We narrowed to 3,906 results for: SPL;

1. GST-N1IC

   Plasmid

   #47612

   Purpose

   bacterial expression of GST-myc-Notch1 IC

   Depositor

   Urban Lendahl

   Article

   Beatus et al Development. 1999 Sep;126(17):3925-35.

   Insert

   Notch1 intracellular domain (Notch1 Mouse)
   

   Use

   Tags

   GST and myc

   Expression

   Bacterial

   Mutation

   contains amino acids 1753-2531

   Promoter

   tac

   Available since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

2. PEP113E

   Plasmid

   #97399

   Purpose

   binary construct of peroxisome-targeted mCherry with sfGFP11 tag for plant expression

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

   Insert

   mCherry-sfGFP11
   

   Use

   Tags

   Peroxisome target

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. SNV-gfp-IRES-MCS

   Plasmid

   #53122

   Purpose

   Retrovirus vector backbone that co-expresses GFP and a gene of interest, driven by the Spleen Necrosis Virus promoter; for use in chicken cells

   Depositor

   Jeanette Hyer

   Article

   Venters et al Dev Dyn. 2008 Nov;237(11):3260-9. doi: 1

   Type

   Empty backbone

   Use

   Retroviral; For expression in chicken cells

   Tags

   EGFP-IRES

   Expression

   Mutation

   Promoter

   SNV (spleen necrosis virus)

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEBB-II-I-GFP alpha isoform

   Plasmid

   #22146

   Depositor

   Ananda Roy

   Article

   Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

   Insert

   TFII-I (GTF2I Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

5. RFP-FKBP-U2AF35

   Plasmid

   #187969

   Purpose

   expresses U2AF35 with N-terminal tags RFP and FKBP

   Depositor

   Markus Wahl

   Article

   Vester et al Commun Biol. 2022 Jul 22;5(1):736. doi:

   Insert

   U2AF35 (U2af1 Mouse)
   

   Use

   Cmv promoter

   Tags

   mRFP-FKBP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. RFP-FKBP-PRPF38A

   Plasmid

   #187968

   Purpose

   expresses PRPF38A with N-terminal tags RFP and FKBP

   Depositor

   Markus Wahl

   Article

   Vester et al Commun Biol. 2022 Jul 22;5(1):736. doi:

   Insert

   Prp38A (PRPF38A Synthetic, Human)
   

   Use

   Cmv promoter

   Tags

   mRFP-FKBP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pB-bsd-Flag-Trl1

   Plasmid

   #126609

   Purpose

   Expresses N-terminal Flag-tagged yeast Trl1

   Depositor

   Xiaozhong Wang

   Article

   Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

   Insert

   tRNA ligase 1 (TRL1 Budding Yeast)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEBG-TFII-I gamma

   Plasmid

   #22156

   Depositor

   Ananda Roy

   Article

   Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

   Insert

   TFII-I (GTF2I Human)
   

   Use

   Tags

   GST and His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pSiMPlh_N + pSiMPlh_C

   Plasmid

   #134316

   Purpose

   Maintenance of 2 plasmids with a single antibiotic in E. coli

   Depositor

   Barbara Di Ventura

   Article

   Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

   Insert

   hygR_gp41-1 N-intein + hygR_gp41-1 C-intein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   P254Q in hygR_gp41-1 N-intein

   Promoter

   Available since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV/3' Box_(GLuc)_TOP2

    Plasmid

    #68435

    Purpose

    Transient expression of the "TOP2" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP2 construct
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEBB-II-I-GFP gamma isoform

    Plasmid

    #22148

    Depositor

    Ananda Roy

    Article

    Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pU6_(Gluc)_INT(GenPurpClon)

    Plasmid

    #68433

    Purpose

    General Purpose cloning vector for "INT"-like constructs under U6-promoter expression.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct
    

    Use

    CRISPR; Cloning vector for generating int-like co…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. cPE

    Plasmid

    #183549

    Purpose

    Expresses compact Prime Editor in mammalian cells

    Depositor

    Wen Xue

    Article

    Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

    Insert

    SpCas9 nickase (H840A), Reverse Transcriptase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-FRB-VMAc-CSarcin

    Plasmid

    #70223

    Purpose

    Expresses VMA intein with C-terminal portion of alpha-sarcin

    Depositor

    Perry Howard

    Article

    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

    Insert

    alpha Sarcin (C-terminal portion)
    

    Use

    Tags

    FKBP-rapamycin binding (FRB) domain of the mammal…

    Expression

    Mammalian

    Mutation

    Changed Asp 28 to Cys, deleted amino acids 1-27

    Promoter

    CMV

    Available since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pB-puro-V5-Trpt1

    Plasmid

    #126607

    Purpose

    Expresses N-terminal V5-tagged mouse Trpt1

    Depositor

    Xiaozhong Wang

    Article

    Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

    Insert

    tRNA 2' phosphotransferase 1 (Trpt1 Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEBB-II-I-GFP beta isoform

    Plasmid

    #22147

    Depositor

    Ananda Roy

    Article

    Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pU6_(GLuc)_INT[Spinach2]

    Plasmid

    #68430

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing the "Spinach2" aptamer
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFastBac-His-Flag-Timeless

    Plasmid

    #22892

    Depositor

    Aziz Sancar

    Article

    Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

    Insert

    Timeless (TIMELESS Human)
    

    Use

    Tags

    Flag and His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pM378

    Plasmid

    #173174

    Purpose

    Expresses C9-1 gRNA-12xMBS from hU6 promoter

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    C9-1 targeting gRNA-12xMBS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEBG-TFII-I alpha

    Plasmid

    #22154

    Depositor

    Ananda Roy

    Article

    Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Tags

    GST and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only