32,870 results
-
Plasmid#177310PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_ENO1-Halo
Plasmid#175330PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST-HisMBP-SED1
Plasmid#178191PurposeSED1 biosensor for expression in Escherichia coliDepositorInsertSED1 biosensor
Tags6xHis-MBPExpressionBacterialPromotertacAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2B-56
Plasmid#177925PurposeExpresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoterDepositorArticleInsertOptimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
ExpressionBacterial and YeastMutationV9I; F67S; G72P, F86Y; R171KPromoterMYO2Available SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1TFAKRS
Plasmid#177311PurposetRNA synthetase/tRNA pair for the in vivo incorporation of N6-(trifluoroacetyl)-L-lysine (TFA-Lys) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETSXM2-pLacI-mtrCAB
Plasmid#174618PurposeBacterial expression of gene cluster mtrCAB from Shewanella oneidensis MR-1.DepositorInsertmtrCAB
UseSynthetic BiologyExpressionBacterialPromoterpLacIAvailable SinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS0806 His14-bdSUMO-AwaIscB
Plasmid#176539PurposeBacterial expression of His14-bdSUMO-AwaIscBDepositorInsertAwaIscB
UseCRISPRTags14xHis-bdSUMO tagExpressionBacterialPromoterT7Available SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSR0
Plasmid#161984PurposeAllele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile ribotype 027 and other ribotypes. Uses inducible expression of CD2517.1 toxin as a counter-selection markerDepositorTypeEmpty backboneExpressionBacterialAvailable SinceOct. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
p15a-AsCas12f-apmR
Plasmid#171610PurposeAsCas12f1-based K. pneumoniae genome editingDepositorInsertAsCas12f1
ExpressionBacterialAvailable SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB233
Plasmid#164128PurposePP7-CFP and PhlF-RFP expression plasmidDepositorInsertPP7-CFP
ExpressionBacterialPromoterPtetAvailable SinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB235
Plasmid#164130PurposePhlF-RFP expression plasmidDepositorInsertPhlF-RFP
ExpressionBacterialPromoterPtetAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET24_NusA-Tus
Plasmid#165959PurposeExpression of soluble E. coli Tus proteinDepositorInsertTus
TagsHis-NusA-TevExpressionBacterialPromoterT7Available SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAT218
Plasmid#134886PurposeBacterial expressionDepositorInserteGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBJ4
Plasmid#167133PurposeA kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoterDepositorInsertluxCDABE
UseLuciferaseExpressionBacterialPromoterKanamycin promoterAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBLxVi5
Plasmid#167516PurposeBroad-Host range expression vector, pBBR origin, LuxR activator, PLuxB promoter, Violacein, Gentamicin Resistance Marker.DepositorInsertLuxR-Violacein
UseSynthetic BiologyExpressionBacterialPromoterPLuxBAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbS8c-ddcpf1-Δ
Plasmid#153039PurposeFor CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.DepositorInsertAscpf1
ExpressionBacterialMutationE993AAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
His-TEV-EGFP-PRC1
Plasmid#167540PurposeFor expression of His6-TEV-EGFP-PRC1 in bacteria.DepositorAvailable SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSF-CjPglB-LpxE
Plasmid#128389PurposeExpression of C. jejuni PglB and F. tularensis LpxE in E. coliDepositorInsertsPglB oligosaccharylktransferase
LpxE phosphatase
TagsFlag tagExpressionBacterialPromoterPtac and pBADAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEMPTY::sgRNA2
Plasmid#165459PurposeEscherichia coli – Staphylococcus aureus shuttle vector for plasmid curing in Gram-positive bacteriaDepositorInsertsgRNA2
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterSP01Available SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-EcMscL-TEV-mEGFP
Plasmid#165097PurposeExpresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.DepositorInsertE. Coli Mechanosensitive Channel of Large Conductance (mscL E. coli)
UseSynthetic BiologyTagsmonomeric Enhanced Green Fluorescent ProteinExpressionBacterialPromoterT7Available SinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only