Skip to main content
Addgene
Showing: 1 - 20 of 21 results

  1. Barcoded self-reporting transposon calling card collection

    Plasmid Kit
    #1000000213
    Purpose
    'Calling Cards' technology using self-reporting transposons (SRTs) enables the identification of DNA-protein interactions through RNA sequencing.
    Depositor
    Rob Mitra
    Available since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only

  4. MetClo DNA Assembly Kit

    Plasmid Kit
    #1000000153
    Purpose
    MetClo DNA Assembly Kit allows modular, hierarchical assembly of DNA fragments using IIS restriction enzyme. Includes 3 E.coli strains for BsaI, BpiI and LguI-based MetClo assembly.
    Depositor
    Christopher A. O'Callaghan
    Available since
    April 22, 2019
    Availability
    Academic Institutions and Nonprofits only

  5. Alexandrov Cell-Free Protein Expression Kit (PEK)

    Plasmid Kit
    #1000000065
    Purpose
    Set of 89 ORFs cloned into the pCellFree vector to enable protein expression in any in vitro translation system; can perform statistically relevant benchmarking of cell-free expression systems
    Depositor
    Kirill Alexandrov
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. CCSB-Broad Human Kinase ORF Collection

    Plasmid Kit
    #1000000014
    Purpose
    556 distinct human kinases and kinase-related protein ORFs in a pDONR-223 Entry Gateway vector. All clones are clonal isolates.
    Depositor
    William Hahn, David Root
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  7. PRESTO-Tango Kit

    Plasmid Kit
    #1000000068
    Purpose
    Used to measure receptor activation for more than 300 G-protein coupled receptors (GPCRs) via a modified Tango beta-arrestin recruitment assay.
    Depositor
    Bryan Roth
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. B. subtilis CRISPRi Essential Gene Knockdown Library

    Plasmid Kit
    #1000000116
    Purpose
    B. subtilis CRISPRi Essential Gene Knockdown Library: Arrayed library that uses CRISPRi to sterically hinder transcription and therefore knock down expression of essential genes.
    Depositor
    Carol Gross
    Available since
    Sept. 1, 2017
    Availability
    Academic Institutions and Nonprofits only

  9. Zinc Finger Consortium Expression Vector Kit v1.0

    Plasmid Kit
    #1000000010
    Purpose
    Contains plasmids and strains for the bacterial two-hybrid (B2H) system used to screen zinc finger arrays for activity. Also called the Modular Assembly Accessory Reagents Kit.
    Depositor
    Keith Joung
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  10. Integrated Genomics Plasmids

    Plasmid Kit
    #1000000004
    Purpose
    Plasmids used in Integrated Genomics: A Discovery-Based Laboratory Course; please note that the C. elegans yeast two-hybrid cDNA library is unavailable
    Depositor
    Guy Caldwell
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  11. Sybody Generation Toolbox

    Plasmid Kit
    #1000000160
    Purpose
    The Seeger Lab protocol to select synthetic nanobodies – called sybodies – against (membrane) protein targets. This collection contains all plasmids necessary for the sybody generation.
    Depositor
    Markus Seeger
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only

  12. ONE-GO biosensors

    Plasmid Kit
    #1000000224
    Purpose
    This kit consists of ten ONE vector G protein Optical (ONE-GO) biosensor plasmids for the expression of BRET-based biosensors of G proteins activated by G-protein-coupled receptors (GPCRs).
    Depositor
    Mikel Garcia-Marcos
    Available since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only

  13. CRISPRecise Kit

    Plasmid Kit
    #1000000235
    Purpose
    CRISPRecise is a set of high-fidelity SpCas9 variants that can be used to efficiently edit any SpCas9 target with practically no detectable off-target effects.
    Depositor
    Ervin Welker
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only

  14. cpFRET

    Plasmid Kit
    #1000000021
    Purpose
    circular permutation-based fluorescence resonance energy transfer (cpFRET) biosensor toolkit: generate and optimize genetically encoded, unimolecular FRET sensors for ratiometric measurements
    Depositor
    Olivier Pertz
    Available since
    July 5, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. CRISPaint Gene Tagging Kit

    Plasmid Kit
    #1000000086
    Purpose
    Modular system for integrating large DNA fragments into defined genomic locations. Canonical NHEJ mechanism. For tagging of endogenous proteins with luciferase, fluorescent proteins, epitope tags
    Depositor
    Veit Hornung
    Available since
    Sept. 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  16. Multiplex Yeast Toolkit (MYT)

    Plasmid Kit
    #1000000229
    Purpose
    The Multiplex Yeast Toolkit (MYT) is a collection of 96 plasmids for genetically engineering the yeast, Saccharomyces cerevisiae.
    Depositor
    Tom Ellis
    Available since
    Nov. 9, 2023
    Availability
    Academic Institutions and Nonprofits only

  17. MoClo Yeast Toolkit

    Plasmid Kit
    #1000000061
    Purpose
    96 standardized parts used for bottom-up hierarchical assembly of single and multi-gene constructs for S. cerevisiae expression. Includes promoters, terminators, peptide tags, selection cassettes
    Depositor
    John Dueber
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. MoClo Plant Parts II and Infrastructure Kit

    Plasmid Kit
    #1000000135
    Purpose
    Compatible with the Modular Cloning (MoClo) system for hierarchical assembly of plant multigene constructs, with new phytobricks and vectors to connect Modular Cloning and Gateway Cloning platforms.
    Depositor
    Johannes Stuttmann
    Available since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  19. LIC TAL Effector Assembly Kit

    Plasmid Kit
    #1000000023
    Purpose
    LIC (ligation-independent cloning) TALE repeat unit library of 2-mer fragments for assembly in an expression ready TALEN construct in 2 hierarchical assembly steps. Semi‐automated TALE generation
    Depositor
    Veit Hornung
    Available since
    Jan. 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. MoChlo: Modular Cloning Chloroplast Toolbox

    Plasmid Kit
    #1000000156
    Purpose
    The MoChlo Toolbox consists of a library of standardized chloroplast-specific genetic modules for Golden-Gate cloning of synthetic operons into chloroplast transformation vectors.
    Depositor
    Scott Lenaghan
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 21 results