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31,827 results

Showing: 7841 - 7860 of 31827 results

  2. pTO_CHMP2B_LAP

    Plasmid
    #101848
    Purpose
    Tet-inducible expression of human CHMP2B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Article
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    CHMP2B (CHMP2B Human)
    Use
    Flp-frt recombination, tet-inducible expression
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Mutation
    silent mutation in Thr 104; A to T
    Promoter
    CMV
    Available Since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    101848-plasmid-map-sequence-id-200235

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  4. pETM-30-GST-PfAgo_F

    Plasmid
    #146265
    Purpose
    Bacterial Expression of PfAgo
    Depositor
    Elisa Izaurralde
    Insert
    PfAgo
    Expression
    Bacterial
    Mutation
    one silent T1704C and one non silent mutation A90…
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146265-plasmid-map-sequence-id-456974

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  5. PCRT7 tetR pLtetO MBP-MEK1 XX Amp

    Plasmid
    #53225
    Purpose
    human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis
    Depositor
    Jesse Rinehart
    Insert
    MEK1 (MAP2K1 Human)
    Use
    Phosphoprotein synthesis
    Tags
    HIS and MBP
    Expression
    Bacterial
    Mutation
    Codon optimized for E.coli, both codons for Ser21…
    Promoter
    pLtetO
    Available Since
    May 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    53225-plasmid-map-sequence-id-82901

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  11. ibpAB-GFP

    Plasmid
    #109390
    Purpose
    GFP cassette under the control of the ibpAB sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.
    Depositor
    Tom Ellis
    Article
    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463
    Insert
    pibpAB-GFP
    Expression
    Bacterial
    Available Since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    109390-plasmid-map-sequence-id-210782

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  18. pETSUMO2_Vitiosangium-bGSDM-thrombin

    Plasmid
    #222099
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Vitiosangium-bGSDM-thrombin ORF
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222099-plasmid-map-sequence-id-437094

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  19. pETSUMO2_Nostoc-bGSDM-WELQ

    Plasmid
    #222100
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Nostoc species with a WELQut protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Nostoc-bGSDM-WELQ ORF
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222100-plasmid-map-sequence-id-437100

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  20. pQ-Elo-HB-C-A(1-630)

    Plasmid
    #171086
    Purpose
    Co-expresses of ΔC-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    Elongin B (ELOB Human)
    Elongin C (ELOC Human)
    Elongin A (ELOA Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Deleted N-term 26aa and C-term 142aa for mutant e…
    Promoter
    T5 promoter
    Available Since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171086-plasmid-map-sequence-id-335555

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Showing: 7841 - 7860 of 31827 results