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pGex-4T-2_GABARAP

Plasmid

#73948

Purpose

For recombinant expression of human GABARAP in E.coli

Depositor

Dieter Willbold

Article

Stangler et al J Biomol NMR. 2001 Oct;21(2):183-4.

Insert

GABARAP (GABARAP Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
pQE30-HyPerFLEX

Plasmid

#217569

Purpose

Bacterial expression of HyPerFLEX

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX

Tags

6xHis

Expression

Bacterial

Promoter

T5

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pTW335p3

Plasmid

#237329

Purpose

expresses Z. mays RbcL RbcS-6xHis

Depositor

Tina Wang

Article

Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

Inserts

Z. mays RbcL
Z. mays RbcS-6xHis

Tags

6xHis and None

Expression

Bacterial

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET24a_SMOX

Plasmid

#186345

Purpose

C-terminal His-Tag and a TEV cleaveage site, to transform into E. coli BL21 (DE3) cells

Depositor

Sandra Gabelli

Article

Dunston et al Acta Naturae. 2020 Jul-Sep;12(3):140-144

Insert

Spermine oxidase (SMOX Human)

Tags

His tag

Expression

Bacterial

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pJUMP29-1A(lacZ)

Plasmid

#126978

Purpose

Level 1 vector. With lacZ as alternative cloning reporter. OriV 9 (pBBR322/ROP; medium copy number).

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pGex-Cav1

Plasmid

#33364

Depositor

Ambra Pozzi

Article

Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

Insert

Cav1 (CAV1 Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pBAD/HisD-PATagRFP

Plasmid

#31945

Depositor

Vladislav Verkhusha

Article

Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

Insert

PATagRFP

Tags

His

Expression

Bacterial

Mutation

R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

Promoter

araB

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pSb_init containing anti SARS-CoV-2_RBD sybody Sb#45

Plasmid

#153526

Purpose

Expression of the anti SARS-CoV-2_RBD sybody Sb#45

Depositor

Markus Seeger

Article

Walter et al bioRxiv 2020.04.16.045419

Insert

Sb#45

Expression

Bacterial

Promoter

pBAD

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBbA2k-RFP

Plasmid

#35327

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Tet

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pET21a-TEX264-deltaLRR-His6

Plasmid

#220215

Purpose

Bacterial expression of TEX264, 2-25 residues (LRR) truncated, C-terminal His6 tag

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

His6

Expression

Bacterial

Mutation

c.4-75del / p.2-24del

Promoter

T7

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET-His6-SUMO-MproC145A

Plasmid

#172653

Purpose

Bacterial expression of SARS-CoV-2 Mpro C145A inactive mutant fused to SUMO

Depositor

Stephen Harrison

Article

MacDonald et al ACS Infect Dis. 2021 Sep 10;7(9):2591-25

Insert

SARS-CoV-2 Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

6xHis Tag and SUMO

Expression

Bacterial

Mutation

Catalytic Cysteine 145 mutated to Alanine

Promoter

T7

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pNIC-ZB

Plasmid

#26107

Purpose

SGC Empty backbone for bacterial expression

Depositor

Opher Gileadi

Article

Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

Type

Empty backbone

Tags

His6 - Z-basic - TEV

Expression

Bacterial

Available Since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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pQdC12a

Plasmid

#191356

Purpose

Clostridium CRISPRi plasmid with TetR-repressible dCas12a (LbCpf1) expression

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Inserts

PGusA2-TetO2/1-dCas12a (LbCpf1)
miniPthl-tetR

Expression

Bacterial

Promoter

PGus2A-TetO2/1 and miniPthl

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBP-P_tetR-PTet*

Plasmid

#204077

Purpose

Type 0 promoter part

Depositor

Mikhail Shapiro

Insert

tetR repressor and PTet* tetracycline-inducible promoter

Expression

Bacterial

Mutation

None

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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GST-Rab8a-TN

Plasmid

#86080

Purpose

A bacterial expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus GST.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

hRab8A (RAB8A Human)

Tags

GST

Expression

Bacterial

Mutation

Changed threonine 22 to aspargine

Promoter

tac

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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p0857 pBR322 SV40

Plasmid

#10877

Depositor

Peter Howley

Insert

SV40

Expression

Bacterial

Available Since

Oct. 26, 2005

Availability

Academic Institutions and Nonprofits only
pAC-BETAipi-ispA/dxs

Plasmid

#178302

Purpose

Produces beta-carotene in E. coli; pAC-BETAipi inserted with an operon containing ispA and dxs from E. coli.

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Inserts

ispA
dxs

Expression

Bacterial

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pSC27

Plasmid

#117478

Purpose

mobilizable, SRB-stable, kanamycin-resistant cloning vector

Depositor

Judy Wall

Article

Rousset et al Plasmid. 1998;39(2):114-22. doi: 10.1006

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCNA

Plasmid

#135191

Purpose

Arabinose inducible expression of lamba-Red recombinase proteins and I-CreI.

Depositor

Hongqing Fang

Article

Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

Insert

I-CreI lamba Red

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Promoter

araBAD

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pBRα

Plasmid

#53731

Purpose

Plpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.

Depositor

Ann Hochschild

Article

Dove et al Nature. 1997 Apr 10;386(6625):627-30.

Insert

αNTD

Expression

Bacterial

Promoter

lpp/lacUV5 (tandem promoter)

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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