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Plasmid#173834PurposeExpresses 80 kD Penn State ladder proteinDepositorInsertSTRHSTPABPACIL1bRCC1
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pV1F
Plasmid#205084PurposeProkaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.DepositorInsertAtVIP1
UseTagsExpressionBacterialMutationPromoteragorbacterium virB operon promoterAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT-Hrp48-ADARcd-E488Q-V5
Plasmid#139685PurposeExpresses the hyperactive form of the catalytic domain of the ADAR protein fused to HRP-48 for use in "TRIBE" assaysDepositorInsertHrp48, ADAR (Adar Fly)
UseTagsV5ExpressionBacterial and InsectMutationPromoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ2-Pdadh-LacZ
Plasmid#191618PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine dehydroxylase promoter-lacZDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDUET-1-alpha-synuclein-mCerulean3-His6
Plasmid#110061PurposeFor bacterial expression of human alpha-synuclein, carboxyl terminally tagged with mCerulean3 and poly-histidinesDepositorInsertalpha-synuclein (SNCA Human)
UseTags6 x His and mCerulean3ExpressionBacterialMutationPromoterT7Available sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-26b-Nb127D01-HA-His
Plasmid#171566PurposeBacterial expression of HA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-HA-His)DepositorInsertNb127D01-HA-His (CXCR2 Human)
UseTagsHA-tag/His-tag and PelB signal peptideExpressionBacterialMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
TDP-43_CTD_A321V
Plasmid#98671PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with A321VDepositorInsertTDP-43 (TARDBP Human)
UseTags6xHisExpressionBacterialMutationA321VPromoterAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET303-ColE1-T-E192C
Plasmid#180233PurposeExpresses T domain of Colicin E1 (residues 1-190) with C terminal cysteine for maleimide chemistryDepositorInsertColicin E1
UseTags6x His-tagExpressionBacterialMutationTruncation of ColE1 containing T domains (residueā¦PromoterT7Available sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLEVI(408)-ColE-iLight-msfGFP
Plasmid#170268PurposeExpresses LexA_DBD-iLight-msfGFP under J23116 promoter and mCherry under ColE promoter with a LexA408 operator in bacteria.DepositorInsertiLight
UseTagsmsfGFPExpressionBacterialMutationTo design iLight for expression in bacteria, a fuā¦PromoterpJ23116Available sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
RBD#3L
Plasmid#193020PurposehnRNP A1 RRM2 RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13aDepositorInsertCas13a
UseTagshnRNP A1 RRM2ExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIB184-Km
Plasmid#90195PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoterDepositorTypeEmpty backboneUseTagsNoneExpressionBacterialMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
hSDH5_G78R
Plasmid#113047PurposeExpression of mutant hSDH5DepositorInserthSDH5_G78R (SDHAF2 Human)
UseTagsExpressionBacterialMutationG78R, Insert begins at amino acid 39PromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_His-CBD-SpEndoH
Plasmid#218196PurposeBacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoHDepositorInsertEndoH
UseTagstufA-His-CBDExpressionBacterialMutationcodon optimized Ecoli, residues 45-313PromoterT7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCBP1_Full-length_pGEX
Plasmid#135108PurposeRecombinant expression of PCBP1 with dual-affinity tagDepositorInsertPCBP1 (PCBP1 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTarget12f1
Plasmid#171183PurposeConstitutive expression of sgRNA without donor DNADepositorInsertsgRNA scaffold in the CRISPR/Cas12f1 system
UseCRISPRTagsExpressionBacterialMutationPromoterJ23119(SpeI) promoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPP076
Plasmid#158794PurposepRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-1 in MCS1 for expression in E. coli and protein purificationDepositorInsertCasPhi-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pLib6.4
Plasmid#133783PurposeLibrary backboneDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJL1-mCherry
Plasmid#102629PurposeIn vitro expression of mCherry from the T7 promoterDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL0284 (pQCascade_entry)
Plasmid#130635PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array contains two BsaI sites for spacer cloning.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only