30,811 results

1. Terminase_T4

   Plasmid

   #188857

   Purpose

   Large terminase from phage T4

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

   Insert

   Terminase
   

   Expression

   Bacterial

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRDV5 containing Sb-convex

   Plasmid

   #132696

   Purpose

   pRDV5 plasmid containing a convex Sybody for the generation of a qPCR standard curve

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

   Insert

   Sb_convex including 5' and 3' flanking sequences used for ribosome display
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. 6xHis-MBP-TEV-mDvl2DEP(416-510)_pCDFduet

   Plasmid

   #216387

   Purpose

   Bacterial expression vector for MBP-tagged mouse Dvl2 DEP domain (residues 416-510)

   Depositor

   William Weis

   Article

   Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

   Insert

   Dishevelled-2 (Dvl2 Mouse)
   

   Tags

   6xHis-MBP-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMMBGent

   Plasmid

   #45475

   Depositor

   Michele Swanson

   Article

   Hammer et al Mol Microbiol. 1999 Aug;33(4):721-31.

   Insert

   Gentamycin
   

   Expression

   Bacterial

   Available Since

   June 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTXB1 EGFP

   Plasmid

   #129442

   Purpose

   expression of intein/ gfp

   Depositor

   Oskar Laur

   Insert

   intein/ gfp
   

   Expression

   Bacterial

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSK+ chickBMPR-1B DN

   Plasmid

   #49529

   Purpose

   cloning vector for dominant negative chick BMP receptor 1B

   Depositor

   Lee Niswander, Peter ten Dijke

   Article

   Zou et al Genes Dev. 1997 Sep 1;11(17):2191-203.

   Insert

   BMPR-1B DN (BMPR1B Chicken)
   

   Expression

   Bacterial

   Mutation

   dominant negative mutation (K231R); also P2A, M11…

   Promoter

   T3

   Available Since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pCF525-EF1a-Hygro-P2A-AcrVA5-lenti

   Plasmid

   #115664

   Purpose

   Expresses AcrVA5 in human cells, contained in a lentiviral vector.

   Depositor

   Jennifer Doudna

   Article

   Watters et al Science. 2018 Oct 12;362(6411):236-239.

   Insert

   AcrVA5
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Promoter

   EF-1a promoter

   Available Since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBAD-D4

   Plasmid

   #37473

   Depositor

   Amy Palmer, Roger Tsien

   Article

   Palmer et al Chem Biol. 2006 May;13(5):521-30.

   Insert

   D4 second generation cameleon (calcium sensor)
   

   Expression

   Bacterial

   Promoter

   Ara

   Available Since

   Oct. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

9. 14x PP7 with loxP-kanMX-loxP

   Plasmid

   #189939

   Purpose

   Integration of 14xPP7 loops in Saccharomyces cerevisiae. Selection by KanMX (flanked by LoxP sites for marker removal)

   Depositor

   Tineke Lenstra

   Article

   Donovan et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

   Insert

   14x PP7 with loxP-kanMX-loxP
   

   Tags

   kanMX

   Expression

   Bacterial

   Available Since

   Feb. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBBR6k-J23101-GFPuv

    Plasmid

    #106390

    Purpose

    pBBR6k-GFPuv with Anderson promoter J23101 expressing lacI

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Expression

    Bacterial

    Promoter

    LlacO-1

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHis-TEV-Nsp15

    Plasmid

    #187656

    Purpose

    E. coli plasmid (T7lac promoter) for full-length, wild-type SARS CoV-2 Nsp15 (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

    Depositor

    Debra Hansen

    Article

    Jernigan et al Structure. 2022 Dec 30:S0969-2126(22)004

    Insert

    Nsp15 (N Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    TEV protease cleavable His6-tag

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7lac

    Available Since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmSod1-G93A-N’His

    Plasmid

    #117705

    Purpose

    mutation on stability/solubility of SOD1

    Depositor

    Elizabeth Fisher

    Insert

    Sod1 (Sod1 Mouse)
    

    Tags

    His tag with Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    G93A

    Promoter

    T7

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. 10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-mGFP-StrepII (Human autophagy E3(mGFP)-like enzyme)

    Plasmid

    #169077

    Purpose

    Insect codon optimized ATG genes (excluded tags): 10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-GFP-TEV-StrepII

    Depositor

    Sascha Martens

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Inserts

    ATG5 (ATG5 Human)
    ATG7 (ATG7 Human)
    ATG10 (ATG10 Human)
    ATG12 (ATG12 Human)
    ATG16 (ATG16L1 Human)
    

    Tags

    GFP tag, GGlinker, TEV cleavage site. StrepII and…

    Expression

    Bacterial and Insect

    Promoter

    Polyhedrin promoter

    Available Since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28C-mCherry-YTHDF2

    Plasmid

    #204406

    Purpose

    E. coli expression of full-length YTHDF2 with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    YTHDF2 (YTHDF2 Human)
    

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28-Mff(1-61)-PP-GST

    Plasmid

    #73042

    Purpose

    Bacterial expression of mouse Mff residues 1-61 with C-terminal GST-tag and PreScission protease cleavage site

    Depositor

    David Chan

    Article

    Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77

    Insert

    Mff(1-61) (Mff Mouse)
    

    Tags

    GST-tag and PreScission protease site

    Expression

    Bacterial

    Mutation

    Truncation containing amino acids 1-61

    Promoter

    T7

    Available Since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPROEX Hsp104 (1-575)

    Plasmid

    #1318

    Depositor

    Susan Lindquist

    Article

    Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

    Insert

    Hsp104 (1-575) (HSP104 Budding Yeast)
    

    Tags

    6X His + TEV cleavage

    Expression

    Bacterial

    Mutation

    aa1-575 of Hsp104

    Available Since

    Jan. 21, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLAC

    Plasmid

    #45072

    Purpose

    lacritin-intein fusion plasmid coding for intact mature lacritin without signal peptide

    Depositor

    Gordon Laurie

    Article

    Wang et al J Cell Biol. 2006 Aug 28;174(5):689-700.

    Insert

    Lacritin (LACRT Human)
    

    Use

    Protein expression vector

    Tags

    Intein

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. P-BT3332

    Plasmid

    #188110

    Purpose

    pBolux harboring the promoter region upstream of BT3332

    Depositor

    Guy Townsend

    Article

    Modesto et al Nat Commun. 2023 Jan 17;14(1):275. doi:

    Insert

    The B. thetaiotaomicron BT3332 promoter cloned into pBolux
    

    Expression

    Bacterial

    Promoter

    BT3332

    Available Since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLK

    Plasmid

    #64186

    Purpose

    harbours marker genes LEU2 and LYS2

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    Aug. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSEVA221AID-T7RNAP

    Plasmid

    #167977

    Purpose

    Expresses fusion protein AID-T7RNAP in E. coli

    Depositor

    Luis Ángel Fernández

    Article

    Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

    Insert

    AID-T7RNAP
    

    Tags

    T7 tag and T7RNAP

    Expression

    Bacterial

    Promoter

    TetR-Ptet

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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