32,926 results
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Plasmid#224362PurposeExpress AlaRS protein for his-tagged purificationDepositorAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only
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PT7lacO-His6-NDK
Plasmid#224376PurposeExpress NDK protein for his-tagged purificationDepositorAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAurora2
Plasmid#213134PurposeIntegrated transcriptional and translational control for stringently blue-light-activated gene expressionDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(4/5FTrp)RS
Plasmid#207620PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 4-Fluoro-L-Tryptophan (4FTrp) and 5-Fluoro-L-Tryptophan (5FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGD335
Plasmid#219499PurposepAGD248 derivative with the retron Eco1 msr, sfGFP flanked by 2 BsaI sites , and the rest of the retron recombineering operon (Eco1 RT, CspRect, MutL-E32K) downstream of the pBad promoterDepositorInsertEco1 msr, sfGFP, Eco1 RT, CspRecT, MutLE32K
ExpressionBacterialPromoterpBADAvailable SinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYHY3
Plasmid#216109PurposeaTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.DepositorInsertNanoLuc
UseSynthetic Biology; E. coli-bacteroides shuttle ve…Tags3xFLAG tag and BT3630 signal peptideExpressionBacterialPromoterP1TDP-A21Available SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMP11
Plasmid#215559PurposepKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 originDepositorInsertsCas9
gRNA targeting pBR322 origin of replication
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23105 and tetAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-DepoPETase
Plasmid#218702PurposeEncodes codon optimized DepoPETase enzyme for bacterial expressionDepositorInsertDepoPETase
Tags10xHisExpressionBacterialAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(6/7FTrp)RS
Plasmid#207621PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 6-Fluoro-L-Tryptophan (6FTrp) and 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT11.R
Plasmid#201003PurposepLv1-pUC18R6KT-miniTn7-Gm-mCherry, GmR, ApRDepositorInsertmCherry
ExpressionBacterialPromoterJ23104Available SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMApra-Ptac
Plasmid#218357PurposeEmpty vector with lacIq-Ptac expression systemDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_His-CBD-SpEndoH
Plasmid#218196PurposeBacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoHDepositorInsertEndoH
TagstufA-His-CBDExpressionBacterialMutationcodon optimized Ecoli, residues 45-313PromoterT7Available SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
OmpF
Plasmid#213135PurposeExpresses outer membrane protein FDepositorAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b-His6-UBCH7
Plasmid#212711PurposeBacterial expression of His6-UBCH7DepositorInsertUBCH7
Tags6xHisExpressionBacterialAvailable SinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28sumo-MDA5-Full-length
Plasmid#210826Purposeprotein expression in ecoliDepositorAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMW41: pET-His-MBP-SUMO-R2Bm-SII
Plasmid#209290PurposeExpresses the R2Bm ORF in E. coliDepositorInsertR2
TagsHis, MBP, StrepII, and bdSUMOExpressionBacterialPromoterT7Available SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPIPL12-FnCas12a
Plasmid#199563PurposeTetracycline-Inducible Fncas12a gene expression in E. coli and clostridiaDepositorInsertFncas12a
UseCRISPR and Synthetic Biology; Tetracycline induci…ExpressionBacterialPromoterCOtetR-PIPL12Available SinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPfetFn-Target_v2
Plasmid#199564PurposegRNA expression for FnCas12a in E. coli and clostridiaDepositorInsertSacB
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPthl_fUAvailable SinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pR562
Plasmid#207886PurposeCreation of Level 0 entry clonesDepositorInsertamilCP
ExpressionBacterialAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1_WT RAD23B
Plasmid#201457PurposeExpresses human RAD23B with an N-terminal GST tag in E. coliDepositorInsertUV excision repair protein RAD23 homolog B (RAD23B Human)
TagsGSTExpressionBacterialMutationCoding sequence has been optimised for expression…Promotertac promoterAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only