32,417 results

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pCDB179-BhrPETase

Plasmid

#218692

Purpose

Encodes codon optimized BhrPETase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

BhrPETase

Tags

10xHis

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCBFD2Bad

Plasmid

#53574

Purpose

Produces an active CBFD (carotenoid β-ring 4-dehydrogenase) enzyme in E. coli when expression is induced with arabinose

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

Insert

cbfd2

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pEC3006 (pSpy1C-PZn_ffluc)

Plasmid

#218515

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenes

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Expression

Bacterial

Mutation

deletion of Plac_mrfp cassette

Promoter

PZn (PczcD)

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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5 pCOLI_A_STREP

Plasmid

#160147

Purpose

N-term 2xStrepII 3C cleavable Amp resistant bacterial expression vector

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Tags

2xStrepII

Expression

Bacterial

Promoter

T7

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-FOLD6

Plasmid

#186547

Purpose

FOLD6 "hyperfolder" green fluorescent protein: bacterial expression plasmid

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

FOLD6

Tags

6xHis

Expression

Bacterial

Mutation

mGreenLantern-C48S/V68L/C70V/K101E/T105Y/S147P/K1…

Promoter

araB

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pGMC00018 (aka pRC0082)

Plasmid

#195320

Purpose

Minimal plasmid backbone with multiple cloning site for HDR donor DNA construction

Depositor

Raj Chari

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial and Mammalian

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pQIq_MRGS_HIS8_(27F3)_FLAG

Plasmid

#199615

Purpose

Encodes DARPin-FLAG 27F3 for bacterial expression and column purification

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

DARPin-FLAG 27F3

Tags

8xHis and FLAG

Expression

Bacterial

Promoter

T5

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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MBP-MEK1

Plasmid

#68300

Purpose

N-terminal MBP tagged human MEK1 gene for bacterial expression

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

MBP-MEK1 (MAP2K1 Synthetic, Human)

Tags

His and MBP

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pJKR-H-mphR

Plasmid

#62559

Purpose

Macrolide biosensor. High copy plasmid that expresses GFP proportional to macrolide concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pMyBADC

Plasmid

#84688

Purpose

Protein expression in mycobacteria

Depositor

Matthias Wilmanns

Article

Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

Type

Empty backbone

Use

Bacterial expression vector

Tags

His6

Expression

Bacterial

Promoter

Arabinose

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-SUMO1

Plasmid

#185637

Purpose

expression of GST-tagged SUMO1 in bacterial cells

Depositor

Irina Bezsonova

Article

Bregnard et al Biophys Chem. 2022 Aug;287:106827. doi:

Insert

small ubiquitin-like modifier 1 (SUMO1 Human)

Tags

GST

Expression

Bacterial

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBT-2

Plasmid

#22833

Depositor

Ryan Gill

Article

Lynch et al Biotechnol Bioeng. 2006 May 5. 94(1):151

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

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pRF-PhlF

Plasmid

#49367

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

PhlF repressor

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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ptrc99a-GPPS-CSstr-ispA*[JBEI-15060]

Plasmid

#100962

Purpose

Contains genes encoding GPP synthase, cineole synthase and IspA(S81F)

Depositor

Taek Soon Lee

Insert

GPPS-CSstr-ispA*

Expression

Bacterial

Promoter

trc

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pAH69

Plasmid

#173782

Purpose

The pAH69 plasmid [Haldimann and Wanner 2001] is the HK022 helper plasmid necessary for genomic integration of the pIT5_KH plasmid.

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Insert

HK022 Integrase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAraGFPCDF

Plasmid

#47516

Purpose

Contains PBAD::GFP reporter, used for heterodimer competition assay

Depositor

Bryan Berger

Article

Su et al J Mol Biol. 2013 Jul 19. pii: S0022-2836

Insert

GFP

Expression

Bacterial

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pBS Wnt-2HA

Plasmid

#17368

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-2HA (WNT2 Human)

Tags

HA

Expression

Bacterial

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
Nano-lantern/pRSETB

Plasmid

#51969

Purpose

bacterial expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Tags

6xHis

Expression

Bacterial

Mutation

S257G in Rluc8

Available Since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

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pEF1-Pls4-sfGFP

Plasmid

#205751

Purpose

Expresses sfGFP under IPTG inducible promoter Pls4 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. High strength.

Depositor

Jerome Bonnet

Article

Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95

Type

Empty backbone

Use

Shuttle vector gram+ gram-

Expression

Bacterial

Promoter

Pls4 (Phyperspank mutant, IPTG inducible)

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pKEK3230

Plasmid

#234102

Purpose

CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

Depositor

Karl Klose

Article

Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

Insert

FuGFP

Expression

Bacterial

Promoter

pJ23100-BsaI

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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