We narrowed to 2,799 results for: fas

1. pJ414-PCY1:Y696G

   Plasmid

   #98156

   Purpose

   Expresses PCY1:R696G from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1:Y696G
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Changed tyrosine 696 to glycine

   Promoter

   T7

   Available Since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZA3

   Plasmid

   #158481

   Purpose

   Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 L17E with STOP codon

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   ZAR1 (ZAR1 Mustard Weed)
   

   Use

   Golden gate entry vector

   Mutation

   L17E, STOP codon

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZA2

   Plasmid

   #158480

   Purpose

   Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 D489V with STOP codon

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   ZAR1 (ZAR1 Mustard Weed)
   

   Use

   Golden gate entry vector

   Mutation

   D489V, STOP codon

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZA11

   Plasmid

   #158489

   Purpose

   Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 L17E without STOP codon

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   ZAR1 (ZAR1 Mustard Weed)
   

   Use

   Golden gate entry vector

   Mutation

   L17E no STOP codon

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZA9

   Plasmid

   #158487

   Purpose

   Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 without STOP codon

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   ZAR1 (ZAR1 Mustard Weed)
   

   Use

   Golden gate entry vector

   Mutation

   no STOP codon

   Available Since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZA4

   Plasmid

   #158482

   Purpose

   Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 L17E/D489V with STOP codon

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   ZAR1 (ZAR1 Mustard Weed)
   

   Use

   Golden gate entry vector

   Mutation

   L17E, D489V, STOP codon

   Available Since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZA10

   Plasmid

   #158488

   Purpose

   Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 D489V without STOP codon

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   ZAR1 (ZAR1 Mustard Weed)
   

   Use

   Golden gate entry vector

   Mutation

   D489V, no STOP codon

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZA12

   Plasmid

   #158490

   Purpose

   Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 D489V/L17E without STOP codon

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   ZAR1 (ZAR1 Mustard Weed)
   

   Use

   Golden gate entry vector

   Mutation

   L17E, D489V, no STOP codon

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNB682

   Plasmid

   #60143

   Purpose

   Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus-PEST
   

   Tags

   PEST degron

   Expression

   Yeast

   Mutation

   A4V

   Promoter

   MET17pr

   Available Since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXPR_051_dCas9-KRAB-HA

    Plasmid

    #228936

    Purpose

    All-in-one dCas9-KRAB-MeCP2 plasmid for cloning of custom sgRNA

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. IMPT-10314:GABBR2

    Plasmid

    #157851

    Purpose

    HA-3xFLAG-VFT-TMD-CC in pFastBac1 for Sf9 expression

    Depositor

    Raymond Stevens, Vadim Cherezov

    Article

    Shaye et al Nature. 2020 Jun 17. pii: 10.1038/s41586

    Insert

    GABBR2 (GABBR2 Human)
    

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC3.1_CMV_oROS-G_LF(C199S)

    Plasmid

    #216112

    Purpose

    Expresses the loss-of-function mutations C199S of the genetically encoded green fluorescent hydrogen peroxide sensor oROS-G in mamalian cells.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.01.31.578117

    Insert

    oROS-G_LF(C199S)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28b_H6-Smt3-AesC-GFP

    Plasmid

    #234486

    Purpose

    Superfolder GFP C-terminally fused to His-Smt3-tagged AesC split intein

    Depositor

    Henning Mootz

    Article

    Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:

    Insert

    C-terminal Fragment of the Aes123 PolB1 Split Intein
    

    Tags

    Hexahistidine Tag, Superfolder Green Fluorescent …

    Expression

    Bacterial

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP

    Plasmid

    #158962

    Purpose

    Cre-dependent Cas9-GFP expression

    Depositor

    Michael Brand

    Article

    Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

    Inserts

    hsp70l promoter
    floxed DsRed cassette
    Cas9-GFP
    

    Use

    CRISPR and Cre/Lox

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1/Puro-CAG-ASAP2s

    Plasmid

    #101274

    Purpose

    Genetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)

    Depositor

    Francois St-Pierre

    Article

    Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

    Insert

    ASAP2s
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. p(HA)HIF1alpha (P402A,P564A)

    Plasmid

    #52636

    Purpose

    mammalian expression of HA tagged HIF1alpha (full length) with P402A,P564A mutation

    Depositor

    Eric Huang

    Article

    Kageyama et al FASEB J. 2004 Jun;18(9):1028-30. Epub 20

    Insert

    HIF1alpha P402A, P564A (HIF1A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    P402A, P564A

    Promoter

    CMV

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-syn-dLight1.3b

    Plasmid

    #135762

    Purpose

    For expression of the genetically encoded dopamine sensor dLight1.3b

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Has Service

    AAV9

    Insert

    dLight1.3b
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-dLight1.2

    Plasmid

    #111068

    Purpose

    To generate Adeno-Associated viruses for expression of dLight1.2 under synapsin promoter

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Has Service

    AAV1 and AAV5

    Insert

    dLight1.2
    

    Use

    AAV

    Tags

    Flag tag

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRISPR-HOT-P2A-Clover-BlastR

    Plasmid

    #138568

    Purpose

    Universal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJ

    Depositor

    Hans Clevers

    Article

    Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

    Insert

    P2A-clover-BlastR
    

    Use

    CRISPR

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Tubulin-mGreenLantern

    Plasmid

    #164460

    Purpose

    Localization of mGreenLantern fluorescent protein to tubulin

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Tags

    Tubulin

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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