30,327 results

1. HOXB7 (human) FLAG pSP65

   Plasmid

   #8537

   Depositor

   Corey Largman

   Article

   Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

   Insert

   HOXB7 (HOXB7 Human)
   

   Use

   In vitro transcription/translation

   Tags

   FLAG

   Expression

   Bacterial

   Mutation

   starts at AA#1 preceeded by FLAG and Ser-Arg-Ile…

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pET22-His-TEV-DOT1L

   Plasmid

   #89610

   Purpose

   Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L residues 1-416

   Depositor

   Leemor Joshua-Tor

   Article

   Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

   Insert

   Dot1L (DOT1L Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCD564

   Plasmid

   #153026

   Purpose

   dxCas9(3.7), MCP-SoxS(R93A/S101A)

   Depositor

   Jesse Zalatan

   Article

   Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

   Insert

   dxCas9(3.7), MCP-SoxS(R93A/S101A)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   SoxS has R93A and S101A mutations

   Promoter

   Available since

   Aug. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKRR5

   Plasmid

   #149468

   Purpose

   Broad-Host range expression vector, RK2 and pUC origin, RhaR-RhaS activator, PrhaBAD promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   RhaS-RhaR-mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PrhaBAD

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-mCherry-2xEcMTS

   Plasmid

   #133624

   Purpose

   Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.

   Depositor

   Petra Schwille

   Article

   Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

   Insert

   mCherry-2xEcMTS
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAD/HisB-iRFP682

   Plasmid

   #45454

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

   Insert

   iRFP682
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   T2A, S13L, A92T, V104T, V114I, E161K, E180V, Y193…

   Promoter

   araBAD

   Available since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSFDuet-1 ElonginB, ElonginC

   Plasmid

   #230999

   Purpose

   Dual expression vector containing mouse ElonginB and ElonginC for E.coli protein purification

   Depositor

   Michael Rape

   Article

   Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

   Inserts

   Elongin B (Elob Mouse)
   Elongin C (Eloc Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. 4QQD

   Plasmid

   #61947

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   UHRF1:SDC130-H02:C39674 (UHRF1 Human)
   

   Use

   Tags

   6xHis and MHHHHHHSSGRENLYFQG

   Expression

   Bacterial

   Mutation

   See comments

   Promoter

   T7

   Available since

   March 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pGI3EM20C

   Plasmid

   #135487

   Purpose

   Agrobacterium T-DNA with divergent Spun H2A/Bpr driving H2B-tdTomato and hph

   Depositor

   Nicolas Buchler

   Article

   Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

   Insert

   hph SpunH2A/Bpr H2B-tdTomato
   

   Use

   Tags

   tdTomato

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   SpunH2A/Bpr (divergent)

   Available since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPBC-LG3-tdT

    Plasmid

    #62810

    Purpose

    pPBC-LG3-tdT expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP3 and tdTomato under the control of the CAG promoter.

    Depositor

    Karen Wilcox

    Article

    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

    Insert

    ITR-CAG-Lck-GCaMP3-IRES-tdTomato-ITR
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOPINVHH_his

    Plasmid

    #210405

    Purpose

    For expression of VHH with C-terminal his tag

    Depositor

    Ray Owens

    Article

    Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

    Type

    Empty backbone

    Use

    Tags

    his6

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. p404GAL1

    Plasmid

    #17417

    Depositor

    Fred Cross, Nicolas Buchler

    Insert

    GAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    GAL1 promoter is between SacI and XbaI restrictio…

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28Teb1

    Plasmid

    #28173

    Depositor

    Kathleen Collins

    Article

    Min et al Mol Cell. 2009 Nov 25. 36(4):609-19.

    Insert

    Teb1
    

    Use

    Tags

    6xhistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pLITMUS-rpoN-cI-J23106-geneIII

    Plasmid

    #80843

    Purpose

    Phagemid (encodes M13 gene III and lambda cIopt).

    Depositor

    Mark Isalan

    Article

    Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

    Inserts

    M13 gene III
    Lambda cI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23106 and rpoN

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. M58 pPET28b-TEV-full-length zebrafish beta catenin

    Plasmid

    #17199

    Depositor

    Randall Moon

    Insert

    beta catenin (ctnnb1 Zebrafish)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

16. H2BK120C

    Plasmid

    #75332

    Purpose

    Bacteria expression of H2BK120C

    Depositor

    Cynthia Wolberger

    Article

    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

    Insert

    H2BK120C (h2bc11.S Frog)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    truncated; K120C

    Promoter

    T7

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQTEV-CIP29

    Plasmid

    #34822

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    cytokine induced protein 29 kDa (SARNP Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC009 LshCas13a locus with targeting spacer

    Plasmid

    #91900

    Purpose

    LshCas13a locus into pACYC184 with targeting spacer

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LshCas13a locus with targeting spacer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pTNSQCas

    Plasmid

    #219434

    Purpose

    CRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegens

    Depositor

    Sheng Yang

    Article

    Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

    Insert

    TnsA, TnsB, TnsC, TniQ, Cas8, Cas7, Cas6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDonor-LoxP

    Plasmid

    #219436

    Purpose

    CRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegens

    Depositor

    Sheng Yang

    Article

    Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

    Insert

    two transposon ends, LoxP-terminator-LoxP gene cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge