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32,404 results

Showing: 4541 - 4560 of 32404 results

  11. pQhsCDtRNA+

    Plasmid
    #86014
    Purpose
    Multi-gene expression system for purification of human box C/D RNPs with U14-tRNA minigene
    Depositor
    Hong Li
    Article
    Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:
    Inserts
    U14-tRNA chimera (SNORD14A Human)
    15.5K (SNU13 Human)
    fibrillarin (FBL Human)
    NOP56 (NOP56 Human)
    NOP58 (NOP58 Human)
    Use
    Co-expression; purification
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    amino acids 1-401, amino acids 1-411, and amino a…
    Promoter
    LPP and Ptac
    Available Since
    Jan. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86014-plasmid-map-sequence-id-169485

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  12. pST44-AP-1 core

    Plasmid
    #184809
    Purpose
    Bacterial expression of clathrin adaptor AP-1 core complex
    Depositor
    James Hurley
    Article
    Ren et al Cell. 2013 Feb 14;152(4):755-67. doi: 10
    Insert
    Hs AP1S3; Hs AP1B1 (1-584); Mm AP1G1 (1-595); Mm AP1M1
    Tags
    GST tag and His6
    Expression
    Bacterial
    Mutation
    AP1S3: S148C; AP1B1: K359R, E476K
    Promoter
    T7
    Available Since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    184809-plasmid-map-sequence-id-365328

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  14. PPP2R5CA

    Plasmid
    #42369
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PPP2R5CA (PPP2R5C Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. p2B-58

    Plasmid
    #177928
    Purpose
    Expresses yeGFP under control of doxycycline-responsive TET4 promoter (contains four rtTA binding sites)
    Depositor
    Vadim Gladyshev
    Article
    Barre et al (unpublished)
    Insert
    EGFP
    Expression
    Bacterial and Yeast
    Promoter
    TET4 (contains four rtTA binding sites)
    Available Since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177928-plasmid-map-sequence-id-349996

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  19. pEC2935 (p7INT-P23_mNeongreen~ssrA (LDD variant))

    Plasmid
    #218508
    Purpose
    integrative plasmid enabling constitutive expression of a destabilized mNeongreen reporter in Streptococcus pyogenes, targets the attP site of phage T12 in several S. pyogenes strains via an integrase
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    mNeongreen
    Tags
    modified ssrA degradation tag
    Expression
    Bacterial
    Mutation
    deletion of MCS containing Plac_lacZa, introducti…
    Promoter
    P23
    Available Since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    218508-plasmid-map-sequence-id-438746

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  20. pDNA2.0 6H-TEV-KRAS G13D

    Plasmid
    #111851
    Purpose
    Expresses activated human KRAS G13D (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.
    Depositor
    Kenneth Westover
    Article
    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.
    Insert
    KRAS G13D (KRAS Human)
    Tags
    6xHis-tag and TEV protease cleavage sequence
    Expression
    Bacterial
    Mutation
    Changed Glycine 13 to Aspartic Acid
    Promoter
    T5
    Available Since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111851-plasmid-map-sequence-id-213465

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Showing: 4541 - 4560 of 32404 results