30,808 results

1. pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-I48V_AnaCNJKFGW_Bba-B0015

   Plasmid

   #202023

   Purpose

   Expresses Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant) in E. coli

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al bioRxiv 2024.03.30.587094

   Insert

   Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant)
   

   Expression

   Bacterial

   Mutation

   GvpA T6A-I48V

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTns(Ptr)

   Plasmid

   #175576

   Purpose

   Expression of CRISPR-associated transposases

   Depositor

   Sheng Yang

   Article

   Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

   Insert

   PtrTnsABC
   

   Expression

   Bacterial

   Mutation

   NO

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJJS007

   Plasmid

   #188330

   Purpose

   PCR template for repair templates to tag a gene with a wrmScarlet and mIAA7 AID degron using homology-directed repair for C. elegans.

   Depositor

   Mike Boxem

   Article

   Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

   Insert

   mIAA7::wrmScarlet::Linker
   

   Expression

   Bacterial

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCPP5910

   Plasmid

   #128717

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

   Insert

   shcO-hopO1-1
   

   Expression

   Bacterial

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCPP5058

   Plasmid

   #128710

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

   Insert

   shcF-hopF2
   

   Expression

   Bacterial

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. human MTAP

   Plasmid

   #64077

   Purpose

   Expression of human 5'-methylthioadenosine phosphorylase

   Depositor

   Vern Schramm

   Article

   Guan et al Biochemistry. 2011 Nov 29;50(47):10408-1

   Insert

   5'-methylthioadenosine phosphorylase (MTAP Synthetic, Human)
   

   Tags

   Histidine tag and TEV

   Expression

   Bacterial

   Available Since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFLxR5

   Plasmid

   #149482

   Purpose

   Broad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   LuxR-mRFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PLuxB

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMM585

   Plasmid

   #68884

   Purpose

   IPTG-inducible NanoLuc expression, pNBU2 backbone, AmpR

   Depositor

   Timothy Lu, Christopher Voigt

   Article

   Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

   Inserts

   NanoLuc
   LacI
   

   Expression

   Bacterial

   Available Since

   Oct. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBK086

   Plasmid

   #183145

   Purpose

   pET-6xHis-MBP-TEV-MapTIR-APAZ/MapAgo - Heterolgous MapSPARTA expression

   Depositor

   Daan Swarts

   Article

   Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

   Insert

   MapSPARTA operon (6xHis-MBP-MapTIR-APAZ and MapAgo)
   

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJMP8045

    Plasmid

    #220900

    Purpose

    Tn7 transposase (tnsABCD) expression plasmid for Rhodobacter sphaeroides

    Depositor

    Jason Peters

    Article

    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

    Insert

    tnsABCD
    

    Expression

    Bacterial

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. His-SUMO-hDDI2-D252N_pET11a

    Plasmid

    #169122

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hDDI (DDI2 Human)
    

    Expression

    Bacterial

    Mutation

    D252N

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXW117As(RS-E11-RinA)2

    Plasmid

    #123146

    Purpose

    Arsenic sensor with three-layered amplifier cascade. Need to work with plasmid pXWPrinA-gfp2. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J117, ParsR, PhrpLE, Pecf11

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXWPrinA-gfp2

    Plasmid

    #123147

    Purpose

    pSB1K3 carrying PrinA-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    PrinA-30gfp-t
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PrinA

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOPINN-GFP

    Plasmid

    #53541

    Purpose

    pTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus system

    Depositor

    Ray Owens

    Insert

    GFP
    

    Tags

    N-His-GFP

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOPINB-hRNF216(457-784)

    Plasmid

    #171922

    Purpose

    Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic helix-RBR-helix construct suitable for activity assays.

    Depositor

    Bernhard Lechtenberg

    Article

    Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

    Insert

    E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
    

    Tags

    3C protease cleavage site and 6x-His tag

    Expression

    Bacterial

    Mutation

    codon optimised for expression in E. coli

    Promoter

    T7

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTE105

    Plasmid

    #59400

    Purpose

    MethyloBrick expression vector, tuf promoter; TcR

    Depositor

    Tobias Erb

    Article

    Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Ptuf

    Available Since

    Dec. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTriEx-RhoA-Q63L_mScarlet-i_SGFP2

    Plasmid

    #85072

    Purpose

    Green/red FRET biosensor for RhoA in the GTP locked constitutively active state

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    RhoA Biosensor constitutively active (Q63L) (RHOA Human)
    

    Tags

    6xHis and mScarlet-i and SGFP2

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Q63L

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET16b-Rnl2(1-249)K227Q

    Plasmid

    #14072

    Depositor

    Thomas Tuschl

    Insert

    Rnl2(1-249)
    

    Tags

    His (10x)

    Expression

    Bacterial

    Mutation

    changed Lys227 to Glutamine Gly112 is actually C…

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

19. zbFGF

    Plasmid

    #12309

    Depositor

    James Thomson

    Article

    Ludwig et al Nat Methods. 2006 Aug . 3(8):637-646.

    Insert

    Fgf2 (fgf2 Zebrafish)
    

    Expression

    Bacterial

    Mutation

    Ala 11 mutated to Pro, Ser 55 mutated to Asn

    Available Since

    Sept. 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSCW02

    Plasmid

    #72301

    Purpose

    Starting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Type

    Empty backbone

    Use

    Derivative of puc19

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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