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Plasmid Kit#1000000189PurposeThe NT-CRISPR plasmid collection contains all plasmids to perform genome engineering in Vibrio natriegens using the NT-CRISPR method.DepositorAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only
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B. subtilis CRISPRi Essential Gene Knockdown Library
Plasmid Kit#1000000116PurposeB. subtilis CRISPRi Essential Gene Knockdown Library: Arrayed library that uses CRISPRi to sterically hinder transcription and therefore knock down expression of essential genes.DepositorAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
MultiGreen
Plasmid Kit#1000000250PurposeThe MultiGreen kit enables users to assemble GreenGate-compatible multigene plant transformation vectors.DepositorAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
The Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit
Plasmid Kit#1000000210PurposeToolkit contains Cas9-gRNA vectors, integration plasmids, and organellar localization markers as a set of plasmids for Cas9-mediated integration of tagged genes and markers into the yeast genome.DepositorAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Yamamoto Lab TALEN Accessory Pack
Plasmid Kit#1000000030PurposeContains modified pFUS array vectors and destination vectors that are designed for use with the Golden Gate TALEN and TAL Effector Kit.DepositorAvailable sinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
Endy/Shearwin pOSIP plasmid set
Plasmid Kit#1000000035Purpose11 Plasmids for use in clonetegration: method for decreasing time and effort needed for integration of DNA into prokaryotic chromosomes. Facilitates cloning, expression of difficult genetic elementsDepositorAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
S. cerevisiae Advanced Gateway Destination Vectors
Plasmid Kit#1000000011PurposeYeast (S. cerevisiae) Gateway Destination Vectors for tagging and gene expression. Has variety of promoters, origin of replications, auxotrophic markers, and N- or C-terminal fusion proteinsDepositorAvailable sinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
POMBOX MoClo YTK
Plasmid Kit#1000000251PurposeUse POMBOX for seamless and efficient assembly of single gene and multigene plasmids for genome integration in S. pombe.DepositorAvailable sinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMVP and pMAGIC Cloning Systems
Plasmid Kit#1000000155PurposepMVP and pMAGIC are cloning systems for adenoviral, lentiviral, expression, PiggyBac transposon, and Sleeping Beauty transposon vectors for transgene or RNAi delivery and dCas9-based engineering.DepositorAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
Golden GATEway Cloning Kit
Plasmid Kit#1000000054PurposeCombinatorial assembly method that combines Golden Gate (Type IIs endonucleases) cloning with Multisite Gateway (recombination) cloning to construct complex plasmids in an efficient and modular wayDepositorAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
FusX TALEN assembly system
Plasmid Kit#1000000063PurposeGolden Gate assembly of 15-16 RVD TALEN inserts in a single cloning step; designed to be used with high throughput roboticsDepositorAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
LIC TAL Effector Assembly Kit
Plasmid Kit#1000000023PurposeLIC (ligation-independent cloning) TALE repeat unit library of 2-mer fragments for assembly in an expression ready TALEN construct in 2 hierarchical assembly steps. Semiâautomated TALE generationDepositorAvailable sinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
EGFP/mCherry FLIM/FRET Kit
Plasmid Kit#1000000148DepositorAvailable sinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCri System
Plasmid Kit#1000000058PurposepCri System: plasmids for cytoplasmic and periplasmic/extracellular heterologous protein expression in Escherichia coli, Bacillus subtilis, and Pichia pastoris.DepositorAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
CRISPRainbow Kit for Multiplex Labeling
Plasmid Kit#1000000079PurposeCRISPR/Cas9 to tag up to 7 different genomic locations. Unique labeling system allows scientists to track DNA movement in live cells and study the structure of the genome in real time.DepositorAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
Wnt Open Source
Plasmid Kit#1000000022PurposeOpen Source Wnt: 95 plasmid library of all 19 Wnt clones. Collection contains full-length open reading frames (ORFs) for every human Wnt ligand.DepositorAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
Yeast Prototrophy Kit
Plasmid Kit#1000000085PurposePlasmids derived from the pHLUM mini-chromosome, designed to restore prototrophy (eg HIS3, LEU2, URA3, MET17 or LYS2 genes) in common yeast (S. cerevisiae) laboratory strains.DepositorAvailable sinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cancer Pathways ORFs Kit
Plasmid Kit#1000000072PurposeA set of vectors containing cDNAs for studying oncogenic signaling pathways. cDNAs are either wild-type, constitutively active mutants, or dominant negative mutants.DepositorAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
UbiGate E2 activity screen (set2)
Plasmid Kit#1000000145PurposeContains bacterial constructs for E3 autoubiquitination assay, including expression cassettes containing ubiquitin and the E1 UBA1 in combination with all Arabidopsis thaliana E2sDepositorAvailable sinceFeb. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
PUF Assembly Kit
Plasmid Kit#1000000051PurposeUsed to assemble custom-made RNA-binding proteins for recognition of 8-base long targets within RNA. The kit is based on the Pumilio and FBF (PUF) homology domain of the full-length human Pumilio 1.DepositorAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only