30,235 results

1. pPro24(s)-gfp

   Plasmid

   #17813

   Depositor

   Jay Keasling

   Article

   Lee et al Biotechnol Bioeng. 2006 Apr 5. 93(5):912

   Insert

   GFPuv
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   D234N compared to AF007834

   Promoter

   Available since

   May 16, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pTriEx-mVenus-Zdk1-VAV2 DH/PH/C1

   Plasmid

   #81133

   Purpose

   mammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domain

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Inserts

   Zdk1
   VAV2 DH/PH/C1 (VAV2 Human)
   

   Use

   Tags

   mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCORE-Kp53

   Plasmid

   #72241

   Purpose

   Contains the CORE cassette with KanMX4 and human p53 gene markers

   Depositor

   Francesca Storici, Michael Resnick

   Article

   Storici et al Genet Eng (N Y). 2003;25:189-207.

   Inserts

   p53 (TP53 Human)
   kanMX4
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V122A mutation

   Promoter

   Available since

   Jan. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. KDM4D

   Plasmid

   #73247

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   KDM4D (KDM4D Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   pfam02375:JmjN 18-64, pfam02373:JmjC 182-295

   Promoter

   T7

   Available since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKS101

   Plasmid

   #24631

   Depositor

   Jay Keasling

   Article

   Steen et al Nature. 2010 Jan 28;463(7280):559-62.

   Insert

   FAA2 (FAA2 Budding Yeast)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pKan cpRBP-FL P53

   Plasmid

   #174504

   Purpose

   Express Full-length P53 in E.coli with cleavable expression/purification tag (ribose binding protein circular permutant)

   Depositor

   Stewart Loh

   Article

   Ha et al Bio Protoc. 2021 Oct 20;11(20):e4188. do

   Insert

   P53 gene (TP53 Human)
   

   Use

   Tags

   20 amino acid linker containing HRV3C protease si…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTC-mcs

   Plasmid

   #20317

   Depositor

   Dirk Schnappinger

   Article

   Klotzsche et al Nucleic Acids Res. 2009 Apr;37(6):1778-8

   Insert

   multiple cloning site
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBbE0a-RFP

   Plasmid

   #35372

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNP1 sfGFP trigger

   Plasmid

   #107359

   Purpose

   Trigger sequence for pNP1 sfGFP toehold switch

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   toehold trigger 7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOPS0750

    Plasmid

    #115511

    Purpose

    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing gusA.

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    gusA pOGG083, T-pharma pOGG003 assembled in pOGG026
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Constitutive promoter pNeo pOGG001

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX3x-hRanBP2-(IR1+M)

    Plasmid

    #53140

    Purpose

    E.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activity

    Depositor

    Frauke Melchior

    Article

    Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

    Insert

    RanBP2 fragment (RANBP2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    fragment (amino acids 2631-2711)

    Promoter

    tac

    Available since

    May 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGBHPS-SortA

    Plasmid

    #21930

    Depositor

    Fuyuhiko Inagaki

    Article

    Kobashigawa et al J Biomol NMR. 2009 Mar . 43(3):145-50.

    Insert

    sortase
    

    Use

    Tags

    GB1, HRV3C cleagage site, and His

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59

    Promoter

    Available since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-KIX (586-672)

    Plasmid

    #99336

    Purpose

    Bacterial expression of mouse CBP KIX domain

    Depositor

    Peter Wright

    Article

    Radhakrishnan et al J Mol Biol. 1999 Apr 16;287(5):859-65.

    Insert

    CBP KIX (586-672) (Crebbp Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p7XC3H

    Plasmid

    #47065

    Purpose

    FX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site and 10x His tag

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site) and decaHis (…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKB226

    Plasmid

    #170016

    Purpose

    Encodes LacI-controlled expression of surface-displayed defensin HNP-1 and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    Defensin HNP-1
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and PvirK

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVE2_11_p40S_QfDBD_VP16AD_cp_phl_DsRed_0xQUAS

    Plasmid

    #154229

    Purpose

    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_phl (no QUAS control)

    Depositor

    Arnold Driessen

    Article

    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.

    Insert

    p40S:QfDBD-VP16AD-GFP-NLS
    

    Use

    Synthetic Biology; Fungal expression

    Tags

    GFP-NLS; DsRed-SKL

    Expression

    Bacterial

    Mutation

    Promoter

    p40S (AN0465 A. nidulans)

    Available since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. S5_EcoRV_Ef1a_AscI

    Plasmid

    #128469

    Purpose

    Ef1a promoter

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    Ef1a promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-MinC

    Plasmid

    #133628

    Purpose

    Expression vector for the Escherichia coli MinC with an N-terminal fusion of a His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Loose et al Nat Struct Mol Biol. 2011 May;18(5):577-

    Insert

    His-MinC (minC E. coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pH6-spTorA-mCherry

    Plasmid

    #169040

    Purpose

    mCherry preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag in pET28a.

    Depositor

    Siegfried Musser

    Article

    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

    Insert

    H6-spTorA-mCherry
    

    Use

    Tags

    6xHis and spTorA (signal peptide of E. coli TorA)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBS mNFATc3-EE

    Plasmid

    #17868

    Depositor

    Jerry Crabtree

    Article

    Graef et al Cell. 2001 Jun 29. 105(7):863-75.

    Insert

    NFATc3 (Nfatc3 Mouse)
    

    Use

    Tags

    EE

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only