32,934 results
-
Plasmid#171925PurposeBacterial expression of nanobody VHH_C5, which binds to receptor binding domain of the spike protein of SARS-CoV-2DepositorInsertVHH_C5
TagsHis6ExpressionBacterialAvailable SinceSept. 23, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAT9679-sgRNA-BFP
Plasmid#162988PurposesgRNA cloning plasmidDepositorInsertexchangeable cassette
UseCRISPRExpressionBacterial and MammalianPromoterU6Available SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SUMO-AsCas12f
Plasmid#171612PurposeAsCas12f1 heterologous expression plasmidDepositorInsertAsCas12f1
ExpressionBacterialAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB207
Plasmid#164122PurposeJ23101 driving PP7 repeatsDepositorInsertYFP-PP7
ExpressionBacterialPromoterJ23101Available SinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBad-UGAcS
Plasmid#172137Purposebacteria expression plasmid for a UDP-GlcNAc sensorDepositorInsertUGAcS
ExpressionBacterialMutationH605Q- please see depositor commentsPromoteraraBADAvailable SinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-41
Plasmid#172757PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-41
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBad-UXPS
Plasmid#172138Purposebacteria expression plasmid for a UDP/UTP sensorDepositorInsertUXPS
ExpressionBacterialPromoteraraBADAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HaloTag7SS-DogTag
Plasmid#171775PurposeExpresses in bacterial cytoplasm HaloTag7 with DogTag inserted between D139 and E140, as well as C61S and C261S in HaloTag7 to block disulfide bond formationDepositorInsertHaloTag7SS-DogTag
TagsHis6ExpressionBacterialMutationDogTag inserted in HaloTag7 between residues D139…PromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET32a-Trx-His6-TEV_SacB
Plasmid#164588PurposeBacterial expression plasmid with sacB gene. Suitable for LIC cloning of a gene insert replacing the sacB.DepositorInsertTEV-SacB
TagsTrx-His6 followed by TEV siteExpressionBacterialPromoterT7Available SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTS_TXS
Plasmid#163839PurposeiPTG inducible expression of taxadiene synthase and geranylgeranyl diphosphate synthaseDepositorInserttaxadiene synthase (61-862)
ExpressionBacterialPromoterTrcAvailable SinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCas9-beta
Plasmid#169240PurposeCas9 and beta expression plasmid for performing gene knockouts via oligo recombineeringDepositorInsertscas9
beta
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBJ3
Plasmid#167132PurposeA kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoterDepositorInsertluxCDABE
UseLuciferaseExpressionBacterialPromoterFrr promoterAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGV3341
Plasmid#170279PurposeExpression of mutant HIV-2 RT p66 reverse transcriptaseDepositorInsertHIV-2 RT p66 (mutant)
ExpressionBacterialPromoterT7-lacAvailable SinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS29: pDonor(PmcCAST)
Plasmid#168162PurposePmcCAST donor harboring non-functional cargo.DepositorInsertsPmcCAST right end (RE)
PmcCAST left end (LE)
ExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNIC-AncCAR_A
Plasmid#168286PurposeExpresses AncCAR_A in E. coli from a T7 promoterDepositorInsertAncCAR_A
UseSynthetic BiologyTags6*HisExpressionBacterialPromoterT7Available SinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPM2213
Plasmid#167553PurposeEncodes rfb region of Shigella flexneri serotype 2a, which expresses Shigella O antigen in E. coli K-12DepositorInsertrfb region of Shigella flexneri serotype 2a
ExpressionBacterialAvailable SinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
APneg-SNAP25a
Plasmid#168011Purposenegative selection PACE Accessory PlasmidDepositorInsertSNAP25-T7PAP
ExpressionBacterialAvailable SinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2
Plasmid#166972PurposeExpresses FBF-2 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
TagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575PromoterT7Available SinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
p28BIOHTEV-LIC
Plasmid#167852PurposeBacterial expression vector with N-terminal AviTag and C-terminal 6X-his tag for protein expression and purificationDepositorTypeEmpty backboneTags6xHis and AviTagExpressionBacterialAvailable SinceApril 22, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-TEV-TBP6.9F34MF37MF77M
Plasmid#168268PurposeExpresses human TBP6.9 with the F34M/F37M/F77M triple mutantDepositorInsertTBP6.9
Tags6xHis tag N-terminus, TEV protease cleavage siteExpressionBacterialMutationchanged F34M, F37M and F77MPromoterT7 promotorAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only