33,441 results
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Plasmid#173442PurposepRS316-mNeonGreen-CBP-2XFLAG-HYGB, contains C. neoformans optimized mNeonGreen and HYG marker for use as PCR templateDepositorInsertmNeonGreen-CBP-2XFLAG-HYGB
TagsCBP-2xFLAGExpressionBacterial and YeastAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHN1002
Plasmid#178831PurposeBacterial expression for human Saposin ADepositorInsertartificial gene encoding human Saposin A (AR Human)
TagsHis8, 3CExpressionBacterialMutationCodon-usage was optimized for bacterial expressio…PromoterT7Available sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_IGF2BP2a-Halo
Plasmid#175309PurposeProduction of HaloTag fusion proteinDepositorPublicationAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB219
Plasmid#164125Purposep15A backbone with PhlF binding sitesDepositorInsertsfGFP
ExpressionBacterialPromoterJ23105Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPSU30
Plasmid#173830PurposeExpresses 30 kD Penn State ladder proteinDepositorInsertHSTPABPACS100BCBP
ExpressionBacterialPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPSU10
Plasmid#173827PurposeExpresses 10 kD Penn State ladder proteinDepositorInsertSTRHSTPAB
ExpressionBacterialPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPSU60
Plasmid#173833PurposeExpresses 60 kD Penn State ladder proteinDepositorInsertHSTPABPACRCC1
ExpressionBacterialPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GST-His6-1_HBD
Plasmid#171869PurposeExpresses a specific DNA-RNA hybrid sensor protein with the HBD domain of RNase H1 in bacterial cellsDepositorInsertHybrid binding domain of Rnase H1
TagsGST- and His10-tagExpressionBacterialMutationWTAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-SP44-mScarlet-I
Plasmid#163756PurposeExpresses Streptomyces codon-optimised mScarlet-IDepositorInsertmScarlet-I
UseSynthetic BiologyTagsFlag and FlAsH tagsExpressionBacterialPromoterSP44Available sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAT203
Plasmid#134880PurposeBacterial expressionDepositorInsertmRFP1
UseSynthetic BiologyExpressionBacterialAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-AviTag-DogCatcher-MBP
Plasmid#171928PurposeExpresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.DepositorInsertAviTag-DogCatcher-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationDogCatcher added to N-terminus of MBP via a GSGES…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTetR-LasR-pLuxR-GFP
Plasmid#171159PurposeSensing Device with Reporter proteinDepositorInsertslasR
gfp
UseSynthetic BiologyExpressionBacterialPromoterpLux and pTetAvailable sinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-26b-Nb127D01-ALFA-His
Plasmid#171568PurposeBacterial expression of ALFA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-ALFA-His)DepositorPublicationInsertNb127D01-ALFA-His (CXCR2 Human)
TagsALFA-tag/His-tag and PelB signal peptideExpressionBacterialAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
FHL-cpmTq2-Calcium-lifetime-sensor
Plasmid#129628PurposeGenetically encoded calcium sensor (GECI) that reports with lifetime and intensity changesDepositorInsertFHL-cpmTq2-Calcium-lifetime-sensor
Tags6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …ExpressionBacterial and MammalianPromoterCMV,&RhamnoseAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHEA
Plasmid#168297PurposeBacterial surface display of polypeptides (nanobodies) in E. coliDepositorInsertEnterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
Tags6xHis, E-tag, and PelB signal peptideExpressionBacterialPromoterLacI-PlacAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
His-TEV-PRC1
Plasmid#167539PurposeFor expression of His6-TEV-PRC1 in bacteria.DepositorAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-LahT150
Plasmid#166785PurposeCleavage of double glycine motif in peptides to remove the leader sequenceDepositorInsertLahT150
TagsHis6ExpressionBacterialPromoterT7Available sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBAD-eLACCO1.1
Plasmid#167944PurposeFluorescent biosensor for extracellular L-lactateDepositorInserteLACCO1.1
ExpressionBacterialPromoteraraBADAvailable sinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPEPZ-sgRNAclone
Plasmid#141090PurposeThis vector is designed for efficient cloning of sgRNAs by Golden Gate assembly. The sgRNA insertion leads to replacement of mCherry, resulting in loss of red color of E. coli colony.DepositorInsertmCherry
UseCRISPRExpressionBacterialPromoterp3Available sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pExp-DsbC
Plasmid#112564PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbC (leaderless thiol:disulfide interchange protein DsbC from E. coli) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneTags8xHis-DsbC and Strep tagExpressionBacterialAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only