30,602 results

1. pSum-LacZ-gfp

   Plasmid

   #109285

   Purpose

   Expresses gfp in mycobacteria

   Depositor

   Nicolai van Oers

   Article

   Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GFP modified for expression in prokaryotes; inclu…

   Promoter

   LacZalpha

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET His6 ProteinG TEV LIC cloning vector (1P)

   Plasmid

   #29660

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis, Protein G, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDule-Tet3.0

   Plasmid

   #174080

   Purpose

   Tetrazine 3.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

   Depositor

   Ryan Mehl

   Article

   Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-

   Insert

   Tet3.0 tRNA synthetase and tRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   L270G, Y271L, N311G, C313A

   Promoter

   Available Since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX4T1-human beta-catenin full length

   Plasmid

   #132609

   Purpose

   expresses human beta-catenin full length in E. coli

   Depositor

   Wenqing Xu

   Article

   Xing et al Structure. 2008 Mar;16(3):478-87. doi: 1

   Insert

   beta-catenin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-MCN-10His-StayGold

   Plasmid

   #211360

   Purpose

   Bacterial expression of the bright and photostable StayGold fluorescent protein. Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

   Depositor

   Mohan Balasubramanian

   Article

   Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

   Insert

   StayGold
   

   Use

   Tags

   10xHis-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-TS2126 RnlA-Strep

   Plasmid

   #159350

   Purpose

   Express TS2126 RNA ligase with His6 tag and Strep tag at N and C terminal, respectively

   Depositor

   Takashi Ito

   Article

   Hisano et al BMC Biol. 2021 Oct 14;19(1):225. doi: 10

   Insert

   TS2126 RNA ligase
   

   Use

   Tags

   His6 and Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACYCT2

   Plasmid

   #45799

   Depositor

   Luc Ponchon

   Article

   Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMOD3-proD/Lux-nat

   Plasmid

   #120324

   Purpose

   Bacterial luminescence

   Depositor

   James Kirby

   Article

   Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

   Insert

   Lux operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   proD

   Available Since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. PET-B-Sniper SpCas9-NLS-6xHis

   Plasmid

   #207377

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-Sniper SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F539S, M763I, K890N, amino acids 1005-1013 replac…

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAB1

    Plasmid

    #62547

    Purpose

    broad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.

    Depositor

    Michael Franklin

    Article

    Walters MC et al Antimicrob Agents Chemother. 2003 Jan;47

    Insert

    eGFP
    

    Use

    Broad host range plasmid

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTrc

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. PTP1B human

    Plasmid

    #102719

    Purpose

    human PTP1B catalytic domain (residues 1-301) in RP1B vector

    Depositor

    Wolfgang Peti

    Article

    Choy et al Mol Cell. 2017 Feb 16;65(4):644-658.e5.

    Insert

    Protein Tyrosine Phosphatase 1B (PTP1B) (PTPN1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-16b-10His-TEV-MCES_VACCA_D1_D12

    Plasmid

    #203671

    Purpose

    The D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavage

    Depositor

    Stephen Cusack

    Article

    Kyrieleis et al Structure. 2014 Mar 4;22(3):452-65. doi:

    Insert

    MCES_VACCA_D1_D12
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJ4M_hnRNPA2_FL_WT

    Plasmid

    #139109

    Purpose

    Express FL hnRNPA2 with a C-terminal MBP tag

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRSET FLIPPi-200u

    Plasmid

    #13555

    Depositor

    Wolf Frommer

    Article

    Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

    Insert

    FLIPPi G162A
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    G162A in binding protein

    Promoter

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. CAPN1

    Plasmid

    #25607

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CAPN1 (CAPN1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids 29-360

    Promoter

    Available Since

    Oct. 25, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pK27Sumo_His-SUMO-nsp12 (SARS-CoV-2)

    Plasmid

    #169188

    Purpose

    To express His-SUMO-nsp12 (SARS-CoV-2) in E. coli

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    14His-SUMO-nsp12 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    14His-SUMO (Ulp1-cleavable)

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTOX12

    Plasmid

    #127457

    Purpose

    Encodes Tse2 toxin. Gent-R

    Depositor

    Matthew Waldor

    Article

    Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

    Insert

    tse2 toxin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pRha

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUCSh5vio-2300

    Plasmid

    #170987

    Purpose

    Encodes sgRNA target 2300 and Gentamicin resistance - violacein pathway transposon.

    Depositor

    Christopher Reisch

    Article

    Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

    Insert

    Sh-CAST sgRNA 2300 violacein
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    placI

    Available Since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSELIS-RamR

    Plasmid

    #202631

    Purpose

    Enables dual growth and fluorescence based enrichment of RamR biosensors

    Depositor

    Andrew Ellington

    Article

    d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

    Insert

    Sh ble
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lambda promoter

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSET-RCaMP1h

    Plasmid

    #42874

    Purpose

    a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby

    Depositor

    Loren Looger

    Article

    Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33

    Insert

    Red Fluorescent Calcium binding Protein
    

    Use

    Tags

    EK (Enterokinase) Recognition Site, His Tag (6x),…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge