31,793 results
-
Plasmid#162666PurposepET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker
TagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable SinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET StrepII TEV LIC cloning vector (1R)
Plasmid#29664DepositorTypeEmpty backboneTagsStrep II and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDiGc
Plasmid#59322PurposeInducible expression of dsRed and constitutive expression of GFP in bacteriaDepositorInsertsEGFP
dsRed
ExpressionBacterialPromoterPbad and rpsMAvailable SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDx_mScarlet3-SYFP2
Plasmid#189764PurposeDual expression vector for bacteria and mammalian cells producing mScarlet3-SYFP2 fusion protein for FRET analysisDepositorInsertmScarlet3-SYFP2
Tags6xHisExpressionBacterial and MammalianPromoterCMV & RhaAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPET-PKR/PPase
Plasmid#42934DepositorAvailable SinceApril 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
iMbody_pSANG10-3F
Plasmid#184496PurposeExpresses iMbody-6xHis-3xFLAG in BL21(DE3) bacteriaDepositorInsertiMbody
Tags3xFLAG, 6xHis, and pelB leaderExpressionBacterialAvailable SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM5404
Plasmid#132660PurposeRSF1010-based broad host-range plasmid with additional RK2 bom siteDepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-1-LARP7
Plasmid#113545PurposeProduces an N-terminal GST-tagged full length LARP7 protein.DepositorAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHnCBS1D
Plasmid#52065Purposeexpresses nine genes from the H. neapolitanus carboxysome operon and CSOS1D; IPTG-inducibleDepositorInsertCbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B, CSOS1D
ExpressionBacterialAvailable SinceApril 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDL52 (clone76)
Plasmid#188579PurposeHigh-yield production of coenzyme F420 in E. coliDepositorInsertsribA
ribD
yigB
fbiC
cofC
cofD
cofI
cofE
ExpressionBacterialMutationCodon optimization, synthetic ribosome binding si…PromoterT7 variantsAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRE140
Plasmid#118492Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertTagBFP2
ExpressionBacterialAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PFO
Plasmid#92109PurposeRecombinant expression in E.ColiDepositorInsertPFO
Tags6xHisExpressionBacterialMutationDelta27aaAvailable SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNP1950
Plasmid#222729PurposeJ23119-BsaI_bRNA Golden Gate Landing Pad-HDV; T7-IS621 RecombinaseDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PCMT1_PCMT
Plasmid#109932PurposeProtein expression and purification of PCMT1_PCMTDepositorInsertPCMT1_PCMT
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSET-4xTR-TUBE
Plasmid#110312PurposeE. coli expression and purification of 4xTR-TUBEDepositorInsert4xTR-TUBE (UBQLN1 Human)
TagsN-terminal His6-T7 tagExpressionBacterialMutationAll Arg residues in the UBA domain are mutated to…PromoterT7Available SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
Skp
Plasmid#213139PurposeExpresses chaperone protein SKPDepositorAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1955
Plasmid#84830PurposeMinimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic intronsDepositorInsertC. elegans codon optimized tdTomato
ExpressionBacterial and WormPromoterPeft-3Available SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD-iLACCO1
Plasmid#218817PurposeBacterial expression of genetically encoded green fluorescent L-lactate biosensor with mild affinityDepositorInsertiLACCO1
ExpressionBacterialAvailable SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-iLACCO1.1
Plasmid#218818PurposeBacterial expression of genetically encoded green fluorescent L-lactate biosensor with low affinityDepositorInsertiLACCO1.1
ExpressionBacterialAvailable SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-iLACCO1.2
Plasmid#218819PurposeBacterial expression of genetically encoded green fluorescent L-lactate biosensor with high affinityDepositorInsertiLACCO1.2
ExpressionBacterialAvailable SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only