32,453 results
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Plasmid#35357DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialPromoterLlacO-1Available SinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSL0915 (CRISPR with target 3)
Plasmid#130643PurposeExpresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA that targets lacZ.DepositorInsertCRISPR(target-3)
UseCRISPR; TransposonExpressionBacterialPromoterT7Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
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Nck
Plasmid#186133PurposeFor expression of recombinant Nck in bacteria cells.DepositorInsertNCK1 (NCK1 Human)
TagsGSTExpressionBacterialMutationAmino acid residues 1-377, with mutations: C139S,…PromoterLacAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-21a_2xNLS_LbCpf1_protein_expression
Plasmid#114366PurposeProtein expression plasmid for 2xNLS LbCpf1 in pET-21a backboneDepositorInsert2xNLS_LbCpf1
UseCRISPRTagsNLSExpressionBacterialPromoterT7Available SinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCsy4
Plasmid#44252DepositorInsertcsy4 gene
UseSynthetic BiologyExpressionBacterialPromoterpLtetO-1Available SinceApril 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pgex-cIAP1 mut
Plasmid#8334DepositorAvailable SinceNov. 15, 2005AvailabilityAcademic Institutions and Nonprofits only -
pBS mPax1 (CT#1007)
Plasmid#13898DepositorInsertPax1 (Pax1 Mouse)
ExpressionBacterialAvailable SinceFeb. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
BBa_J72113-BBa_J72152
Plasmid#40784DepositorInsertPhagemid + GFP
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTXBI-mclover-YTHDC1-FL
Plasmid#177121PurposeBacterial expression of Full-length YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purificationDepositorInsertYTHDC1 (YTHDC1 Human)
TagsMxe GyrA intein-Chitin binding domain for protein…ExpressionBacterialPromoterT7Available SinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKERep
Plasmid#211826PurposeMedium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.DepositorInserteGFP
ExpressionBacterialPromoterT5Available SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKESa
Plasmid#211828PurposeLow copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.DepositorInserteGFP
ExpressionBacterialPromoterT5Available SinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
AURKA_HUMAN_D0
Plasmid#79739PurposeThis plasmid encodes the kinase domain of AURKA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAR127_StrepI_m(KS_MAT)_mouseL_ACP_H8_pET22b
Plasmid#122850PurposeExpresses a designed construct of the condensing part fused to the ACP domain of murine type I fatty acid synthase (FASN) in Escherichia coliDepositorInsertm(KS_MAT)_mouseL_ACP (Fasn Mouse)
TagsHis8 tag and Twin-Strep tagExpressionBacterialMutationwildtypePromoterT7 promoterAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZE21/UBP1/ClpS_V65I
Plasmid#98567PurposeExpresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operonDepositorInsertsTruncated ubiquitin cleavase, codon optimized
Mutated ATP-dependent Clp protease adapter protein
ExpressionBacterialMutationContains the V65I mutation that increases discrim…Available SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBSM
Plasmid#67505PurposeExpress the tRNAMet scaffoldDepositorInsertmodified tRNAMet
ExpressionBacterialPromoterlppAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNR276
Plasmid#79577PurposeTP901 attB-attP. GFP will express if sites recombine.DepositorInsertGFPmut3b
ExpressionBacterialAvailable SinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNR239
Plasmid#79576PurposeA118 attB-attP. GFP will express if sites recombine.DepositorInsertGFPmut3b
ExpressionBacterialAvailable SinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-Sac-Abeta(MC1-40)
Plasmid#127152PurposeExpresses N-terminal cysteine Abeta(1-40)DepositorAvailable SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSU315
Plasmid#169697PurposeTemplate plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert1xHA epitope tag adjacent to FRT-flanked aph (KanR) gene
Tags1xHA tagExpressionBacterialAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only