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Addgene
Showing: 21 - 35 of 35 results
  1. FX cloning Kit

    Plasmid Kit
    #1000000039
    Purpose
    Insert sequence of interest into a cloning vector and subclone into expression vectors, containing a variety of N- and C-terminal tags; for use in both prokaryotic and eukaryotic systems
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  2. Joung Lab REAL Assembly TALEN Kit

    Plasmid Kit
    #1000000017
    Purpose
    Used to assemble any combination of TALE repeats into arrays of any desired length using the REAL, REAL-Fast or FLASH assembly methods.
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  3. ScEnSor Kit

    Plasmid Kit
    #1000000215
    Purpose
    This three-step (Build - Transform - Assess) Saccharomyces cerevisiae kit is comprised of two modules (Engineering and Biosensor) that can be used independently or in combination.
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  4. FREQ-Seq Kit

    Plasmid Kit
    #1000000032
    Purpose
    For construction of barcoded, locus-specific libraries compatible with Illumina next generation sequencing. Allows for direct quantitative allele detection. 48 plasmid adaptors (barcoded M13F primer)
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  5. Start-Stop Assembly Toolkit

    Plasmid Kit
    #1000000152
    Purpose
    Start-Stop Assembly is a multi-part, modular DNA assembly method which is functionally scarless, suitable for combinatorial assembly, and ideal for metabolic engineering in diverse organisms.
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  6. GoldenPiCS Kit

    Plasmid Kit
    #1000000133
    Purpose
    GoldenPiCS: plasmid kit for engineering in P. pastoris (GoldenMOCS based). Overexpression of up to eight transcription units. 20 promoters, 10 terminators, 4 selection markers and 4 integration sites
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  7. MoClo Tool Kit

    Plasmid Kit
    #1000000044
    Purpose
    Eukaryotic multigene construct assembly in 3 steps using modular cloning (MoClo); Has 95 empty standardized genetic modules: promoters, 5' UTRs, signal peptides, coding sequences, and terminators
    Availability
    Academic Institutions and Nonprofits only
  8. MoClo Yeast Toolkit

    Plasmid Kit
    #1000000061
    Purpose
    96 standardized parts used for bottom-up hierarchical assembly of single and multi-gene constructs for S. cerevisiae expression. Includes promoters, terminators, peptide tags, selection cassettes
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  9. Fungal Toolkit for Modular Cloning (FTK)

    Plasmid Kit
    #1000000191
    Purpose
    Consists of 96 genetic parts as Modular Cloning-compatible entry vectors, including synthetic and native promoters, terminators, selection markers, and CRISPR components, for filamentous fungi.
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  10. CIDAR MoClo Parts Kit

    Plasmid Kit
    #1000000059
    Purpose
    CIDAR Modular Cloning (MoClo) kit uses modular DNA parts and enhanced MoClo protocols to enable rapid one-pot, multipart assembly, combinatorial design, and expression tuning in E. coli
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  11. EcoFlex MoClo Kit

    Plasmid Kit
    #1000000080
    Purpose
    Golden Gate kit for E. coli synthetic biology; consists of a modular library that uses MoClo assembly standard for a variety of uses, e.g. recombinant protein purification, cell-free circuit design
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  12. UbiGate Components (set1)

    Plasmid Kit
    #1000000144
    Purpose
    Generate bacterial expression cassettes of up to six ORFs simultaneously; also contains ubiquitin, the E1 UBA1, an E2 collection, and a selection of E3s and their substrates for ubiquitination assays
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  13. Diatom uLoop Assembly Kit

    Plasmid Kit
    #1000000238
    Purpose
    The collection contains a selection of diatom promoters and terminators from the organism Phaeodactylum tricornutum on vectors compatible with the uLoop cloning system.
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  14. Ekker Lab TALEN kit

    Plasmid Kit
    #1000000038
    Purpose
    Contains all 256 possible combinations of pFUS_B4 clones for Golden Gate assembly of 15-RVD TALENs for high-throughput mutagenesis
    Depositor
    Availability
    Academic Institutions and Nonprofits only
  15. RAS Pathway 2.0 Clone Collection

    Plasmid Kit
    #1000000070
    Purpose
    Collection contains 180 genes in the “RAS pathway version 2.0”, a crowdsourced pathway map of the RAS signaling pathway; For use with Gateway cloning or FNLCR Combinatorial Cloning Platform
    Depositor
    Availability
    Academic Institutions and Nonprofits only
Showing: 21 - 35 of 35 results