We narrowed to 5,570 results for: donor

1. pDNR-mCherry-T2A-3xPuroR-Frame 0

   Plasmid

   #206991

   Purpose

   Donor plasmid for Frame 0 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

   Depositor

   Alejandro Chavez

   Article

   Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   mCherry

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AICSDP-43: GJA1-mEGFP

   Plasmid

   #109120

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human GJA1

   Depositor

   Allen Institute for Cell Science

   Insert

   GJA1 Homology Arms with linker-mEGFP (GJA1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   May 4, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pAGD248

   Plasmid

   #219498

   Purpose

   Temperature sensitive replication (repA101ts) backbone encoding the retron recombineering operon (ncRNA, RT, CspRecT, mutLE32K)

   Depositor

   Seth Shipman

   Article

   Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

   Insert

   Eco1 ncRNA (rpoB donor), Eco1 RT, CspRecT, mutLE32K
   

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCVL SFFV d14GFP EF1s HA.NLS.Sce(opt)

   Plasmid

   #31476

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   SFFV d14 GFP donor EF1s I-SceI
   

   Use

   Lentiviral

   Tags

   HA and NLS

   Mutation

   GFP has 14 amino acids deleted from c terminus

   Available Since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEED-sfGFP

   Plasmid

   #219639

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   sfGFP SEED Cassette
   

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDNR-mCherry-T2A-3xPuroR-Frame 1

   Plasmid

   #206992

   Purpose

   Donor plasmid for Frame 1 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

   Depositor

   Alejandro Chavez

   Article

   Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   mCherry

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDNR-mCherry-T2A-3xPuroR-Frame 2

   Plasmid

   #206993

   Purpose

   Donor plasmid for Frame 2 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

   Depositor

   Alejandro Chavez

   Article

   Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   mCherry

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAVS1 LAMP1-mNeonGreen HRD

   Plasmid

   #207549

   Purpose

   Homologous recombination donor to integrate and expression cassette for LAMP1-mNEONGREEN in the AAVS1 locus of human cells

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   TET inducible LAMP1-mNeonGreen
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   Endogenous

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGFPmAID

   Plasmid

   #174213

   Purpose

   GFP::mAID donor backbone

   Depositor

   Masato Kanemaki

   Article

   Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-eGFP-pA

    Plasmid

    #107281

    Purpose

    donor vector backbone with constitutive eGFP

    Depositor

    Knut Woltjen

    Article

    Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

    Insert

    GFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. AICSDP-47: CTNNB1-mEGFP

    Plasmid

    #109119

    Purpose

    Homology arms and mEGFP-linker sequence for N-terminus tagging of human CTNNB1

    Depositor

    Allen Institute for Cell Science

    Insert

    CTNNB1 Homology Arms with mEGFP-linker (CTNNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    May 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. Halo-ATG16L1 HRD

    Plasmid

    #207537

    Purpose

    Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG16L1 locus in human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    HaloTag with internal PuroR cassette flanked by human ATG16L1 locus sequences
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMK382

    Plasmid

    #214373

    Purpose

    Tet-OsTIR1(F74G) (AAVS1 insertion donor)

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    Tet-OsTIR1(F74G)
    

    Expression

    Mammalian

    Promoter

    Tet ON 3G promoter

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTF-lacZ-rfp

    Plasmid

    #153037

    Purpose

    CRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    CRISPR array
    

    Expression

    Bacterial

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. AICSDP-29: LMNB1-mTagRFP-T

    Plasmid

    #114403

    Purpose

    Homology arms and mTagRFPT-linker sequence for N-terminus tagging of human LMNB1

    Depositor

    Allen Institute for Cell Science

    Insert

    LMNB1 Homology Arms with mTagRFPT-linker (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mTagRFPT-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Sept. 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pSEED-mScarlet3

    Plasmid

    #219640

    Purpose

    Permits GoldenGate assembly of donor plasmids to knock-in mScarlet3 (BsmBI) using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    mScarlet SEED Cassette
    

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Halo-ATG9A HRD

    Plasmid

    #203222

    Purpose

    Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG9A locus in human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    HaloTag flanked by human ATG9 locus sequences
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Bxb1-GA_CAG-mStayGold

    Plasmid

    #229802

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mStayGold
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSEED-ALFA:HA

    Plasmid

    #219646

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in ALFA and HA tags using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    ALFA:HA SEED Cassette
    

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSEED-sfGFP:ALFA

    Plasmid

    #219642

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP:ALFA using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    sfGFP:ALFA SEED Cassette
    

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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