30,602 results

1. pLG033 (ietAS)

   Plasmid

   #157911

   Purpose

   Expresses the ietAS defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   ietAS (ATPase + serine protease)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pS24·Pm-GFP

   Plasmid

   #122594

   Purpose

   Expresses GFP under the regulatory control of Pm. Expression needs additional xyls gene

   Depositor

   Pablo Ivan Nikel

   Article

   Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

   Insert

   Pm promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pm

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD-KIRIN1-GR

   Plasmid

   #107417

   Purpose

   Bacterial Expression plasmid for potassium ion raitometric indicator KIRIN1-GR

   Depositor

   Robert Campbell

   Article

   Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

   Insert

   KIRIN1-GR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. L4440_BioBrick-hif-1-sgRNA-A

   Plasmid

   #177784

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   hif-1 (hif-1 Nematode)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmRFP_LAP2beta_IRES_puro2b

   Plasmid

   #21047

   Depositor

   Daniel Gerlich

   Article

   Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

   Insert

   LAP2_beta (TMPO Rat)
   

   Use

   Tags

   mRFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   July 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pProEx RalA(G23C) CL

   Plasmid

   #224263

   Purpose

   Protein purification of RalA (G23C)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   RalA (RALA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G23C

   Promoter

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETduet+sfGFP-TetR-p300HAT

   Plasmid

   #157795

   Purpose

   Expression of sfGFP-TetR-p300HAT fusion protein

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   sfGFP-TetR-p300 (EP300 Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. M57 pPET28a-TEV-full-length human beta catenin

   Plasmid

   #17198

   Depositor

   Randall Moon

   Insert

   beta catenin (CTNNB1 Human)
   

   Use

   Tags

   HIS

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. T4 Lysozyme WT

   Plasmid

   #18110

   Depositor

   Brian Matthews

   Insert

   T4 Lysozyme (e Bacteriophage T4)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 26, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ubiquitin (1-76; T66V, L67N)

    Plasmid

    #110755

    Purpose

    Bacterial expression of human Ubiquitin (1-76; T66V, L67N)

    Depositor

    David Komander

    Article

    Gladkova et al EMBO J. 2017 Dec 15;36(24):3555-3572. do

    Insert

    UBB (UBB Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T66V, L67N

    Promoter

    T7

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-3c-rhVTN-NC

    Plasmid

    #30226

    Depositor

    James Thomson

    Article

    Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

    Insert

    rhVitronectin with N and C terminus cleavages (VTN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deleted amino acids: 399-478, 20-62

    Promoter

    Available Since

    Sept. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSG15-pBAD-tetO-deGFP

    Plasmid

    #102450

    Purpose

    Expresses deGFP fluorescent protein in E. coli. The expression is controlled by a pBAD-tetO combinatorial promoter.

    Depositor

    Richard Murray

    Article

    Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

    Insert

    deGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD-tetO

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pnCS_SEPT7_SEPT9_i1-TEV-Strep

    Plasmid

    #174500

    Purpose

    bacterial co-expression of human SEPT7 and of human SEPT9_i1

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    

    Use

    Tags

    TEV-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. His-FGE

    Plasmid

    #75147

    Purpose

    Expression vector for His(6) tagged Formylglycine Generating Enzyme (FGE) in bacterial host.

    Depositor

    Richard Fishel

    Article

    Liu et al Sci Rep. 2015 Nov 19;5:16883. doi: 10.10

    Insert

    FGE
    

    Use

    Tags

    Histidine tag and Thrombin

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    T7

    Available Since

    July 8, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pDuEx2-NIR-GECO1

    Plasmid

    #113680

    Purpose

    Bacterial and mammalian expression plasmid for near-infrared fluorescent Ca2+ indicator NIR-GECO1

    Depositor

    Robert Campbell

    Article

    Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

    Insert

    NIR-GECO1
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS6

    Plasmid

    #55752

    Purpose

    Produces T7 RNA Polymerase.

    Depositor

    Matthew Bennett

    Article

    Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

    Insert

    T7 RNA Polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOPINK-OTUD2 (full-length, aa 1-348)

    Plasmid

    #61409

    Purpose

    Expresses human OTUD2 (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD2 (YOD1 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSFDuet-1_VNp-His6

    Plasmid

    #182386

    Purpose

    Bacterial expression of Vesicle Nucleating peptide

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-His
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Human PNP his-tagged

    Plasmid

    #64076

    Purpose

    Expression of human Purine Nucleoside Phosphorylase

    Depositor

    Vern Schramm

    Article

    Lewandowicz et al J Biol Chem. 2003 Aug 22;278(34):31465-8

    Insert

    Purine Nucleoside Phosphorylase (PNP Human)
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pTriEx-mCherry-Zdk1

    Plasmid

    #81057

    Purpose

    mammalian expression of Zdk1

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk1
    

    Use

    Tags

    6x His tag, FLAG, and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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