31,793 results
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Plasmid#222872PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 5-Cyano-L-Tryptophan (5-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-TEV-SNAP-PRC1
Plasmid#167541PurposeFor expression of His6-TEV-SNAP-PRC1 in bacteria.DepositorAvailable SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hisx6-Sirt6
Plasmid#20277DepositorAvailable SinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMP2463
Plasmid#107774PurposeExpression of EGFPDepositorInsertpLacZ-EGFP in pBBR1-mcs5
TagspLacZ-EGFPExpressionBacterialPromoterpLacZAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFCiR2
Plasmid#167512PurposeBroad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, mRFP, Kanamycin Resistance Marker.DepositorInsertCinRAM-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPCinAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKEW303-AcrVA5
Plasmid#115657PurposeExpresses AcrVA5 in bacteria, under TetR control.DepositorInsertAcrVA5
UseSynthetic BiologyExpressionBacterialPromoterPtetAvailable SinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX:PGRMC1
Plasmid#136062PurposeBacterial expression of pGEX:PGRMC1 to perform a GST-pulldown assay and identify new proteins that interact with GST-PGRMC1 in the human endometrial stroll cells.DepositorInsertProgesterone receptor membrane component 1 (PGRMC1 Human)
TagsGST-PGRMC1ExpressionBacterialAvailable SinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCURE
Plasmid#23013DepositorInsertI-CreI homing endonuclease
ExpressionBacterialMutationI-CreI expression is controlled by the arabinose …Available SinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pETSXM2-pLacI-mtrC
Plasmid#174615PurposeBacterial expression of mtrC from Shewanella oneidensis MR-1.DepositorInsertMtrC
UseSynthetic Biology; Shuttle vector in e. coli and …ExpressionBacterialPromoterpLacIAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pK27Sumo_His-SUMO-nsp7 (SARS-CoV-2)
Plasmid#169186PurposeTo express His-SUMO-nsp7 (SARS-CoV-2) in E. coliDepositorInsert14His-SUMO-nsp7 (ORF1ab SARS-CoV-2)
Tags14His-SUMO (Ulp1-cleavable)ExpressionBacterialMutationCodon optimised for E. coliPromoterT5Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMON-HsNMT1
Plasmid#67475PurposeExpression of human N-myristoyltransferase 1 for N-myristoylation of ARF family proteins in bacteriaDepositorInsertHsNMT1 (NMT1 Human)
ExpressionBacterialAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
ABL1_HUMAN_D0
Plasmid#79727PurposeThis plasmid encodes the kinase domain of ABL1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGE144 (MutaEco; aTC-induced)
Plasmid#189824PurposeExpresses MutaEco for generating mutations in the E. coli genome (aTC-induced)DepositorInsertEcoRNAPα-PmCDA1
ExpressionBacterialPromotertetR/tetA promoterAvailable SinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZA16mflon
Plasmid#75439PurposePlasmid for arabinose-induced expression of mf-lon. Note that this plasmid contains the original mf-lon cassette that is not codon optimized for expression in E. coli.DepositorInsertmf-lon
ExpressionBacterialPromoterpBADAvailable SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS
Plasmid#89508PurposeExpression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells.DepositorInsertrAPOBEC1-nCas9 (D10A)-UGI-NLS
TagsUGI and rAPOBEC-XTENExpressionBacterialMutationD10A mutation of Cas9PromoterT7Available SinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMG051 MBP-β-catenin-His, GST-CK1α
Plasmid#154072PurposeCo-expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) and GST_CK1α in E.coli to produce primed MBP-β-catenin-HisDepositorTagsGST, His6, and MBPExpressionBacterialPromoterTacAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30b_OmpAT7Sav
Plasmid#138589PurposeT7-tagged streptavidin with native C-terminus and periplasmic export signal OmpA; controlled by PT7 promoter; KanR, pBR322 oriDepositorInsertT7-tagged streptavidin variant with native C-terminus and OmpA signal peptide for periplasmic export
UseSynthetic BiologyTagsOmpA export signal and T7 tagExpressionBacterialPromoterPT7Available SinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
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PZac2.1 hSyn1-BioID2-BioID2-HA
Plasmid#176855PurposeExpresses the promiscuous small biotin ligase, BioID2, in neuronsDepositorInsertBioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialPromoterhSyn1Available SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)/MBP-His-PINK1
Plasmid#38005DepositorAvailable SinceMarch 10, 2014AvailabilityAcademic Institutions and Nonprofits only