30,875 results

1. pGEX-4T-1-RIPK3

   Plasmid

   #78827

   Purpose

   To purify GST-tagged recombinant RIPK3 protein

   Depositor

   Jaewhan Song

   Article

   Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

   Insert

   RIPK3 (RIPK3 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMYO127TAG-SepT

   Plasmid

   #34625

   Depositor

   Jesse Rinehart, Dieter Söll

   Article

   Park et al Science. 2011 Aug 26;333(6046):1151-4.

   Insert

   Myoglobin-127TAG
   

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   codon-optimized + an amber stop codon was introdu…

   Promoter

   Ipp

   Available Since

   Feb. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEM4Z-mMcl-1

   Plasmid

   #32984

   Depositor

   Joseph Opferman

   Article

   Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

   Insert

   Mcl-1 (Mcl1 Mouse)
   

   Expression

   Bacterial

   Available Since

   March 15, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pTn7·X-nia

   Plasmid

   #122592

   Purpose

   Plasmid for genomic integraction of xylS-Pm->nia into the Tn7 insertion site

   Depositor

   Pablo Ivan Nikel

   Article

   Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

   Insert

   xylS/Pm->Nia
   

   Use

   Synthetic Biology; Tn7 genomic integration

   Expression

   Bacterial

   Promoter

   Pm

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBBR1-kan-Ptet-plu-gamma-ETh_rhi483

   Plasmid

   #180429

   Purpose

   Genome engineering of Agrobacterium

   Depositor

   Jun Fu

   Article

   Bian et al Appl Environ Microbiol. 2022 Mar 8;88(5)

   Insert

   plu-gamma-ETh_rhi483
   

   Expression

   Bacterial

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBC155

   Plasmid

   #202274

   Purpose

   Level 2 for strain-specific barcode DNA tag with bc182 through Tn7 bacterial insertion, Spectinomycin and Streptomycin selectable marker and tagBFP fluorescent marker

   Depositor

   Paul Schulze-Lefert

   Article

   Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

   Insert

   bc182
   

   Expression

   Bacterial

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBC114

   Plasmid

   #202276

   Purpose

   Level 2 for strain-specific barcode DNA tag with bc141 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum

   Depositor

   Paul Schulze-Lefert

   Article

   Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

   Insert

   bc141
   

   Expression

   Bacterial

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-6p-1_GST-DmKHC[1-421]_1xmNeonGreen

   Plasmid

   #196974

   Purpose

   Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal mNeonGreen tag and cleavable N-terminal GST tag

   Depositor

   Lukas Kapitein

   Article

   Chen et al Elife. 2022 Jul 5;11:e77892. doi: 10.755

   Insert

   Kinesin Heavy Chain (Khc Fly)
   

   Tags

   GST and mNeonGreen

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDSG287

   Plasmid

   #115606

   Purpose

   surface-displayed Ag2, used in composability experiments; low copy

   Depositor

   Ingmar Riedel-Kruse

   Article

   Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

   Insert

   regulated adhesin construct
   

   Expression

   Bacterial

   Available Since

   Oct. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJKR-O-acuR

    Plasmid

    #62569

    Purpose

    Acrylate biosensor. Medium copy plasmid that expresses CFP proportional to acrylate concentration. Compatible with all other pJKR plasmids.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    CFP
    

    Expression

    Bacterial

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSaPIPLC His

    Plasmid

    #173810

    Purpose

    Expression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cells

    Depositor

    Anne Gershenson, Mary F Roberts

    Article

    Goldstein et al Biochemistry. 2012 Mar 27;51(12):2579-87

    Insert

    Staphylococcus aureus plc
    

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOPINO_H3

    Plasmid

    #171927

    Purpose

    Bacterial expression of nanobody VHH_H3, which binds to receptor binding domain of the spike protein of SARS-CoV-2

    Depositor

    Ray Owens

    Article

    Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

    Insert

    VHH_H3
    

    Tags

    His6

    Expression

    Bacterial and Mammalian

    Available Since

    Sept. 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pATZ-BFP-HA-GFP11

    Plasmid

    #177721

    Purpose

    ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFP

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    alpha-1-antitrypsin (SERPINA1 Human)
    

    Tags

    GFP11, HA, and TagBFP

    Expression

    Bacterial and Mammalian

    Mutation

    E342K (ATZ mutant)

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX NBP1 (P#415)

    Plasmid

    #14658

    Depositor

    Matija Peterlin

    Article

    Lu et al Immunity. 1998 May . 8(5):647-56.

    Insert

    ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H Human)
    

    Expression

    Bacterial

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pQE30-rSyntenin1

    Plasmid

    #89428

    Purpose

    bacterial expression of rat Syntenin1

    Depositor

    Fritz Rathjen

    Insert

    SDCBP
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. dCas9-CRY2Clust-FUSN-mut

    Plasmid

    #200970

    Purpose

    induction of phase-separated condensate mediated by FUSN-IDR mutant

    Depositor

    Jian Xu

    Article

    Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

    Insert

    FUSNmut
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Mutation

    15 Tyr-to-Ser substitutions

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-5X-1 U1-C-FL

    Plasmid

    #129077

    Purpose

    generation of GST-tagged U1-C protein using bacteria

    Depositor

    Takeshi Tsubata

    Insert

    U1-C (SNRPC Human)
    

    Expression

    Bacterial

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET3a-Ub-K48C

    Plasmid

    #18897

    Depositor

    Cecile Pickart

    Article

    Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1

    Insert

    Ubiquitin-K48C (UBC Human)
    

    Expression

    Bacterial

    Mutation

    Lysine 48 is replaced with a Cysteine residue

    Available Since

    Sept. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pET51b_SNAP-ecDHFR

    Plasmid

    #187108

    Purpose

    Expression of SNAP-ecDHFR fusion in bacteria

    Depositor

    Kai Johnsson

    Article

    Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

    Insert

    SNAP-ecDHFR
    

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pShHELIX(Cas12k-TniQ)-sgRNA_entry (CJT111)

    Plasmid

    #181790

    Purpose

    Expresses 3-component ShHELIX containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ
    

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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