32,401 results
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Plasmid#58311PurposeShuttle vector: contains constitutive mRFP1, compatible as reporter for E. coli/B. subtilis. See pSEVA3b61 for more info.DepositorInsertmRFP1
UseSynthetic BiologyExpressionBacterialAvailable SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
His-MBP_G4G6 in pRSF
Plasmid#188454PurposeExpress in E. coli human gelsolin segments 4 through 6 with N-terminal 6xHis and MBP tags.DepositorAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET_sfCherry(1-10)_32aalinker_sfCherry(11)
Plasmid#83030PurposeBacterial expression vector for split sfCherry screeningDepositorInsertsfCherry(1-10)_32aalinker_sfCherry(11)
ExpressionBacterialPromoterT7 promoterAvailable SinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT85_P438-mEOS3.2_GentaR
Plasmid#173894PurposeTransposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the P438 promoterDepositorInsertmEos3.2
ExpressionBacterialPromoterP438Available SinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE80L SpyTag-ELP-SpyTag DK
Plasmid#112635PurposeSpyTag linked by an elastin-like polypeptide to non-reactive SpyTag DKDepositorInsertSpyTag-ELP-SpyTag DK
TagsHis6ExpressionBacterialMutationContains central cysteine. Reactive aspartic acid…PromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXT130_TwinStrep-SUMO-ShTnsC
Plasmid#135526PurposeProtein expression construct for ShTnsCDepositorInsertShTnsC
TagsTwinStrep-SUMOExpressionBacterialAvailable SinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHen2 Avi-tag-linker-p3
Plasmid#119819PurposeFor cloning and subsequent expression of peptide fragments fused with Avi-tag (for biotinylation) and with pIII protein of M13K07 bacteriophageDepositorInsertlinker with Avi-tag and p3
TagsAvi-tagExpressionBacterialAvailable SinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJB158
Plasmid#248751PurposePopuA+20xTetO biosensor cicuit in pNBU2 integrative Bacteroides plasmidDepositorInsertPBfP1E6-RBS1PLP-mScarletI3-rrnBT2-20xTetO-T7Te-rrnBT1-PopuA-TetR-rrnBT2-pTetR56-RBS1PLP-mGL-rrnBT2
ExpressionBacterialAvailable SinceJan. 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
pEM351
Plasmid#248763Purpose80xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate AssemblyDepositorInsertT7Te-rrnBT1-80xTetO
ExpressionBacterialAvailable SinceJan. 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
pEM414
Plasmid#248766Purpose20xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate AssemblyDepositorInsertT7Te-rrnBT1-20xTetO
ExpressionBacterialAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
EV-A71 isolate 4643-TW98 2A precursor protease with C110A and four aa at N-term to create a VP1-2A junction in active site
Plasmid#228633PurposeBacterial expression of the A71 2A protease with ITTL at the N-terminal and a C110A mutation used for crystallization in P 6 1 (PDB 9FGO)DepositorInsertEnterovirus Coxsackievirus A71 2A protease
TagsHis-SUMOExpressionBacterialMutationC110AAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
PPE18-ALFA
Plasmid#231247PurposeExpresses a C-terminally tagged PPE18 (ALFA tag)DepositorInsertPPE18-ALFA (PPE18 Mycobacterium tuberculosis)
TagsALFA TagExpressionBacterialPromoterhsp60Available SinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-mEGFP(A206K)-TwStrep
Plasmid#208622Purposefor E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) cloneDepositorInsertmEGFP(A206K)
Tags6xHis and Twin StrepExpressionBacterialMutation(compared with pEGFP-C1, protein level) A206KPromoterT7Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0919:Dendra
Plasmid#174936PurposeC-term fluorescent fusionDepositorInsertMSMEG_0919
UseIntegrating mycobacterial expression vector attl5…TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialPromoterpmycTetOAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_5482:Dendra
Plasmid#167437PurposeC-term fluorescent fusionDepositorInsertMSMEG_5482
UseIntegrating mycobacterial expression vector attl5…TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialPromoterpmycTetOAvailable SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIVEX2.3 TEM-7
Plasmid#16562DepositorAvailable SinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-GPD1
Plasmid#83911Purposestable overexpressionDepositorInsertglycerol-3-phosphate dehydrogenase 1 (GPD1 Human)
Tags6x His tagsExpressionBacterialPromoterT7Available SinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-SP44-sfGFP
Plasmid#163757PurposeExpresses Streptomyces codon-optimised sfGFPDepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialPromoterSP44Available SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMVV099
Plasmid#215305PurposeExpresses sfGFP under induction with the Cuminic Acid Marionette Cassette (CymRAM + PCymRC-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialPromoterPCymRCAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21a - SodA
Plasmid#89612PurposeExpresses recombinant 6HIS tagged T. cruzi superoxide dismutase A protein in E. coli upon IPTG inductionDepositorInsertSuperoxide dismutase A
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only