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34,235 results

Showing: 6781 - 6800 of 34235 results

  5. pSL8215 (dCas12f–σE (sigmaE) system, entry vector)

    Plasmid
    #254272
    Purpose
    Encodes E. coli codon-optimized dCas12f and σE (sigmaE) from Flagellimonas taeanensis driven by constitutive promoters, with BsaI-containing restriction sites for guide RNA cloning
    Depositor
    Samuel H. Sternberg
    Publication
    Hoffmann et al Nature. 2026 Mar 4. doi: 10.1038/s41586-
    Inserts
    dCas12f
    sigma E
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    J23105
    Available since
    April 6, 2026
    Availability
    Academic Institutions and Nonprofits only
    254272-plasmid-map-sequence-id-505316

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  8. pAIDA_TEV_fap2EC

    Plasmid
    #246866
    Purpose
    Expression of Fusobacterium nucleatum ATCC23726 Fap2 extracellular domain (42-3271) with histag and TEV site in fusion with E. coli autotransporter AIDA
    Depositor
    Daniel Roderer
    Publication
    Schopf et al Nat Commun. 2025 Aug 29;16(1):8104. doi:
    Insert
    F. nucleatum ATCC23726 Fap2 (extracellular domain, res. 42–3271)
    Tags
    8x histidine, AIDA autotransporter, AIDA signal s…
    Expression
    Bacterial
    Available since
    Nov. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    246866-plasmid-map-sequence-id-493107

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  11. pEF1-Pls3-sfGFP

    Plasmid
    #205750
    Purpose
    Expresses sfGFP under IPTG inducible promoter Pls3 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Medium/High strength.
    Depositor
    Jerome Bonnet
    Publication
    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95
    Type
    Empty backbone
    Use
    Shuttle vector gram+ gram-
    Expression
    Bacterial
    Promoter
    Pls3 (Phyperspank mutant, IPTG inducible)
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    205750-plasmid-map-sequence-id-478438

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  13. pMS01

    Plasmid
    #230064
    Purpose
    For allelic exchange into Bacteroides fragilis (pLGB13-based); ermC, aTC-inducible counterselection with ssbfe1; R6K ori, RP4 oriT
    Depositor
    Sean Crosson
    Publication
    Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(
    Type
    Empty backbone
    Use
    Synthetic Biology; Allelic exchange
    Expression
    Bacterial
    Promoter
    None
    Available since
    June 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    230064-plasmid-map-sequence-id-475946

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  14. cBEST4

    Plasmid
    #234660
    Purpose
    Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion
    Depositor
    Mingzi M. Zhang
    Publication
    Lin et al Appl Environ Microbiol. 2025 Mar 10:e019
    Inserts
    spCas9 cytosine base editor
    Golden Gate compatible sgRNA insertion cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P3 and tcp830
    Available since
    May 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    234660-plasmid-map-sequence-id-472864

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  17. PKA-N-HiBiT

    Plasmid
    #211377
    Purpose
    Bacterially expressed HiBiT-PKA for Ni-NTA purification
    Depositor
    James Inglese
    Publication
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Human protein kinase cAMP-activated catalytic subunit alpha (PRKACA) transcript variant 1, with N-terminal HiBiT (PRKACA Human)
    Tags
    10xHis-tag with thrombin site and HiBiT Tag
    Expression
    Bacterial
    Promoter
    T7 Promotor
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    211377-plasmid-map-sequence-id-417163

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Showing: 6781 - 6800 of 34235 results