We narrowed to 9,664 results for: Coli

1. FB005

   Plasmid

   #119678

   Purpose

   Coding sequence nptII from E. coli domesticated into pUPD2 with AATG/GCTT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation

   Depositor

   Jose Marcos

   Article

   Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

   Insert

   geneticin resistance marker
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXylan

   Plasmid

   #36301

   Depositor

   Jay Keasling

   Article

   Bokinsky et al Proc Natl Acad Sci U S A. 2011 Dec 13;10

   Insert

   pXylan
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Two promoters from the E coli genome drive two di…

   Available since

   Nov. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAX1_HS∆P2

   Plasmid

   #225197

   Purpose

   Used to create a markerless deletion of the E. coli HS P2 phage

   Depositor

   Travis Wiles

   Article

   Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

   Insert

   P2 phage homology arms
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAX1_HS∆tum

   Plasmid

   #225198

   Purpose

   Used to create a markerless deletion of the E. coli HS tum gene

   Depositor

   Travis Wiles

   Article

   Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

   Insert

   tum homology arms
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAX1_HS_gpN:SpyTag

   Plasmid

   #225193

   Purpose

   Used for adding C-terminal SpyTag to the E. coli HS P2 phage major capsid protein

   Depositor

   Travis Wiles

   Article

   Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

   Insert

   gpN homology arms and SpyTag
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAX1_HS_gpN:SpyTag003

   Plasmid

   #225194

   Purpose

   Used for adding C-terminal SpyTag003 to the E. coli HS P2 phage major capsid protein

   Depositor

   Travis Wiles

   Article

   Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

   Insert

   gpN homology arms and SpyTag003
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSFDuet1-His-TEV-OTC_d1_Ub

   Plasmid

   #170729

   Purpose

   Scaffold design for studying ubiquitin interacting proteins by cryo-EM.

   Depositor

   Shang-Te Danny Hsu

   Article

   Chiu et al Biochemistry. 2021 Apr 13;60(14):1075-10

   Insert

   E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   The C-terminus of OTC was truncated by removing o…

   Promoter

   T7

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. EcRfaHKOW-L1L2NusG

   Plasmid

   #113126

   Purpose

   Expresses C-terminus of E. coli RfaH with L1 and L2 loops replaced with NusG residues

   Depositor

   James Berger

   Article

   Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

   Insert

   RfaH (rfaH Escherichia coli)
   

   Use

   Tags

   His6-MBP-N10-TEV

   Expression

   Bacterial

   Mutation

   Deleted residues 1-107, replaced L1 and L2 loops …

   Promoter

   T7

   Available since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. PatoM-OG241

   Plasmid

   #72842

   Purpose

   Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. OG241 backbone.

   Depositor

   Chris Barnes

   Article

   Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

   Insert

   PatoM
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PatoM

   Available since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGS1412

    Plasmid

    #63787

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCitrine Strep-tag II followed by URA3 selection marker

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    eCitrine-Strep-tag II followed by URA3 selection marker
    

    Use

    E. coli cloning vector

    Tags

    eCitrine-Strep-tag II

    Expression

    Mutation

    Promoter

    none

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAKTaq

    Plasmid

    #25712

    Purpose

    Bacterial expression of Taq polymerase

    Depositor

    David Engelke

    Article

    Engelke et al Anal Biochem. 1990 Dec . 191(2):396-400.

    Insert

    Taq polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pST39

    Plasmid

    #64009

    Purpose

    original polycistronic cloning vector for plasmid suite (generation I)

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJMP12042

    Plasmid

    #222354

    Purpose

    Tn7 integrative vector with PabstBR promoter expression cassette and aprR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

    Depositor

    Jason Peters

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFGL821

    Plasmid

    #58223

    Purpose

    For fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.

    Depositor

    Naweed Naqvi

    Article

    Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

    Insert

    hygromycin B phosphotransferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans trpC promoter

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVS1

    Plasmid

    #33143

    Depositor

    Michael Rosbash

    Article

    Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.

    Insert

    LacO (256 copies)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pHV739

    Plasmid

    #117477

    Purpose

    Expression of NMT2

    Depositor

    Richard Kahn

    Article

    Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

    Insert

    NMT2 (NMT2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMAK464

    Plasmid

    #140702

    Purpose

    NBαEGFP

    Depositor

    Takayuki Iwaki

    Article

    Iwaki et al Protein Expr Purif. 2020 Feb 13;170:1056

    Insert

    NBαEGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTP223

    Plasmid

    #13263

    Depositor

    Kenan Murphy

    Article

    Murphy et al BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.

    Insert

    Plac-gam-bet-exo
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. p15aC-GFP

    Plasmid

    #107862

    Purpose

    Control plasmid expressing GFP

    Depositor

    Sanford Simon

    Article

    Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

    Insert

    GFP
    

    Use

    Tags

    myc

    Expression

    Bacterial

    Mutation

    Promoter

    proD

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET3aTr

    Plasmid

    #64008

    Purpose

    Single donor or transfer plasmid for source of translation cassette for plasmid suite

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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