30,320 results

1. SOCS4B-c002

   Plasmid

   #53669

   Purpose

   bacterial expression plasmid

   Depositor

   Nicola Burgess-Brown

   Insert

   SOCS4B
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Bacterial expression for structure determination;…

   Promoter

   Available since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pU6T7

   Plasmid

   #71462

   Purpose

   Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (2 Gs at initiation)

   Depositor

   Willie Mark

   Article

   Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   human U6/phage T7

   Available since

   Feb. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTCF200 Mouse beta-2 microglobulin

   Plasmid

   #180462

   Purpose

   E. coli expression of class I MHC light chain

   Depositor

   John Altman

   Insert

   Mouse beta-2 microglobulin
   

   Use

   Tags

   BSP41

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pZac2.1 gfaABC1D-Glt1-eGFP

   Plasmid

   #176862

   Purpose

   Expresses Glt-1 fused with GFP at the C-terminus in astrocytes

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   Glt1-eGFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   gfaABC1D

   Available since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pENTR R4 ATCC LacZ GCTT

   Plasmid

   #173668

   Purpose

   Destination vector for Golden Gate Cloning assemblies, blue-white screening

   Depositor

   Eric Marois

   Article

   Green et al Elife. 2023 Dec 5;12:e93142. doi: 10.755

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX6P1-optimizedNedd4.2d

   Plasmid

   #165103

   Purpose

   bacterial expression of a GST fusion of Human Nedd4.2d

   Depositor

   Arthur Haas

   Article

   Todaro et al J Biol Chem. 2017 Nov 24;292(47):19521-1

   Insert

   Nedd4.2d (NEDD4L Human)
   

   Use

   Tags

   GST from plasmid.

   Expression

   Bacterial

   Mutation

   codon optimized for E. coli expression

   Promoter

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAT9658-sgRNA-mCherry

   Plasmid

   #162987

   Purpose

   sgRNA cloning plasmid

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   exchangeable cassette
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p2CT-His-MBP-Hhe_Cas13a_WT

   Plasmid

   #91871

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   HheCas13a (HheC2c2) WT
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p_SNIPR_sensor_GFP

   Plasmid

   #139463

   Purpose

   T7 RNAP-driven expression of SNIPR RNA with a GFP-ASV reporter

   Depositor

   Alexander Green

   Article

   Hong et al Cell. 2020 Feb 25. pii: S0092-8674(20)30

   Insert

   SNIPR_GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAJM.715

    Plasmid

    #108516

    Purpose

    PTac-YFP Reporter. IPTG sensor for use in Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    PTac-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMO746

    Plasmid

    #117480

    Purpose

    upp in an artificial operon with npt from pMO9071 and AmpR-pUC ori from pCR4/TOPO, Pnpt-npt-upp;, KanR

    Depositor

    Judy Wall

    Article

    Parks et al Science. 2013 Mar 15;339(6125):1332-5. d

    Insert

    uracil phosphoribosyltransferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKD227

    Plasmid

    #90954

    Purpose

    Expresses S. baltica TtrS under the Ptac promoter

    Depositor

    Jeffrey Tabor

    Article

    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

    Insert

    Tetrathionate sensor kinase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHH0103_DNASE1_Exo-endo-phos

    Plasmid

    #110004

    Purpose

    Protein expression and purification of DNASE1_Exo-endo-phos

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    DNASE1_Exo-endo-phos (DNASE1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET Biotin His6 mOrange LIC cloning vector (H6-mOrange)

    Plasmid

    #29723

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, AviTag (biotin), TEV cleavage site, and mO…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAT202

    Plasmid

    #134879

    Purpose

    Bacterial expression

    Depositor

    Thomas Howard

    Article

    Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    deletion of Y208

    Promoter

    Available since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDx_mRuby3-SYFP2

    Plasmid

    #189758

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mRuby3-SYFP2 fusion protein for FRET analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mRuby3-SYFP2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV & Rha

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Salmonella MTAN

    Plasmid

    #64041

    Purpose

    Expression of S. enterica MTAN in E. coli

    Depositor

    Vern Schramm

    Article

    Hemeon et al Anal Chem. 2011 Jun 15;83(12):4996-5004.

    Insert

    Methylthioadenosine nucleosidase
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

18. GLI K12

    Plasmid

    #16419

    Depositor

    Bert Vogelstein

    Article

    Kinzler et al Nature. 1988 Mar 24. 332(6162):371-4.

    Insert

    GLI (GLI1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a(+)-SpdCas9-LBiT_Strep-Tag-II

    Plasmid

    #198751

    Purpose

    Expresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferase

    Depositor

    Maarten Merkx

    Article

    van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

    Insert

    SpdCas9 - LargeBiT
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    D10A, H840A (nuclease inactivating mutations in &…

    Promoter

    T7

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDF RBP-FL P53

    Plasmid

    #174042

    Purpose

    Express Full length P53 in E.coli with cleavable expression/purification tag (ribose binding protein)

    Depositor

    Stewart Loh

    Article

    Blanden et al Elife. 2020 Dec 2;9. pii: 61487. doi: 10

    Insert

    P53 gene (TP53 Human)
    

    Use

    Tags

    Thermoanaerobacter Tencongenesis ribose binding p…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge