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SypHer mt

Plasmid

#48251

Purpose

pH sensor, targeted to mitochondria

Depositor

Nicolas Demaurex

Article

Poburko et al J Biol Chem. 2011 Apr 1;286(13):11672-84

Insert

SypHer

Tags

2 mitochondrial targeting sequences

Expression

Mammalian

Mutation

C199S

Promoter

CMV

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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optoFcR

Plasmid

#224291

Purpose

Expression of an optogenetic Fc Receptor

Depositor

Meghan Morrissey

Article

Bond et al Dev Cell. 2024 Aug 9:S1534-5807(24)00457

Insert

Fc receptor gamma (Fcer1g Mouse)

Use

Lentiviral

Tags

mScarlet-Cry2-olig and myristoylation sequence

Mutation

aa 45-86 only

Promoter

SFFV

Available Since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2DY-EF

Plasmid

#221716

Purpose

Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2DY-EF (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

D651E and Y680F in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223-BMRF2

Plasmid

#195803

Purpose

Gateway Entry Clone of Epstein-Barr virus BMRF2

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BMRF2 (BMRF2 Epstein-Barr virus)

Use

Gateway Cloning

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BKRF2

Plasmid

#195773

Purpose

Gateway Entry Clone of Epstein-Barr virus BKRF2

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BKRF2 (BKRF2 Epstein-Barr virus)

Use

Gateway Cloning

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1 mito-mEmerald

Plasmid

#174542

Purpose

labels mitochondria with mEmerald

Depositor

Minna Roh-Johnson

Article

Kidwell et al eLife. 2023 Mar 6;12:e85494. doi: 10.755

Insert

MTS-mEmerald

Use

Lentiviral

Tags

mEmerald

Expression

Mammalian

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pH6-MBP-TEV-BirA

Plasmid

#179694

Purpose

Expresses His6 tagged MBP-BirA fusion protein

Depositor

Mark Arbing

Insert

BirA

Tags

His6-MBP

Expression

Bacterial

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-TagRFP-PuroR

Plasmid

#80971

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

TagRFP

Use

Gene tagging

Promoter

none

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pAW014-2

Plasmid

#85612

Purpose

Multi-gRNA delivery vector containing ugtP-gRNA.P395T for Bacillus subtilis

Depositor

Perry Chou

Article

Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

Insert

ugtP-gRNA.P395T

Expression

Bacterial

Available Since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR223-BFRF1

Plasmid

#195801

Purpose

Gateway Entry Clone of Epstein-Barr virus BFRF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BFRF1 (BFRF1 Epstein-Barr virus)

Use

Gateway Cloning

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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