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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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34,238 results

Showing: 7801 - 7820 of 34238 results

  4. pRaGE Pyl TAG GFP Y35TAG

    Plasmid
    #160041
    Purpose
    Encodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter with TAG mutation at site 35, for unnatural amino acid incorporation into the GFP protein in Vibrio natriegens.
    Depositor
    Lital Alfonta
    Publication
    Ozer et al Front Bioeng Biotechnol. 2021 Feb 26;9:5
    Inserts
    Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
    Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
    deGFP
    Use
    Synthetic Biology
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed tyrosine 35 to TAG stop-codon (Site numbe…
    Promoter
    P70b promoter and T500 terminator, Vibrio natrieg…
    Available since
    May 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    160041-plasmid-map-sequence-id-332393

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  9. pBBR1-Rha-ETh1h2e_yi23-kan

    Plasmid
    #166670
    Purpose
    Genome engineering of Burkholderia
    Depositor
    Jun Fu
    Insert
    ETh1h2e_yi23
    Expression
    Bacterial
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    166670-plasmid-map-sequence-id-329556

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  10. Z-VCP-10

    Plasmid
    #166559
    Purpose
    scFv of a human scaffold targeting Transitional endoplasmic reticulum ATPase. Antigen coverage aa 1-806 of 806, full-length
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    166559-plasmid-map-sequence-id-329267

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  11. Q-ACACAA-10

    Plasmid
    #166546
    Purpose
    scFv of a human scaffold targeting Acetyl-CoA carboxylase 1. Antigen coverage aa 1572 - 2338 of 2346
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166546-plasmid-map-sequence-id-329238

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  20. pQE30-TEV-pro-conA

    Plasmid
    #159527
    Purpose
    Expresses pro-concanavalin A, a lectin from Canavalia ensiformis, in E. coli. Includes N-terminal TEV recognition site. Native N-terminal sequence is reconstituted upon TEV cleavage.
    Depositor
    Joshua Mylne
    Publication
    Nonis et al bioRxiv. 2020.
    Insert
    Canavalia ensiformis pro-concanavalin A
    Tags
    Six-Histidine tag and tobacco etch virus (TEV) pr…
    Expression
    Bacterial
    Promoter
    T5
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    159527-plasmid-map-sequence-id-317076

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Showing: 7801 - 7820 of 34238 results