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pBDK

Plasmid

#135190

Purpose

Integration and excision of kanamycin cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection

Depositor

Hongqing Fang

Article

Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

Insert

KanR

Use

Synthetic Biology

Expression

Bacterial

Promoter

cat

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pSiMPla_N + pSiMPla_C

Plasmid

#134314

Purpose

Maintenance of 2 plasmids with a single antibiotic in E. coli

Depositor

Barbara Di Ventura

Article

Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

Insert

ampR_gp41-1 N-intein + ampR_gp41-1 C-intein

Expression

Bacterial

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pSiMPlh_N + pSiMPlh_C

Plasmid

#134316

Purpose

Maintenance of 2 plasmids with a single antibiotic in E. coli

Depositor

Barbara Di Ventura

Article

Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

Insert

hygR_gp41-1 N-intein + hygR_gp41-1 C-intein

Expression

Bacterial

Mutation

P254Q in hygR_gp41-1 N-intein

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pNCST-AvicFP2

Plasmid

#129505

Purpose

Expresses AvicFP2 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AvicFP2

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pNCST-AvicFP4

Plasmid

#129507

Purpose

Expresses AvicFP4 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AvicFP4

Expression

Bacterial

Mutation

One of two variants of AvicFP4 tested with essent…

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL7004

Plasmid

#136943

Purpose

Malachite green aptamer, T7 promoter

Depositor

Julius Lucks

Article

Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

Insert

malachite green aptamer (MGA)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pT7

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL7005

Plasmid

#136944

Purpose

Malachite green aptamer with stability hairpin, T7 promoter

Depositor

Julius Lucks

Article

Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

Insert

Malachite green aptamer

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pJ211_WT_low

Plasmid

#128848

Purpose

Low copy number plasmid for expression of E. coli flagellin from its natural promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pNCST-AausFP3

Plasmid

#129501

Purpose

Expresses AausFP3 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AausFP3

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pTW195b63

Plasmid

#136931

Purpose

Directed evolution of SpCas11

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

gIII(N)-npuN

Use

CRISPR

Expression

Bacterial

Mutation

none

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pJS183

Plasmid

#134142

Purpose

Bacterial expression of GFP-Ska1 and Ska2

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-GFP-human Ska1; human Ska2

Tags

GFP

Expression

Bacterial

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pJOE6261.2

Plasmid

#135079

Purpose

P. putida KT2440 rescue construct

Depositor

Josef Altenbuchner

Article

Graf et al Appl Environ Microbiol. 2011 Aug;77(15):

Insert

upp (upp Pseudomonas putida)

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-mCherryGGBsMTS

Plasmid

#133625

Purpose

Expression vector for mCherry fused to a membrane targeting sequence (MTS) of the B.subtilis MinD (C-terminal fusion) and an N-terminal His-tag for purification.

Depositor

Petra Schwille

Article

Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

Insert

mCherryGGBsMTS

Expression

Bacterial

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLB038 - PHS1∆P

Plasmid

#136285

Purpose

entry vector for GreenGate cloning method, C module, open reading frame of phosphatase-inactive PROPYZAMIDE-HYPERSENSITIVE 1 (PHS1) (PHS1DP)

Depositor

Alexis Maizel

Article

Vilches Barro et al Curr Biol. 2019 Aug 5;29(15):2443-2454.e

Insert

PHS1∆P

Expression

Bacterial

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-3xMyc

Plasmid

#129569

Purpose

Empty backbone for Myc Tag

Depositor

Kevin Janes

Article

Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a His6Flag2AUF1(p45)

Plasmid

#135942

Purpose

Expresses AUF1p45 in E. coli

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Tags

Flag2 and His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pFUM

Plasmid

#133130

Purpose

A recombinant plasmid expressing fum (fumarase) gene

Depositor

Gregg Beckham

Article

Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

fumarase gene

Expression

Bacterial

Promoter

PEP-carboxykinase promoter

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-PP-Spastin(87-616)DeltaExon4

Plasmid

#128794

Purpose

Bacteiral expression of Spastin M87 varient without exon 4

Depositor

Chris Hill

Article

Han et al J Biol Chem. 2019 Nov 25. pii: AC119.009

Insert

Spastin (SPAST Human)

Use

`

Tags

GST

Expression

Bacterial

Mutation

residues 87-616 without exon 4.

Promoter

T7

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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Omega 1

Plasmid

#118046

Purpose

GoldenBraid2.0 Omega 1 destination vector

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Type

Empty backbone

Use

Synthetic Biology; Assembly of gb2.0 transcriptio…

Expression

Bacterial, Insect, and Mamm…

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pDM_noro_A2_lacZA

Plasmid

#118819

Purpose

T7 RNAP-driven expression of norovirus antisense orientation toehold switches (A2) with a lacZ alpha subunit reporter.

Depositor

Alexander Green

Article

Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

Insert

Norovirus toehold switch A2

Expression

Bacterial

Available Since

Nov. 27, 2019

Availability

Academic Institutions and Nonprofits only

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