We narrowed to 410 results for: px458

1. PX458-mcherry

   Plasmid

   #161974

   Purpose

   Expresses Cas9-p2a-mcherry (BbsI site silenced) and sgRNA

   Depositor

   Joanna Wysocka

   Article

   Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

   Insert

   Cas9-t2a-mcherry
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX458-AAVS1

   Plasmid

   #113194

   Purpose

   Encodes gRNA for human AAVS1

   Depositor

   Adam Karpf

   Insert

   AAVS1 (PPP1R12C, AAVS1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX458 V3

   Plasmid

   #226957

   Purpose

   CBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX458-HypaCas9

   Plasmid

   #190604

   Purpose

   PX458 (Plasmid #48138) with the N692A, M694A, Q695A, and H698A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Takahashi et al iScience. 2022 Nov 17;25(12):105619. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX458-DsRed2

   Plasmid

   #112219

   Purpose

   Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2

   Depositor

   Yinming Liang

   Article

   Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

   Insert

   DsRed2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   DsRed2 contains a silent nucleotide substitution …

   Promoter

   Available since

   July 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. PX458-sgEIF4EBP1

   Plasmid

   #128097

   Purpose

   CRISPR gRNA against human EIF4EBP1 (4EBP1) with Cas9 from S. pyogenes and 2A-EGFP

   Depositor

   Chi Van Dang

   Article

   Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

   Insert

   CRISPR sgRNA against human 4EBP1 (EIF4EBP1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX458-ECFP

   Plasmid

   #112220

   Purpose

   Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP

   Depositor

   Yinming Liang

   Article

   Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

   Insert

   ECFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX458-sgTSC2

   Plasmid

   #128098

   Purpose

   CRISPR gRNA against human TSC2 with Cas9 from S. pyogenes and 2A-EGFP

   Depositor

   Chi Van Dang

   Article

   Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

   Insert

   sgRNA against human TSC2 (TSC2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. px458 EQR

   Plasmid

   #101731

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. px458-CCND1

    Plasmid

    #172630

    Purpose

    Expresses a gRNA against CCND1 and Cas9 from S. pyogenes with 2A-EGFP

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    gRNA against CCND1 (CCND1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX458-sgRNA_Ago1_1

    Plasmid

    #73533

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago1

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX458-sgRNA_Ago1_2

    Plasmid

    #73534

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago1

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

13. pX458-sgRNA_Ago2_3

    Plasmid

    #73531

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago2

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX458-sgRNA_Ago2_4

    Plasmid

    #73532

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago2

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PX458-gR2A_ISO

    Plasmid

    #124808

    Purpose

    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a> plasmids to convert expression of rat IgG2a hybridomas to isotype of choice.

    Depositor

    Martijn Verdoes, Ferenc Scheeren

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    hSPCas9
    

    Use

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX458-sgRNA_Ago1_3

    Plasmid

    #73535

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago1

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

17. pX458-SLC35A1

    Plasmid

    #239952

    Purpose

    Encodes gRNA for human SLC35A1

    Depositor

    Jan Carette

    Article

    Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

    Insert

    SLC35A1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX458-MFSD6

    Plasmid

    #239951

    Purpose

    Encodes gRNA for human MFSD6

    Depositor

    Jan Carette

    Article

    Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

    Insert

    MFSD6
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX458-ITGB6

    Plasmid

    #235244

    Purpose

    Encodes gRNA for human ITGB6

    Depositor

    Jan Carette

    Article

    Qiao et al Nat Commun. 2024 Apr 24;15(1):3469. doi:

    Insert

    ITGB6 (ITGB6 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX458-MYADM

    Plasmid

    #235245

    Purpose

    Encodes gRNA for human MYADM

    Depositor

    Jan Carette

    Article

    Qiao et al Nat Commun. 2024 Apr 24;15(1):3469. doi:

    Insert

    MYADM (MYADM Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge