30,307 results

1. T7 promoter + filler DNA reporter plasmid

   Plasmid

   #156454

   Purpose

   A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

   Inserts

   NeoR/KanR
   TcR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   ATG start codon mutated to ACG

   Promoter

   Em7 and tet

   Available since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-GST-His6-1_HBD

   Plasmid

   #171869

   Purpose

   Expresses a specific DNA-RNA hybrid sensor protein with the HBD domain of RNase H1 in bacterial cells

   Depositor

   Kaiwei Liang

   Article

   Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

   Insert

   Hybrid binding domain of Rnase H1
   

   Use

   Tags

   GST- and His10-tag

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQTEV-MAT2B

   Plasmid

   #34795

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   methionine adenosyltransferase II, beta (MAT2B Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJV62

   Plasmid

   #26910

   Depositor

   Graham Hatfull

   Article

   van Kessel et al Mol Microbiol. 2008 Mar . 67(5):1094-107

   Insert

   Che9c gene 61
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSALECT-EcoBam

   Plasmid

   #59705

   Purpose

   Folding reporter with ampicillin resistance as a readout

   Depositor

   Christopher Anderson

   Article

   Hsiau et al PLoS One. 2015 Mar 19;10(3):e0119927. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. AviTag-SpyCatcher

   Plasmid

   #72326

   Purpose

   Bacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.

   Depositor

   Mark Howarth

   Article

   Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

   Insert

   AviTag-SpyCatcher
   

   Use

   Tags

   N-term His6 tag and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBJ4

   Plasmid

   #167133

   Purpose

   A kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

   Depositor

   Akira Mine

   Article

   Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

   Insert

   luxCDABE
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Kanamycin promoter

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBroad-T7RNAP-apraR

   Plasmid

   #199993

   Purpose

   T7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) apraA

   Depositor

   Farren Isaacs

   Article

   Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

   Insert

   T7RNAP Circuit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   copFnull

   Promoter

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD_His B_QuasAr2

   Plasmid

   #64134

   Purpose

   Fluorescent reporter of membrane voltage with improved sensitivity

   Depositor

   Adam Cohen

   Article

   Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

   Insert

   QuasAr2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ki67∆FHA-mNeonGreen-IRESpuro2

    Plasmid

    #183739

    Purpose

    Expresses Ki67∆FHA-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67_lacking FHA (MKI67 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    MKI67(lacking FHA)

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pO_72

    Plasmid

    #78235

    Purpose

    Transcripts the r_oligo part that includes the HIV reverse transcriptase binding site (HTBS)

    Depositor

    Christopher Voigt

    Article

    Elbaz et al Nat Commun. 2016 Apr 19;7:11179. doi: 10

    Insert

    r_oligo
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    j23100

    Available since

    Aug. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMal-E6LLO

    Plasmid

    #51281

    Purpose

    Expresses a fibronectin fusion with listeriolysin targeting EGFR in bacteria

    Depositor

    Dane Wittrup

    Article

    Pirie et al Mol Cancer Ther. 2013 Sep;12(9):1774-82.

    Insert

    E6LLO
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. CJ8421_04975 Rptr2 and CJ8421_04975 mRNA

    Plasmid

    #194210

    Purpose

    Expresses the Campylobacter mRNA CJ8421_04975 and the mRNA-targeting reprogrammed tracrRNA 2

    Depositor

    Chase Beisel

    Article

    Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

    Insert

    CJ8421_04975 mRNA and CJ8421_04975 Rptr2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. PRDM1

    Plasmid

    #25249

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PRDM1_04 (PRDM1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

15. A1-LCD deltahexa

    Plasmid

    #169714

    Purpose

    Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, hexapeptide including aa 259-264 deleted

    Depositor

    Tanja Mittag

    Article

    Martin et al Science. 2020 Feb 7;367(6478):694-699. d

    Insert

    hnRNPA1 (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    hnRNPA1-A Low complexity domain including amino a…

    Promoter

    T7

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-SpySrtA

    Plasmid

    #51139

    Purpose

    expression plasmid for N-terminal his tagged S.pyogenes SrtA

    Depositor

    Hidde Ploegh

    Article

    Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

    Insert

    S.pyogenes SrtA
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-80

    Promoter

    T7

    Available since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-puro-TagBFP-β-ACTIN-3′UTR-8xMS2

    Plasmid

    #205160

    Purpose

    express β-ACTIN-3′UTR-8xMS2

    Depositor

    Weirui Ma

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    β-ACTIN-3′UTR-24xMS2
    

    Use

    Tags

    tagBFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pP2-phiV10

    Plasmid

    #196333

    Purpose

    Plasmid expressing chimeric P2-phiV10 tail fiber

    Depositor

    Baojun Wang

    Article

    Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

    Insert

    chimeric tail fiber P2-phiV10
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P2 late promoter

    Available since

    June 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDonor_Tn-pOUT

    Plasmid

    #213905

    Purpose

    Mariner transposon with outward-facing pJ23104 promoter

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    mariner transposon with kan resistance and outward-facine pJ23104 promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMA7-SacB

    Plasmid

    #79968

    Purpose

    TM-MAGE strain

    Depositor

    Morten Sommer

    Article

    Lennen et al Nucleic Acids Res. 2016 Feb 29;44(4):e36

    Inserts

    Lambda Red recombinase beta subunit
    DNA adenine methylase
    Levansucrase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Sept. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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