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pET21-pelB-LaRIgG-2

Plasmid

#228996

Purpose

For bacterial expression of anti-rabbit IgG nanobody LaRIgG-2, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaRIgG-2

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaGIgG-12

Plasmid

#228999

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-12, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaGIgG-12

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-GST-3

Plasmid

#228985

Purpose

For bacterial expression of anti-GST nanobody GST-3, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-3

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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MZB134

Plasmid

#228485

Purpose

"Empty" N-terminal 6xHis-TEV-Halotag-HRV vector for easy DNA Assembly for pulldowns (Bacterial Expression)

Depositor

Richard Baker

Type

Empty backbone

Tags

6xHis-TEV-Halo-HRV

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pTBY1-T4uvsY

Plasmid

#228214

Purpose

Expression in BL21(DE3) of T4 UvsY-like recombination mediator

Depositor

Stephen Benkovic

Insert

uvsY (uvsY Escherichia phage T4)

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SnoopTag-AffiHER2-SpyTag

Plasmid

#216280

Purpose

Expresses an affibody to HER2 fused to SnoopTag and SpyTag, for bacterial expression

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

SnoopTag-AffiHER2-SpyTag

Use

Affinity Reagent/ Antibody

Tags

His6, SnoopTag, SpyTag, and Thrombin cleavage sit…

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD WT

Plasmid

#164022

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 UPA-CARD

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pROP-IP-FBFP

Plasmid

#122138

Purpose

FMN-based fluorescent protein Reporter plasmid for B-Rex contain imperfect operator sequence (anaerobic purpose)

Depositor

Srivatsan Raman

Article

Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

Insert

FBFP

Expression

Bacterial

Promoter

J23101

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-LgBiT-SpyCatcher

Plasmid

#223634

Purpose

Protein purification of SpyCatcher003 (S49C) with LgBiT and 6xHis tags from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Tags

His6, LgBiT

Expression

Bacterial

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAT593

Plasmid

#68909

Purpose

NanoLuc expressed constiutively from PBT1311 and rpiL* RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-2T_EV-D68_VP1

Plasmid

#202072

Purpose

Bacterial expression of VP1 protein from enterovirus D68 (EV-D68). Contains N-terminal GST-tag and C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

EV-D68 VP1 protein with Histag

Tags

6xHis-tag and GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-14-3-3 gamma GST

Plasmid

#13280

Depositor

Michael Yaffe

Article

Rittinger et al Mol Cell. 1999 Aug . 4(2):153-66.

Insert

14-3-3 gamma (Ywhag Rat)

Tags

GST

Expression

Bacterial

Available Since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only
pTetO-ISce1-TetO

Plasmid

#58506

Purpose

ISce1 restriction site is flanked by Tet operator repeats, 96 on each side

Depositor

Tom Misteli

Article

Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

Insert

Tet repeats flanking ISce1 restriction site

Expression

Bacterial

Promoter

no promoter

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pHH0103_GRB2-1/2

Plasmid

#91480

Purpose

Protein expression and purification of human SH3 domain construct GRB2-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

GRB2-1/2 (GRB2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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GLI K12

Plasmid

#16419

Depositor

Bert Vogelstein

Article

Kinzler et al Nature. 1988 Mar 24. 332(6162):371-4.

Insert

GLI (GLI1 Human)

Expression

Bacterial

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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human PNP non-tagged

Plasmid

#64199

Purpose

Expression of native human PNP (non-tagged)

Depositor

Vern Schramm

Article

Murkin et al Biochemistry. 2007 May 1;46(17):5038-49.

Insert

Purine Nucleoside Phosphorylase (PNP Human)

Tags

none

Expression

Bacterial

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pSLS.419

Plasmid

#187208

Purpose

Expresses wild-type retron Eco4 ncRNA, and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco4 ncRNA
Cas1 + Cas2

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7/Lac

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDDRFP-A1

Plasmid

#36289

Depositor

Robert Campbell

Article

Alford et al Chem Biol. 2012 Mar 23;19(3):353-60.

Insert

Dimerization dependent RFP A1

Tags

His tag

Expression

Bacterial

Available Since

April 26, 2012

Availability

Academic Institutions and Nonprofits only
pRG/III-hs-MBP

Plasmid

#121676

Purpose

Bacterial expression of MBP fusion proteins

Depositor

Reinhard Grisshammer, Richard Henderson

Article

Tucker et al Biochem J. 1996 Aug 1;317 ( Pt 3):891-9.

Insert

maltose binding protein

Expression

Bacterial

Promoter

lac promoter

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pET28b-irp3 WT

Plasmid

#87106

Purpose

expresses Irp3 WT, the thiazolinyl imine reductase from Yersinia enterocolitica

Depositor

Audrey Lamb

Article

Meneely et al Biochemistry. 2012 Nov 6;51(44):9002-13.

Insert

irp3 WT

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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