We narrowed to 60,524 results for: Gene

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LentiCRISPRv2-bleo-COX1-4

Plasmid

#184722

Purpose

COX1-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog COX1 gene.

Use

CRISPR and Lentiviral

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-hyg2-COX2-4

Plasmid

#184723

Purpose

COX2-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog COX2 gene.

Use

CRISPR and Lentiviral

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-bleo-PTGER2

Plasmid

#184724

Purpose

EP2-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog EP2 gene.

Use

CRISPR and Lentiviral

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-bleo-PLA2G4A_3

Plasmid

#184725

Purpose

cPLA2-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog cPLA2 gene.

Use

CRISPR and Lentiviral

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-hyg2-PTGER4

Plasmid

#184726

Purpose

EP4-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog EP4 gene.

Use

CRISPR and Lentiviral

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-canis_PTGS1-3

Plasmid

#184727

Purpose

COX1-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog COX1 gene.

Use

CRISPR

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-canis_PTGS1-4

Plasmid

#184728

Purpose

COX1-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog COX1 gene.

Use

CRISPR

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-canis_PTGS2-3

Plasmid

#184729

Purpose

COX2-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog COX2 gene.

Use

CRISPR

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS1553 pLenti-U1a-SpCas9-miniU6-tracrRNA

Plasmid

#211819

Purpose

lentiviral vector expressing SpCas9 and tracrRNA for stable cell line extablishment

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

SpCas9, tracrRNA and blasticidin resistance gene

Use

Lentiviral

Expression

Mammalian

Promoter

U1a promoter and miniU6 promoter

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pL0_DE-sfGFP

Plasmid

#202156

Purpose

Level 0 plasmid containing a sfGFP fluorescent protein with DE overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

sfGFP gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_CD-sfGFP

Plasmid

#202147

Purpose

Level 0 plasmid containing a sfGFP fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

sfGFP gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-luciferase-reporter-CDK5RAP2-SVA

Plasmid

#202828

Purpose

pGL3 luciferase reporter with CDK5RAP2 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

intronic SVA within CDK5RAP2 gene

Use

Luciferase

Expression

Mammalian

Promoter

SV40

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-luciferase-reporter-AGPAT3-SVA

Plasmid

#202827

Purpose

pGL3 luciferase reporter with AGPAT3 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

intronic SVA located in AGPAT3 gene

Use

Luciferase

Expression

Mammalian

Promoter

SV40

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK-NP

Plasmid

#196607

Purpose

Dual-Luciferase Reporter vector carrying a 425bp-long Influenza virus NP sequence in forward direction

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

nucleocapsid protein (NP) gene, partial

Expression

Mammalian

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pENT-R4-bpFOG-L5

Plasmid

#186369

Purpose

Entry clone with ORF encoding Strong basal promoter of FOG flanked by Gateway recombination sequences

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

Basal promoter of the Friend Of Gata gene

Use

Protein expression

Promoter

T7 promoter

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2puro-Ptgfr

Plasmid

#170303

Purpose

A knock-out vector for the mouse Ptgfr

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A gRNA targeting the mouse Ptgfr gene.

Use

CRISPR and Lentiviral

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pNK108h

Plasmid

#156368

Purpose

Expresses heterlogous ribosomal proteins

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

S20, S16, S15 ribosomal genes

Use

Synthetic Biology

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pMST-1313

Plasmid

#118076

Purpose

pTEF1-cexA-tCYC1

Depositor

Michael Sauer

Article

Steiger et al Metab Eng. 2019 Mar;52:224-231. doi: 10.

Insert

pTEF1-cexA-tCYC1

Expression

Yeast

Promoter

pTEF1

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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csd2_121-308/pET21a(+)

Plasmid

#83293

Purpose

csd2, construct: 121-308, pET21a(+) no-tag

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Expression

Bacterial

Mutation

deleted amino acid 1-120

Available Since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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csd2_140-251/pET21a(+)

Plasmid

#83294

Purpose

csd2, construct:140-251, pET21a(+) C-tag

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acids 1-139 and 252-308

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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csd1_54-312/pET21a(+)

Plasmid

#83291

Purpose

csd1, construct: 54-312, pET21a(+) C-tag

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1543

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-53

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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csd2_121-308,S166A/pET28b(+)

Plasmid

#83301

Purpose

csd2, construct: 121-308, pET28b(+) NC-tag, mutation: S166A

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-120, and changed Serine 166 …

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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csd2_121-308,T151A/pET28b(+)

Plasmid

#83300

Purpose

csd2, construct: 121-308, pET28b(+) NC-tag, mutation: T151A

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-120, and changed Threonine 1…

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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csd2_121-308,Y197F/pET28b(+)

Plasmid

#83299

Purpose

csd2, construct: 121-308, pET28b(+) NC-tag, mutation: Y197F

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-120, and changed Tyrosine 19…

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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csd2_121-308,E165A/pET28b(+)

Plasmid

#83298

Purpose

csd2, construct: 121-308, pET28b(+) NC-tag, mutation: E165A

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-120, and changed Glutamic ac…

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
csd2_121-308/pET28b(+)

Plasmid

#83292

Purpose

csd2, construct: 121-308, pET28b(+) NC-tag

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-120

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
csd1_125-312/pET28b(+)

Plasmid

#83290

Purpose

csd1, construct: 125-312, pET28b(+) NC-tag

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1543

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-124

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFbx15(-73/+114)-Luc

Plasmid

#13405

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Upstream region of mouse Fbx15 gene

Use

Luciferase

Expression

Mammalian

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pFbx15(-201/+114)-Luc

Plasmid

#13406

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Upstream region of mouse Fbx15 gene

Use

Luciferase

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pFbx15(-332/+114)-Luc

Plasmid

#13407

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Upstream region of mouse Fbx15 gene

Use

Luciferase

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only