We narrowed to 55,910 results for: plasmid

1. pFREE_Amp

   Plasmid

   #92051

   Purpose

   Allows inducible expression of gRNAs and Cas9 for plasmid curing.

   Depositor

   Morten Norholm

   Article

   Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

   Inserts

   Cas9
   gRNA array
   

   Use

   CRISPR

   Promoter

   pRhamBAD and ptet

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFREE_Cm

   Plasmid

   #92052

   Purpose

   Allows inducible expression of gRNAs and Cas9 for plasmid curing.

   Depositor

   Morten Norholm

   Article

   Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

   Inserts

   Cas9
   gRNA array
   

   Use

   CRISPR

   Promoter

   pRhamBAD and ptet

   Available Since

   Aug. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pESC-TRP (V5 and HA)

   Plasmid

   #222488

   Purpose

   Co-expression of two genes in yeast with V5 and HA tags and TRP1 selection

   Depositor

   Tetsuo Matsuzaki, Kiyofumi Yamada

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Tags

   HA and V5

   Expression

   Yeast

   Available Since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pESC-LEU (V5 and HA)

   Plasmid

   #222486

   Purpose

   Co-expression of two genes in yeast with V5 and HA tags and LEU2 selection

   Depositor

   Tetsuo Matsuzaki, Kiyofumi Yamada

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Tags

   HA and V5

   Expression

   Yeast

   Available Since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFREE_Zeo

   Plasmid

   #92053

   Purpose

   Allows inducible expression of gRNAs and Cas9 for plasmid curing.

   Depositor

   Morten Norholm

   Article

   Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

   Inserts

   Cas9
   gRNA array
   

   Use

   CRISPR

   Promoter

   pRhamBAD and ptet

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pESC-URA (V5 and HA)

   Plasmid

   #222485

   Purpose

   Co-expression of two genes in yeast with V5 and HA tags and URA3 selection

   Depositor

   Tetsuo Matsuzaki, Kiyofumi Yamada

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Tags

   HA and V5

   Expression

   Yeast

   Available Since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-sCAG-eGFP-ACAP1

   Plasmid

   #203800

   Purpose

   AAV vector plasmid expressing human ACAP1 fused to eGFP under a short variant of the CAG (sCAG) promoter

   Depositor

   Bart Nieuwenhuis, Richard Eva

   Insert

   ACAP1 (ACAP1 Human)
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pESC-HIS (V5 and HA)

   Plasmid

   #222487

   Purpose

   Co-expression of two genes in yeast with V5 and HA tags and HIS3 selection

   Depositor

   Tetsuo Matsuzaki, Kiyofumi Yamada

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Tags

   HA and V5

   Expression

   Yeast

   Available Since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJB06

   Plasmid

   #190480

   Purpose

   Base Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis system

   Depositor

   Joseph Sorg

   Article

   Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

   Inserts

   Cas9
   XylR
   

   Use

   E. coli / b. subtilis - c. difficile shuttle vect…

   Promoter

   xylR-driven promoter

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJB07

    Plasmid

    #190481

    Purpose

    Targeting plasmid for 2-plasmid C. difficile mutagenesis system

    Depositor

    Joseph Sorg

    Article

    Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

    Inserts

    Upstream & Downstream pyrE deletion region
    gRNA
    

    Use

    E. coli / b. subtilis - c. difficile shuttle vect…

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pESC-ADE (V5 and HA)

    Plasmid

    #222489

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and ADE2 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Tags

    HA and V5

    Expression

    Yeast

    Available Since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAV-sCAG-eGFP-RAB11

    Plasmid

    #203799

    Purpose

    AAV vector plasmid expressing human RAB11 fused to eGFP under a short variant of the CAG (sCAG) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Insert

    RAB11 (RAB11A Human)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. SARS-CoV-2 Spike-d18

    Plasmid

    #164583

    Purpose

    A mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.

    Depositor

    Jason Sheltzer

    Insert

    SARS-CoV-2 Spike (S SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1256-1273

    Promoter

    EF1a

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Mouse 3' Hp1a AID GFP PuroR

    Plasmid

    #127897

    Purpose

    HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Mouse Hp1a Gene

    Depositor

    Mark Groudine

    Insert

    Hp1a (Cbx5 Mouse, Mustard Weed)
    

    Use

    Donor plasmid

    Tags

    GFP, AID, PuroR

    Mutation

    Inserting GFP AID 2A Puro into mouse 3' Hp1a

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFG036 + pFG051

    Plasmid

    #137997

    Purpose

    Transposon mutagenesis plasmid and the cI repressor expression plasmid

    Depositor

    Sébastien Rodrigue

    Article

    Neil et al Commun Biol. 2020 Sep 22;3(1):523. doi:

    Insert

    Tn5 tnp
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pESC-ARG (V5 and HA)

    Plasmid

    #222493

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and ARG1 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Tags

    HA and V5

    Expression

    Yeast

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pESC-MET (V5 and HA)

    Plasmid

    #222490

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and MET15 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Tags

    HA and V5

    Expression

    Yeast

    Available Since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pESC-LYS (V5 and HA)

    Plasmid

    #222491

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and LYS2 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Tags

    HA and V5

    Expression

    Yeast

    Available Since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pESC-TYR (V5 and HA)

    Plasmid

    #222492

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and TYR1 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Tags

    HA and V5

    Expression

    Yeast

    Available Since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pESC-THR (V5 and HA)

    Plasmid

    #222494

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and THR1 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Tags

    HA and V5

    Expression

    Yeast

    Available Since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge