31,813 results
-
Plasmid#189850PurposeCre-inducible activation of knt for kanamycin resistance.DepositorInsertP*-loxP-TT-loxP-R-knt
UseCre/Lox and Synthetic BiologyExpressionBacterialPromoterP* (modified T7 phage gene 10)Available SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
BRD9
Plasmid#39012PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 IQGAP1 WW domain
Plasmid#104279PurposeBacterial expression of WW domain from IQGAP1DepositorInsertIQGAP1 WW domain (IQGAP1 Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBSII(KSplus)-HsBGG-WT_E
Plasmid#146187PurposeBacterial Expression of HsBGG-WTDepositorInsertHsBGG-WT
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
RCP83
Plasmid#163466PurposeBackbone plasmid for insertion of the Saccharomyces cerevisiae promoter library and yeGFPDepositorInsertsPromoter fragment (Sharon2012_1922)
His3 minimal promoter
yeGFP
ExpressionBacterial and YeastAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT25
Plasmid#89664Purposeexpression of doubleTAL staple protein dT25 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT25
TagsHis6ExpressionBacterialPromoterT7Available SinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT19
Plasmid#89661Purposeexpression of doubleTAL staple protein dT19 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT19
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJKR-H-acuR
Plasmid#62567PurposeAcrylate biosensor. High copy plasmid that expresses GFP proportional to acrylate concentration.DepositorInsertGFP
ExpressionBacterialAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pM9-BetaGal-8C
Plasmid#172489PurposePlasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequenceDepositorInsertM9-BetaGal-8C
Tags6xHis and M9 nuclear localization sequence (from …ExpressionBacterialMutationBetaGal has two cysteine mutations (C77A, C1022S)…PromoterT7Available SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
RAB1A
Plasmid#25521PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 24, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pM1s3AsR_TS-C7
Plasmid#137945PurposeE. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive RFP expression and strong temperature sensitive GFP expressionDepositorInsertmRFP1
UseSynthetic Biology; For use with e. coli nissle in…ExpressionBacterialPromoterJ23101Available SinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX Socs1-SH2
Plasmid#46504DepositorInsertSocs1 (SOCS1 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1-166PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-TFIIE
Plasmid#171083PurposeCo-expresses human TFIIE. The resulting plasmid was used to generate a single expression construct encoding 2 subunits, with His-tag on TF2E1.Originally from MN Gonzalez, was submitted with permissionDepositorTagsHisExpressionBacterialPromoterT7 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHis-LMW_FGF2
Plasmid#157657PurposeExpresses FGF2 in E.coli cellDepositorInsertFibroblast growth factor 2 (FGF2 Human)
TagsHis tagExpressionBacterialMutationchanged Cystein 211,229 to serine, deleted amino …Available SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+bromo5
Plasmid#157803PurposeExpression of synthetic protein composed of 5 repeats of BRD4 bromodomain #1DepositorInsertbromo5
TagsHis6 and MBPExpressionBacterialPromoterpTACAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
SHMT2
Plasmid#25479PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTrc99a-NoSS-TolC
Plasmid#155179PurposeExpresses signal sequence-less TolC as inclusion bodiesDepositorInsertTolC (tolC )
ExpressionBacterialMutationdeleted residues 1-20, residue 21 mutated to Met …PromotertrcAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD132
Plasmid#119885PurposeIntegration vector into the ROCI-3 site of Rhodococcus opacus PD630 containing constitutive EYFP and hygromycin B resistance markerDepositorInsertBbaJ23104_EYFP
ExpressionBacterialAvailable SinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSC226
Plasmid#216375PurposexhNup358-Nb2t (+3Cys) Nanobody productionDepositorInsertxhNup358-Nb2t (+3Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-53BP1LCD
Plasmid#204407PurposeE. coli expression of the 53BP1 low-complexity domain (1208-1977) with an N-terminal mCherry tagDepositorAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only