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ALIX(P801G)-Strep

Plasmid

#180024

Purpose

Codon-optimized bacterial expression plasmid. Expresses ALIX(1-868, P801G)-Strep. P801G mutation facilitates expression in E. coli.

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

ALIX(1-868, P801G)-Strep (PDCD6IP Human)

Tags

Strep tag (WSHPQFEK)

Expression

Bacterial

Mutation

P801G

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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MERS-coV-NSP3-c001

Plasmid

#173085

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

rep (orf1ab Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (MERS-CoV))

Tags

mCerulean

Expression

Bacterial

Mutation

D1109-D1275, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCS-PluxIlux

Plasmid

#47641

Purpose

wt luxI promoter in pCS26

Depositor

Cynthia Collins

Article

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

PluxI

Use

Synthetic Biology

Expression

Bacterial

Promoter

PluxI

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only
pCoofy16

Plasmid

#43988

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His10-NusA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-XIAP1-351

Plasmid

#8342

Depositor

Jon Ashwell

Article

Yang et al Science 2000 May 5;288(5467):874-7.

Insert

XIAP1-351 (XIAP Human)

Tags

GST

Expression

Bacterial

Mutation

deleted amino acids 352-496

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pBV58

Plasmid

#204603

Purpose

ERG11-3X Minidegron-5X FLAG-HIS3MX6

Depositor

Scott Moye-Rowley

Insert

Candida glabrata ERG11 (ERG11 )

Tags

auxin-inducible degron

Expression

Bacterial

Available Since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph

Plasmid

#218366

Purpose

Empty backbone for Tn7-site insertion

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMApra-Ptol

Plasmid

#218359

Purpose

Empty vector with XylS-Pm expression system

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph-Ptol

Plasmid

#218369

Purpose

Empty backbone for Tn7-site insertion with XylS-Pm expression system

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available Since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

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pTriExEKAR2G1

Plasmid

#40174

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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