155 results

1. NT-CRISPR plasmid collection

   Plasmid Kit

   #1000000189

   Purpose

   The NT-CRISPR plasmid collection contains all plasmids to perform genome engineering in Vibrio natriegens using the NT-CRISPR method.

   Depositor

   Anke Becker

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

2. LIC TAL Effector Assembly Kit

   Plasmid Kit

   #1000000023

   Purpose

   LIC (ligation-independent cloning) TALE repeat unit library of 2-mer fragments for assembly in an expression ready TALEN construct in 2 hierarchical assembly steps. Semi‐automated TALE generation

   Depositor

   Veit Hornung

   Available since

   Jan. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

3. The Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit

   Plasmid Kit

   #1000000210

   Purpose

   Toolkit contains Cas9-gRNA vectors, integration plasmids, and organellar localization markers as a set of plasmids for Cas9-mediated integration of tagged genes and markers into the yeast genome.

   Depositor

   Vincent Martin

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

4. MoBiFC

   Plasmid Kit

   #1000000222

   Purpose

   The modular bimolecular fluorescence complementation (MoBiFC) toolkit allows for the analysis of protein-protein interactions in plants.

   Depositor

   Benjamin Field

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

5. UbiGate E2 activity screen (set2)

   Plasmid Kit

   #1000000145

   Purpose

   Contains bacterial constructs for E3 autoubiquitination assay, including expression cassettes containing ubiquitin and the E1 UBA1 in combination with all Arabidopsis thaliana E2s

   Depositor

   Marco Trujillo

   Available since

   Feb. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

6. UbiGate Components (set1)

   Plasmid Kit

   #1000000144

   Purpose

   Generate bacterial expression cassettes of up to six ORFs simultaneously; also contains ubiquitin, the E1 UBA1, an E2 collection, and a selection of E3s and their substrates for ubiquitination assays

   Depositor

   Marco Trujillo

   Available since

   Feb. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

7. CCSB-Broad Human Kinase ORF Collection

   Plasmid Kit

   #1000000014

   Purpose

   556 distinct human kinases and kinase-related protein ORFs in a pDONR-223 Entry Gateway vector. All clones are clonal isolates.

   Depositor

   David Root, William Hahn

   Available since

   July 16, 2011

   Availability

   Academic Institutions and Nonprofits only

8. POMBOX MoClo YTK

   Plasmid Kit

   #1000000251

   Purpose

   Use POMBOX for seamless and efficient assembly of single gene and multigene plasmids for genome integration in S. pombe.

   Depositor

   Tomáš Pluskal

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

9. MetClo DNA Assembly Kit

   Plasmid Kit

   #1000000153

   Purpose

   MetClo DNA Assembly Kit allows modular, hierarchical assembly of DNA fragments using IIS restriction enzyme. Includes 3 E.coli strains for BsaI, BpiI and LguI-based MetClo assembly.

   Depositor

   Christopher A. O'Callaghan

   Available since

   April 22, 2019

   Availability

   Academic Institutions and Nonprofits only

10. CRISPi Kit

    Plasmid Kit

    #1000000136

    Purpose

    CRISPi Kit contains elements for CRISPR/Cas9 mediated genome editing in yeast P. pastoris. Contains 7 different backbone (BB3) vectors with Cas9/ sgRNA expression cassettes with NTC, hphMX, or kanMX.

    Depositor

    Brigitte Gasser

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only