31,793 results

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pZac2.1 gfaABC1D-LifeAct-GFP

Plasmid

#190199

Purpose

Expresses the small actin binding peptide, LifeAct, with a GFP reporter

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

LifeAct-eGFP

Use

AAV

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCC28

Plasmid

#128284

Purpose

asymmetric plasmid partitioning E. coli - target plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Piq - mRFP, sopC

Expression

Bacterial

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 mouse PARG (439-959)

Plasmid

#132615

Purpose

expresses mouse PARG(439-959) in E. coli.

Depositor

Wenqing Xu

Article

Wang et al PLoS One. 2014 Jan 21;9(1):e86010. doi:

Insert

PARG (439-959)

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pBXNH3CA_MBP

Plasmid

#132700

Purpose

expresses Avi-tagged MBP in E.coli, to use as positive control in ELISA

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Maltose binding Protein

Tags

Avi and His

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET15b-17X-PTDH

Plasmid

#166786

Purpose

Phosphite dehydrogenase for NAD(P)H regeneration

Depositor

Wilfred van der Donk

Article

Yang et al J Am Chem Soc. 2015 Oct 7;137(39):12426-

Insert

Phosphite dehydroenase

Tags

His6

Expression

Bacterial

Mutation

A196R, T201S, A328T, E352N, and C356D- please see…

Promoter

T7

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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His-Tev-UbE2D3

Plasmid

#186805

Purpose

Codon-optimized bacterial expression plasmid. Expresses ubiquitin conjugating enzyme His-Tev-UbE2D3.

Depositor

Lalit Deshmukh

Article

Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

Insert

His-Tev-UbE2D3 (UBE2D3 Human)

Tags

His6-Tev

Expression

Bacterial

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pMAL-SH3(2)-5R

Plasmid

#112089

Purpose

Bacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3(2)-5R (NCK1 Human)

Tags

His6 and MBP

Expression

Bacterial

Mutation

construct contains only repeats of the second SH3…

Promoter

Lac

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pOSV00170

Plasmid

#222934

Purpose

Constitutive GFP expression for Bacillus subtilis

Depositor

Ophelia Venturelli

Article

Cheng et al Nat Commun. 2023 Apr 10;14(1):2001. doi:

Insert

GFP(Sp)

Use

Synthetic Biology

Expression

Bacterial

Promoter

Phyperspank

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pSAM

Plasmid

#45174

Depositor

Jumi Shin

Article

Sathiamoorthy et al PLoS One. 2012;7(10):e47259. doi: 10.137

Type

Empty backbone

Tags

6xHis and 6xHis/Thrombin/T7

Expression

Bacterial

Promoter

T7

Available Since

June 26, 2013

Availability

Academic Institutions and Nonprofits only
EcPhnD90.L1∆∆AD.L297RL301R.pRSET

Plasmid

#33376

Purpose

a single-wavelength phosphonate sensor constructed from E. coli PhnD and cpGFP

Depositor

Loren Looger

Article

Alicea et al J Mol Biol. 2011 Oct 12.

Insert

phnD based Phosphonate Biosensor (phnD E. coli)

Tags

6xHIS

Expression

Bacterial

Mutation

cpGFP146 variant from GCaMP2 inserted in place of…

Promoter

T7

Available Since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pet42c(+)-GST-USP5

Plasmid

#61750

Purpose

express N-terminally GST tagged human USP5

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

USP5 (USP5 Human)

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only
pRAV24

Plasmid

#67716

Purpose

Parental plasmid for transcription of RNAs with a GlmS/M-domain 3'-tag for native purification

Depositor

Robert Batey, Jeffrey Kieft

Article

Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

Insert

Transcription cassette for 3'-tagged RNAs

Expression

Bacterial

Promoter

T7 RNA polymerase

Available Since

Sept. 8, 2015

Availability

Academic Institutions and Nonprofits only
pET His6 ProteinG TEV LIC cloning vector (1P)

Plasmid

#29660

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, Protein G, and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_Olig2

Plasmid

#64842

Purpose

A lentiviral vector for the expression of mouse Olig2 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Olig2 (Olig2 Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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pD434-SR mouse Alpha-synuclein

Plasmid

#89073

Purpose

bacterial expression of mouse Alpha-synuclein

Depositor

Michael J Fox Foundation MJFF

Insert

Alpha-synuclein (Snca Mouse)

Expression

Bacterial

Promoter

T7

Available Since

March 17, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pET-45b-POLR2A-CTD-mCherry

Plasmid

#185011

Purpose

Bacterial Expression of 6xHis C-terminal domain of POLR2A mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

POLR2A (Polr2a Mouse)

Expression

Bacterial

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pRBC-sfGFP-134/150TAG

Plasmid

#174077

Purpose

Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-134/150TAG with C-terminal His6 tag, under T7 promoter

Depositor

Ryan Mehl

Article

Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

Insert

Superfolder GFP

Tags

His-6

Expression

Bacterial

Mutation

D134TAG + N150TAG

Promoter

T7

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-HsSENP1

Plasmid

#71465

Purpose

Encodes human full length SENP1 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 1 (SENP1 Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

Gene optimised for E. coli expression (Genscript)

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pScaf-5544.1

Plasmid

#111405

Purpose

pScaf phagemid with insert for generating DNA origami scaffold 5544 bases in length (v1)

Depositor

Shawn Douglas

Article

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Insert

pScaf-GH'

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT13

Plasmid

#200993

Purpose

pLv1-pUC18R6KT-miniTn7-Tc, TcR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Type

Empty backbone

Expression

Bacterial

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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