33,449 results
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Plasmid#159579PurposeE. coli protein expression of His6-MBP-tev-Hs.NF1 DEF (1198-1530)DepositorAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only
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pET-45b-mCherry
Plasmid#145279PurposeBacterial Expression of 6xHis mCherry Fusion ProteinDepositorInsertmCherry
ExpressionBacterialAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
LaM8-AK74
Plasmid#159594PurposeOpto-NanobodyDepositorInsertLaM8 with AsLOV2 at AK74
ExpressionBacterialAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-GFP-C-LACE
Plasmid#133913PurposeBacterial expression of His6-GFP-C-LACEDepositorInsertsuperfolder green fluorescent protein
TagsLACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…ExpressionBacterialPromoterT7Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pADsacA
Plasmid#158649PurposeConstitutive transcription of sacA crRNA and GFPDepositorInsertsacA crRNA
UseCRISPR and Synthetic Biology; Shuttle vector baci…ExpressionBacterialPromoterPvegAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAT03-Apr
Plasmid#154756PurposeFor curing of the resistance marker of RecET recombinase system in Acinetobacter baumannii, AprarDepositorInsertFLP recombinase
ExpressionBacterialAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
P22_wtCP_SP141his
Plasmid#122651PurposeExpression of P22 virus like particle in bacteriaDepositorInsertP22 Coat: WT / SP: 6xHis-SP141
ExpressionBacterialMutationtruncated SP, P120H in the capsid scaffold (see …Available sinceAug. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL715
Plasmid#140385PurposeExpresses MphR protein under the control of T7-lacO promoterDepositorInsertT7-lacO-MphR-T
Tags6XHisExpressionBacterialAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDonor-GDH
Plasmid#140629PurposeEncodes a mini-transposon derived from V. cholerae Tn6677 and T7-GDH cargo gene.DepositorInsertMini-Tn6677, T7lac-GDH gene cassette
UseCRISPR; TransposonExpressionBacterialAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJBL723
Plasmid#140393PurposeExpresses HucR protein under the control of T7-lacO promoterDepositorInsertT7-lacO-HucR-T
Tags6XHisExpressionBacterialAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF_LuxR
Plasmid#140039PurposeConstutitive expression of LuxR protein.DepositorInsertpLuxL and LuxR
UseSynthetic BiologyExpressionBacterialAvailable sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL0M-S-mNeonGreen-EC18153
Plasmid#137075PurposeGolden Gate Level 0 S module for N-terminal fluorescent protein taggingDepositorInsertmNeonGreen DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
UseSynthetic BiologyExpressionBacterialAvailable sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGBW-m4046754
Plasmid#145647PurposeBacterial Expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046814
Plasmid#145601PurposeBacterial Expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
p_SNIPR_sensor_GFP
Plasmid#139463PurposeT7 RNAP-driven expression of SNIPR RNA with a GFP-ASV reporterDepositorInsertSNIPR_GFP
ExpressionBacterialAvailable sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Citroff1
Plasmid#134301PurposeBacterial expression of inverse-response citrate sensor with high binding affinityDepositorInsertCitroff1
Tags6-His tagExpressionBacterialPromoterpBADAvailable sinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOPS0381
Plasmid#133231PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter PsNifH (pOGG043), sfGFP (pOGG037) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSiMPlk_N + pSiMPlk_C
Plasmid#134310PurposeMaintenance of 2 plasmids with a single antibiotic in E. coliDepositorInsertkanR_gp41-1 N-intein + kanR_gp41-1 C-intein
ExpressionBacterialAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSHDY
Plasmid#137661PurposeConjugative vector with streamlined cloning capabilities. Contains two cloning sites for modular insertion of genetic parts.DepositorTypeEmpty backboneExpressionBacterialAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-Duet-1-NONO(53-312)
Plasmid#135442PurposeExpresses NONO 53-312 in the second site; for co-expression with His-tagged proteinDepositorInsertNon-POU domain-containing octamer-binding protein (NONO Human)
ExpressionBacterialPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only