31,824 results
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Plasmid#182291Purposeexpresses c-di-GMP biosensor in E. coliDepositorInsertmVenusNB-MrkH
TagsmVenusNB internal fusion to MrkHExpressionBacterialPromoterproCAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-NEMO
Plasmid#11965DepositorAvailable SinceJuly 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
PDCD1-DEM (P1#5)
Plasmid#227738PurposeDesigner Epigenome Modifier (DEM) designed to target the PDCD1-promoterDepositorInsertDesigner epigenome modifier targeting PDCD1-promoter
UseTALENTagsDNMT3A, DNMT3L, KRABExpressionBacterial and MammalianPromoterCMVAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
LAG3-DEM (L3#1)
Plasmid#227739PurposeDesigner Epigenome Modifier (DEM) designed to target the LAG3-promoterDepositorInsertDesigner epigenome modifier targeting LAG3-promoter (Homo sapiens)
UseTALENTagsDNMT3A, DNMT3L, KRABExpressionBacterial and MammalianPromoterCMVAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
GreenT-EC - pRSET
Plasmid#193947PurposeBacterial expression vector for a low-affinity calcium indicatorDepositorInsertGreenT-EC
TagsHis-TagExpressionBacterialAvailable SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
FnR8-10
Plasmid#182569PurposeHuman fibronectin fragment, type-III repeats 8-10, R1379A mutantDepositorInsertHuman fibronectin fragment, type-III repeats 8-10
TagsHis and TEV cleavage site after His tagExpressionBacterialMutationR1379AAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE075
Plasmid#176832PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert714
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCJ191
Plasmid#162668PurposepET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker
TagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET22b-csgB
Plasmid#89582PurposeExpress csgB curli protein with GS linker and HistaqDepositorAvailable SinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
t1-435
Plasmid#166578PurposeFor expression of human talin head (residues 1-435) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.DepositorInsertTln1 Head (1-435) (TLN1 Human)
TagsHis6-tag, Xpress-epitope (DLYDDDDK) and enterokin…ExpressionBacterialMutationresidues 1-435 onlyAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-T7-NlaCas3-NLS-6xHis
Plasmid#180214PurposeExpressing Neisseria lactamica Type I-C Cas3 with NLS and His tag on the C terminus for protein purification in E.ColiDepositorInsertNlaCas3c
Tags6xHis and NLSExpressionBacterialPromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
TOPO GFP
Plasmid#68488PurposeTOPO construct expressing GFP for generation of GMAP-compatible geneDepositorInsertGreen Fluorescent Protein
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRAU168
Plasmid#86834Purposebacterial expression of anti-CRISPR protein AcrIIA1DepositorInsertacrIIA1
ExpressionBacterialPromoteraraBADAvailable SinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET-B CFP
Plasmid#108858PurposeEncodes for the fluorescent protein CFPDepositorInsertCFP
ExpressionBacterialAvailable SinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
6CC8 (MBD3)
Plasmid#119959PurposeBacterial expression for structure determinationDepositorInsertMBD3 (MBD3 Human)
TagsGSTtag, 6xHis and ThrombinExpressionBacterialMutationMBD domain (contains residues 1-71)PromoterT7Available SinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMRE-Tn7-130
Plasmid#118548PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertTagBFP2
UseMinitn7 delivery vectorExpressionBacterialAvailable SinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC575
Plasmid#127976PurposeAMA1 plasmid with ble selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertble (bleomycin resistance marker)
UseFungal expressionExpressionBacterialPromoterAspergillus nidulans trpC promoterAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHB1
Plasmid#212153PurposeFor PHB production in E. coli (PT7-pMB1).DepositorInsertpoly(3-hydroxyalkanoate) polymerase subunit PhaC, PHA-specific beta-ketothiolase, acetoacetyl-CoA reductase
ExpressionBacterialPromoterPT7Available SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
csr-1_son-1_mNeonGreen
Plasmid#191743PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
TagsNCU04502 promoter and mNeonGreenExpressionBacterialAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
p11d-tRPA-32Ala9
Plasmid#102616PurposeExpression of human RPA with an alanine substituted phosphorylation domain (with the following mutations in RPA32 :S8,11-13,23,29,33,39A,T21A).DepositorExpressionBacterialMutationwith an alanine substituted phosphorylation domainPromoterT7Available SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only