31,059 results

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pEGFP_PCNA_IRES_puro2b

Plasmid

#21048

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

hPCNA

Tags

GFP

Expression

Bacterial and Mammalian

Available Since

May 15, 2009

Availability

Academic Institutions and Nonprofits only

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WT C-His Ana GvpC in pET28a

Plasmid

#85732

Purpose

Expresses His-tagged GvpC from Anabaena flos-aquae in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

Insert

Gas vesicle protein C (his-tagged)

Tags

His-tag

Expression

Bacterial

Mutation

Added an extra glycine after the methionine at th…

Promoter

T7

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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6972 pPROEX-HTb-SSA1

Plasmid

#1231

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Ssa1 (SSA1 Budding Yeast)

Tags

his tag (tev cleavable)

Expression

Bacterial

Available Since

Aug. 9, 2005

Availability

Academic Institutions and Nonprofits only
pAM5406

Plasmid

#132661

Purpose

RSF1010-based broad host-range plasmid with additional RK2 bom site and pUC origin

Depositor

Susan Golden

Article

Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pEvoltac-EcW-TGA-h14

Plasmid

#218764

Purpose

encodes EcWRS-h14 and EcW-tRNA-TGA

Depositor

Abhishek Chatterjee

Article

Italia et al Nat Chem Biol. 2017 Apr;13(4):446-450. d

Insert

EcWRS-h14

Expression

Bacterial

Mutation

S8A, V144G, V146C

Promoter

tacI

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTOX10

Plasmid

#127455

Purpose

Encodes YhaV toxin. Gent-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Insert

yhaV toxin

Expression

Bacterial

Promoter

pRha

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pET-X(4130)B1

Plasmid

#168018

Purpose

Expresses X(4130)B1-6xHis in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

X(4130)B1-6xHis

Tags

Hisx6

Expression

Bacterial

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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OPEN-HLA-B*07:02-BirA

Plasmid

#239752

Purpose

E. coli expression of BirA tagged HLA-B*07:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-B*07:02 (HLA-B Human)

Tags

BirA substrate peptide

Expression

Bacterial

Mutation

G120C

Promoter

T7

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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OPEN-A*24:02

Plasmid

#235133

Purpose

E. coli expression of HLA-A*24:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-A*24:02 (HLA-A Human)

Expression

Bacterial

Mutation

G120C

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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OPEN-HLA-A*01:01

Plasmid

#235134

Purpose

E. coli expression of HLA-A*01:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-A*01:01 (HLA-A Human)

Expression

Bacterial

Mutation

G120C

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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OPEN-HLA-A*11:01-BirA

Plasmid

#235135

Purpose

E. coli expression of BirA tagged HLA-A*11:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-A*11:01 (HLA-A Human)

Tags

BirA

Expression

Bacterial

Mutation

G120C

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pJS119

Plasmid

#207592

Purpose

GFP-TonB deltaII expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-TonB deltaII

Expression

Bacterial

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pJS104

Plasmid

#207589

Purpose

GFP-TolA deltaII expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-TolA deltaII

Expression

Bacterial

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pJS70

Plasmid

#207586

Purpose

GFP-TolA H22A expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-TolA H22A

Expression

Bacterial

Mutation

H22A

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528-PPxY1-GS-WW3e

Plasmid

#166705

Purpose

expresses an N-terminal fusion of AMOT PPxY1 to NEDD4L WW3 in bacterial cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

AMOT PPxY1 fused to NEDD4L WW3

Tags

His-SUMO

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pUG-302

Plasmid

#104731

Purpose

Level 2 construct

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

HIS-Ubq_UBA1_HA-UBC11_EL3

Tags

HIS-Ubq, HA-E2

Expression

Bacterial

Promoter

T7

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ3538 eforRed-targeting sgRNA

Plasmid

#239455

Purpose

guide for eforRed

Depositor

Stanley Qi

Article

Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

Insert

guide targeting eforRed

Use

CRISPR; Cell-free system using bacterial extract

Expression

Bacterial

Promoter

J23119

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pWZ699

Plasmid

#208117

Purpose

Payload yeast assembly vector with TK-mActb on backbone

Depositor

Jef Boeke

Article

Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

Type

Empty backbone

Use

Synthetic Biology; Copy number inducible

Expression

Bacterial, Mammalian, and Y…

Promoter

Human PGK1 for thymidine kinase

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET41a iGluu

Plasmid

#119834

Purpose

Production of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluu

Tags

GST

Expression

Bacterial

Mutation

S72T

Promoter

T7

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pST-2K

Plasmid

#44564

Purpose

for ectopic constitutive expression of two mycobacterial proteins in one vector

Depositor

Vinay Nandicoori

Article

Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

Type

Empty backbone

Use

E.coli-mycobacteria shuttle vector

Tags

Flag tag and His tag

Expression

Bacterial

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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