30,803 results
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Plasmid#190793PurposeTemplate vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.DepositorInsertsgRNA (dummy)
UseSynthetic BiologyExpressionBacterialPromoterBBa_J23117Available SinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACRISPR-KM
Plasmid#203587PurposeA sgRNA expression plasmid for genome editing in BurkholderiaDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_BauA
Plasmid#128952Purposeexpresses BauA in E.coliDepositorInsertferric acinetobactin receptor
TagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-66)PromoterT7Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCCL-GKL1RC-Short-LEUg1mKateg2GKL2ORF10UCS
Plasmid#52345PurposeIntegration cassette to create p1-Short-Leu-mKate with p2 ORF10 UCS>mKateDepositorInsertmKate2
UseP1 integration vectorExpressionBacterialMutationchanged V2HPromoterpGKL2 ORF10 UCSAvailable SinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
PET-28a-YTH domain of YTHDF1
Plasmid#228775PurposeProtein expression plasmid for YTH domain of YTHDF1 in E.Coli.DepositorAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET32bplus_trxAChEwt
Plasmid#83916PurposeEncodes an E. coli codon optimized version of wild type human acetylcholinesteraseDepositorInsertShort form of hAChE, codon optimised for E.coli expression (ACHE Human, Synthetic)
TagsHis, S-tag, and TRXExpressionBacterialMutationcodon optimised for E.coli expressionPromoterT7 promoterAvailable SinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET24a-AlkB deltaN11
Plasmid#73622PurposeExpresses E. coli AlkB absent the first 11 amino acids and with a C-terminal histidine tagDepositorInsertAlkB (alkB E. coli)
TagsHisExpressionBacterialMutationdeletion of first 11 AAPromoterT7Available SinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTXBI-mclover-YTHDC1-FL
Plasmid#177121PurposeBacterial expression of Full-length YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purificationDepositorInsertYTHDC1 (YTHDC1 Human)
TagsMxe GyrA intein-Chitin binding domain for protein…ExpressionBacterialPromoterT7Available SinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pelBHisTEV
Plasmid#157741Purposeempty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sitesDepositorTypeEmpty backboneTagsPelB signal sequence + His10-tag + TEV protease c…ExpressionBacterialPromoterT7lacAvailable SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pccGFPG83I
Plasmid#73650PurposeFluorescent reporter for transmembrane protein dimerization negative control.DepositorInsertglycophorin A- G83I
ExpressionBacterialMutationglycine 83 to isoleucineAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE-hTR
Plasmid#21811DepositorAvailable SinceSept. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
LIC1B_Caspase-LOVN7C-7
Plasmid#104629Purposebacterial expression of light-activated caspase-3 with N7C-7 linker variationDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 aph(3'')-Ia
Plasmid#112879PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYSD085
Plasmid#212922PurposeEncodes the HA-tagged 649 Stalk yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsert649 Stalk
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 aph(9)-Ia
Plasmid#112882PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertaph(9)-Ia
Tags6xHisExpressionBacterialPromoterPblaAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-SpG-His
Plasmid#179318PurposePlasmid for bacterial expression and purification of SpG-Cas9DepositorInsertHuman codon-optimized Cas9 variant SpG
UseCRISPRTags6xHis and SV40-NLSExpressionBacterialMutationSpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337RAvailable SinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRN3P-NLS-EGFP-Btgh-3NLS
Plasmid#194469PurposeEncodes for bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.DepositorInsertBtGH84
UseFor in vitro transcription from t3 promoter after…TagsSV40 Nuclear Localization Signal, SV40 Nuclear Lo…ExpressionBacterialAvailable SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLG029 (mzaABCDE)
Plasmid#157907PurposeExpresses the mzaABCDE defense systemDepositorInsertmzaABCDE (5-gene system containing a MutL homolog)
ExpressionBacterialAvailable SinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG030 (TerY-P)
Plasmid#157908PurposeExpresses the TerY-P defense systemDepositorInsertTerY-P triad (vWA + kinase + phosphatase)
ExpressionBacterialAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-TFIIE
Plasmid#171083PurposeCo-expresses human TFIIE. The resulting plasmid was used to generate a single expression construct encoding 2 subunits, with His-tag on TF2E1.Originally from MN Gonzalez, was submitted with permissionDepositorTagsHisExpressionBacterialPromoterT7 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only