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Plasmid#75288PurposeExpresses proteins in the cytoplasm as fusions to a high-binding mutant (A313V) maltose-binding proteinDepositorTypeEmpty backboneUseTagsmaltose-binding proteinExpressionBacterialMutationPromotertacAvailable sinceJuly 19, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits
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V72 ELP
Plasmid#68938PurposeElastin-like Polypeptide (VPGxG) with 72 repeats containing Valine guest residueDepositorInsertV72 ELP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
human p53-(355-393)
Plasmid#24870DepositorInsertp53 (TP53 Human)
UseTagsGSTExpressionBacterialMutationContains aa 355-393.PromoterAvailable sinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pQE80L-IL2 C125S
Plasmid#104348PurposeExpresses human IL-2 (C125S)DepositorInsertHuman IL-2 (C125S) (IL2 Human)
UseTagsMRGSHHHHHGS-tagExpressionBacterialMutationC125S mutation (TGT -> TCC)PromoterT5Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEVOL_PylRS(WT)-tRNA
Plasmid#223512PurposePylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codonDepositorInsertsUseTags6xHisExpressionBacterialMutationPromoteraraBAD, rrnB, and glnS and proKAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
Fusion of Dengue2 NS2B-NS3
Plasmid#204800PurposeBacterial expression of a fusion of Dengue2 NS2B-NS3 proteinsDepositorInsertDengue2 NS2B-NS3 fusion
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJUMP18-Histag_C
Plasmid#127035PurposeBasic Part C- C-terminus; polyhistidine tagDepositorInsertPart His6_C
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SARA-no stop
Plasmid#67347PurposehSara without stop codon in Gateway Entry vectorDepositorInsertSARA (SAR1B Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-RhoA-wt_mScarlet-i_SGFP2
Plasmid#85071PurposeGreen/red FRET biosensor to monitor RhoA nucleotide loading state and activityDepositorInsertRhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
UseTags6xHis and mScarlet-i and SGFP2ExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
AARS_C1_pNIC-Bio3
Plasmid#153046PurposeExpression construct for producing Avi tagged Alanyl-tRNA synthetaseDepositorInsertAARS (AARS Human)
UseTagsAvi and His6-TEVExpressionBacterialMutationPromoterT7Available sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET3a-Ub-K63R
Plasmid#18898DepositorInsertUbiquitin-K63R (UBC Human)
UseTagsExpressionBacterialMutationLysine 63 is replaced with a Argnine residuePromoterAvailable sinceSept. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET21b-P35
Plasmid#11808DepositorInsertP35
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pK27Sumo_His-SUMO-nsp7 (SARS-CoV-2)
Plasmid#169186PurposeTo express His-SUMO-nsp7 (SARS-CoV-2) in E. coliDepositorInsert14His-SUMO-nsp7 (ORF1ab SARS-CoV-2)
UseTags14His-SUMO (Ulp1-cleavable)ExpressionBacterialMutationCodon optimised for E. coliPromoterT5Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p-1-BCCP-PupE
Plasmid#113403PurposeExpresses BCCP-PupE in bacteriaDepositorInsertBCCP-PupE
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Ec.coaD (pESC106)
Plasmid#50388PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.DepositorInsertPPAT
UseTags6xHis-tagExpressionBacterialMutationPromoterT7Available sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLG002 (BREX type I)
Plasmid#157880PurposeExpresses the BREX type I defense systemDepositorInsertBREX type I
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLC-284
Plasmid#124294PurposeA low copy plasmid constitutively expressing vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.DepositorInsertvsfGFP-9
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBPHLWL-LexA
Plasmid#26258DepositorInsertLexA
UseDrosophila transgenesisTagsExpressionBacterial and InsectMutationPromoterAvailable sinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1_4xUb
Plasmid#199779PurposeUsed for the expression of 4X human ubiquitin in tandem (SMC interal no. 538)DepositorInsert4X Ubiquitin in Tandem
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
fSAP24-GST
Plasmid#11202DepositorInsertSAP24 (THOC7 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2006AvailabilityAcademic Institutions and Nonprofits only