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PZac2.1 hSyn1-BioID2-BioID2-HA

Plasmid

#176855

Purpose

Expresses the promiscuous small biotin ligase, BioID2, in neurons

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

hSyn1

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1-HiBiT

Plasmid

#198310

Purpose

Loads HiBiT-tagged Pdp1 into PVCs; for payload loading assays; also contains Pnf

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-CD11bDARPin

Plasmid

#198295

Purpose

Produces PVCs retargeted with anti-CD11b DARPin F7

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-CD11bDARPin

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-truncated

Plasmid

#198286

Purpose

Contains a tail fiber with a truncated binding domain (lacking aa403-476 in Pvc13); produces nontargeting PVCs

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-truncated

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_FLAG-pvc2

Plasmid

#198285

Purpose

Contains an external epitope tag on the sheath protein; for immunofluorescence-based binding assays

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_FLAG-pvc2

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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His-Tev-Nedd4L

Plasmid

#186806

Purpose

Codon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-Nedd4L.

Depositor

Lalit Deshmukh

Article

Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

Insert

His-Tev-Nedd4L (NEDD4L Human)

Tags

His6-Tev

Expression

Bacterial

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pSPIH6

Plasmid

#190676

Purpose

E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)

Depositor

John Vederas

Article

Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi

Inserts

Small ubiquitin-like modifier
Mxe GyrA intein with C-terminal chitin binding domain and His tag

Tags

Chitin binding domain, His tag, and N-terminal Hi…

Expression

Bacterial

Mutation

Added C-terminal his tag and None

Promoter

T7

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pES002 MBP-GSK3β_S9A-HA-His

Plasmid

#196184

Purpose

Expresses MBP-GSK3β_S9A-HA-His (human Glycogen Synthase Kinase 3β S9A mutant as a fusion protein with MBP, HA, and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

Insert

GSK3B (GSK3B Human)

Tags

HA, His6, and MBP

Expression

Bacterial

Mutation

changed Serine 9 to Alanine

Promoter

Tac

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pλPR cI-Elysis

Plasmid

#176891

Purpose

Bacterial expression plasmids for production of E. coli bacterial ghost (BG) capsules

Depositor

James Inglese

Article

Dranchak et al Dis Model Mech. 2023 Mar 1;16(3):dmm0498

Insert

Lysis E gene from PhiX174 and lambda PR-cI857 regulatory system

Expression

Bacterial

Promoter

T7

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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B-gTEMP/pRSET-b

Plasmid

#188446

Purpose

B-gTEMP for bacterial expression

Depositor

Takeharu Nagai

Article

Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

Insert

B-gTEMP

Tags

6xHis-tag

Expression

Bacterial

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pRha-ABE7.10-SpCas9-NG

Plasmid

#170663

Purpose

Expresses the base editor ABE7.10-SpCas9-NG in bacterial cells

Depositor

David Liu

Article

Krishnamurthy et al Nucleic Acids Res. 2021 Oct 11;49(18):10

Insert

ABE7.10-SpCas9-NG

Tags

8xHIS and BP-NLS

Expression

Bacterial

Promoter

pRhaBAD

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pRT498-3xOtUBD

Plasmid

#190088

Purpose

Expresses 3 tandem OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

Depositor

Mark Hochstrasser

Article

Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

Insert

3xOtUBD

Tags

6xHis and MBP

Expression

Bacterial

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBA615

Plasmid

#186239

Purpose

eLbuCas13a Negative Control (RFP targeting)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

pTet-eLbuCas13a + crRNA negative control

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAP05

Plasmid

#186261

Purpose

sfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoter

Depositor

Yongku Cho

Article

Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

Inserts

sfGFP
EL222 (ELI_04755 Erythrobacter litoralis)

Expression

Bacterial

Promoter

BBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAB150

Plasmid

#101661

Purpose

Opto-T7RNAP*(563)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAF-HdrB3

Plasmid

#184908

Purpose

carrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferridurans

Depositor

Radhakrishnan Mahadevan

Article

Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

Insert

Cas9-sgRNA-homology arms

Use

CRISPR; Broad-host-range

Expression

Bacterial

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pMC_MS2_DNA_VLP

Plasmid

#179156

Purpose

Expression of maturation protein and his-tagged coat protein dimer from MS2 phage

Depositor

Paul Freemont

Article

Crone et al GEN Biotechnol. 2022 Dec 1;1(6):496-503.

Inserts

Maturation Protein
Coat Protein Dimer

Expression

Bacterial

Promoter

T7

Available Since

Sept. 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pQE80L Hu IL-15-His tag

Plasmid

#183256

Purpose

Bacterial expression of human IL-15 with N-terminal His tag

Depositor

Alexander McLellan

Insert

IL-15 (IL15 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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Integrin Beta1-mPA-GFP

Plasmid

#186302

Purpose

Expresses mPA-GFP tagged Integrin beta-1 in mammalian cells on a CMV promoter

Depositor

Pakorn Kanchanawong

Article

Jain et al Nat Protoc. 2022 Jul 27. pii: 10.1038/s4

Insert

Integrin Beta 1 (ITGB1 Human)

Tags

mPA-GFP

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pKL2315

Plasmid

#187876

Purpose

Expresses Cre recombinase.

Depositor

Kan Wang

Article

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Insert

Cre recombinase

Use

Cre/Lox

Expression

Bacterial

Promoter

J23107

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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