Worm

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pEY52

Plasmid

#191055

Purpose

odr-1 fluorescent neural reporter driving nuclear mNeptune2.5 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

odr-1 (odr-1 Nematode)

Expression

Worm

Mutation

None

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCZGY405

Plasmid

#172694

Purpose

Punc-25-mcherry::rab-3 Expresses Red-tagged synapses specifically in C. elegans GABAergic motor neurons

Depositor

Yishi Jin

Publication

Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

Insert

rab-3 (rab-3 Nematode)

Tags

Punc-25 and mcherry

Expression

Worm

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCM1.26

Plasmid

#21377

Depositor

Geraldine Seydoux

Publication

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

L3787 promoter::GFP in pDONRP4P1R

Use

Gateway Cloning

Available since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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pGC157

Plasmid

#19682

Depositor

Jane Hubbard

Publication

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Prpl-28

Use

Entry clone

Expression

Worm

Available since

March 3, 2009

Availability

Academic Institutions and Nonprofits only

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pAS1-rab-3P::his-24::CyOFP

Plasmid

#124347

Purpose

Nuclear Localized Pan-Neuronal CyOFP

Depositor

Andrew Leifer

Insert

rab-3, CyOFP (rab-3 Nematode)

Expression

Worm

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAS1-rab3P::his-24::mNeptune

Plasmid

#124350

Purpose

Nuclear Localized Pan-Neuronal mNeptune

Depositor

Andrew Leifer

Insert

rab-3, mNeptune (rab-3 Nematode)

Expression

Worm

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pEY12

Plasmid

#191018

Purpose

Fluorescent reporter driving panneuronal nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

rab-3 (rab-3 Nematode)

Expression

Worm

Mutation

None

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEY51

Plasmid

#191054

Purpose

mec-3 fluorescent neural reporter driving nuclear CyOFP1 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

mec-3 (mec-3 Nematode)

Expression

Worm

Mutation

None

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBN281

Plasmid

#86726

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Publication

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Plin-31::FLP D5

Expression

Worm

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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oma-1 ORF in L4440

Plasmid

#82547

Purpose

oma-1 RNAi feeding construct

Depositor

Sean Ryder

Publication

Kaymak et al J Biol Chem. 2013 Oct 18;288(42):30463-7

Insert

oma-1 ORF

Expression

Worm

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only