30,810 results

1. pALS099 (pGEX-2TP-cTevHis-DEST)

   Plasmid

   #139787

   Purpose

   Gateway destination vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis

   Depositor

   Jeannie Lee, Arneet Saltzman

   Article

   Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi:

   Type

   Empty backbone

   Tags

   6xHis (cleavable by TEV protease) and GST (cleava…

   Expression

   Bacterial

   Available Since

   April 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMVV207

   Plasmid

   #215343

   Purpose

   gRNA expression for S. stutzeri nifL deletion on an RSF1010 backbone

   Depositor

   Brian Pfleger

   Article

   Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

   Insert

   S. stutzeri nifL gRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMVV205

   Plasmid

   #215342

   Purpose

   Recombineering plasmid with constitutively expressed cas9 and the araBAD promoter expressing αβγ on a BBR1 backbone

   Depositor

   Brian Pfleger

   Article

   Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23105

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHH0103_BCAR1-1/1

   Plasmid

   #91418

   Purpose

   Protein expression and purification of human SH3 domain construct BCAR1-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   BCAR1-1/1 (BCAR1 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGTvL1-SGC

   Plasmid

   #39188

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Nicola Burgess-Brown

   Type

   Empty backbone

   Tags

   GST and TEV cleavage site

   Expression

   Bacterial

   Promoter

   Tac (lactose/IPTG inducible)

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

6. ATG-1929

   Plasmid

   #108712

   Purpose

   CBR2opt in pF4Ag expression vector with T7 and CMV promoter

   Depositor

   Keith Wood

   Article

   Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10

   Insert

   CBR2opt
   

   Expression

   Bacterial and Mammalian

   Mutation

   Promega used directed evolution to create CBR (Cl…

   Promoter

   T7, CMV

   Available Since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGBW-m4046920

   Plasmid

   #145717

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp3

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pAAP003l2

   Plasmid

   #209748

   Purpose

   RepA(N99D) SC101 T0-BS7 araC pBAD SD8 NpuC-C[AGGA]LFVY-NpuN-SsrA ProK 5 tRNA operon tetA-orfL bla

   Depositor

   Ahmed Badran

   Article

   Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

   Insert

   NpuC-C[AGGA]LFVY-NpuN-SsrA(rc)
   

   Expression

   Bacterial

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. SETD1A

   Plasmid

   #32868

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   SETD1A (SETD1A Human)
   

   Tags

   His tag (with TEV cleavage)

   Expression

   Bacterial

   Available Since

   Jan. 6, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pGEX4T-HEPN

    Plasmid

    #75296

    Purpose

    Expresses Sacsin HEPN domain (4441-4579) in fusion with GST (bacterial)

    Depositor

    Kalle Gehring

    Article

    Kozlov et al J Biol Chem. 2011 Jun 10;286(23):20407-1

    Insert

    Human Sacsin 4441-4579
    

    Tags

    GST-Tag

    Expression

    Bacterial

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKW08-Lx

    Plasmid

    #25012

    Depositor

    Brian Robertson

    Article

    Williams et al Plasmid. 2010 Sep;64(2):69-73. doi: 10.1

    Insert

    pKW08-Lx
    

    Expression

    Bacterial

    Available Since

    Aug. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET co-transformation cloning vector (13S-A)

    Plasmid

    #48323

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGTM_COV2_NSP5_C145A_001_SUMO

    Plasmid

    #192349

    Purpose

    Expresses SARS-CoV-2 3CLpro with an active site mutation (C154A) for the expression of the mature protease in an inactive form

    Depositor

    Gaetano Montelione

    Article

    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

    Insert

    SARS-COV-2 Mpro-C145A (NEWENTRY SARS-CoV-2)
    

    Tags

    6X-His-Sumo

    Expression

    Bacterial

    Mutation

    Cysteine 145 of the active site is substituted wi…

    Promoter

    T7 lac

    Available Since

    June 3, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pET PPL His6 LIC cloning vector (2J-T)

    Plasmid

    #37182

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6 and PPL

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFPV25-cR-2iG

    Plasmid

    #204618

    Purpose

    Fluorescent reporter for SPI2 induction in Salmonella

    Depositor

    Jacques Neefjes

    Article

    Stévenin et al Cell Rep. 2024 Nov 1;43(11):114931. doi:

    Inserts

    rpsM promoter
    sseA promoter
    

    Tags

    GFP and dsRed

    Expression

    Bacterial

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZE31-DsRed-ms2x2

    Plasmid

    #26248

    Depositor

    Philippe Cluzel

    Article

    Guet et al Nucleic Acids Res. 2008 Jul . 36(12):e73

    Insert

    ms2 binding sites (x2)
    

    Expression

    Bacterial

    Available Since

    Oct. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. Ki67 (LR)-mNeonGreen-IRESpuro2

    Plasmid

    #183743

    Purpose

    Expresses Ki67 (LR)-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 LR domain (MKI67 Human)
    

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    LR domain of Ki67

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHH0103_DNAJB1_DNAJ

    Plasmid

    #109918

    Purpose

    Protein expression and purification of DNAJB1_DNAJ

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    DNAJB1_DNAJ
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRS415-SPT6

    Plasmid

    #10993

    Depositor

    Francisco Malagon

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    Wild type

    Available Since

    Dec. 29, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBAD_IPF2.0

    Plasmid

    #163124

    Purpose

    A plasmid with IFP2.0 coding sequence coded into it. Expresses IFP2.0 with N-term His-tag.

    Depositor

    Julie Biteen

    Article

    Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

    Insert

    IFP2.0
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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