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ETV1_pet28a

Plasmid

#131644

Purpose

Bacterial expression of ETS gene ETV1

Depositor

Peter Hollenhorst

Publication

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV1 (ETV1 Human)

Tags

6xHis

Expression

Bacterial

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pSG84-pLas-TetRssrA

Plasmid

#102455

Purpose

Expresses TetR protein with ssrA degradation tag. The expression is controlled by a pLas promoter.

Depositor

Richard Murray

Publication

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

TetR (tetR )

Tags

ssrA

Expression

Bacterial

Promoter

pLas

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pHybr_r2a>mG1-srt-his

Plasmid

#124802

Purpose

HDR template to knock-in mouse IgG1 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG1 producing cell lines.

Depositor

Ferenc Scheeren, Martijn Verdoes

Publication

van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

Insert

Murine IgG1

Use

CRISPR; Hdr template

Tags

Histag (HHHHHH) and Sortag (LPETGG)

Expression

Bacterial and Mammalian

Promoter

-

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMOD3-proD/mNeptune2-nat

Plasmid

#120337

Purpose

Bacterial mNeptune2 labeling

Depositor

James Kirby

Publication

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mNeptune2

Expression

Bacterial

Promoter

proD

Available since

Aug. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pTAMAHISTEV_BauA

Plasmid

#128952

Purpose

expresses BauA in E.coli

Depositor

James Naismith

Publication

Moynie et al Elife. 2018 Dec 18;7. pii: 42270. doi: 1

Insert

ferric acinetobactin receptor

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-66)

Promoter

T7

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAC572

Plasmid

#127973

Purpose

AMA1 plasmid with pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)

Depositor

Uffe Mortensen

Publication

Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

Insert

pyrG

Use

Fungal expression

Expression

Bacterial

Promoter

native promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pASR102

Plasmid

#122636

Purpose

BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae (pPEPZ Backbone)

Depositor

Jan-Willem Veening

Publication

Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pWJB9

Plasmid

#126650

Purpose

Expresses AdrA from a modified pLAFR3

Depositor

Ute Romling

Publication

Simm et al Mol Microbiol. 2004 Aug;53(4):1123-34. d

Insert

adrA-8xHis/AdrA-8xHis including flanking promoter regions from pBAD30

Tags

8xHis

Expression

Bacterial

Promoter

PBAD

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5099

Plasmid

#128714

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Publication

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopK1

Expression

Bacterial

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5616

Plasmid

#128727

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Publication

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

avrPtoB

Expression

Bacterial

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5328

Plasmid

#128721

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Publication

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopU1

Expression

Bacterial

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5306

Plasmid

#128712

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Publication

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopH1

Expression

Bacterial

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5571

Plasmid

#128713

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Publication

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopI1

Expression

Bacterial

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5539

Plasmid

#128728

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Publication

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopAF1

Expression

Bacterial

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5618

Plasmid

#128709

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Publication

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopE1

Expression

Bacterial

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP6534

Plasmid

#128735

Purpose

Gateway destination pUC18R6KT-Tn7T-PavrPto-GW-Cya-Km vector for integration into DC3000D36E genome glmS-linked attTn7 site any effector gene, expressed from avrPto promoter and with C-terminal HA tag

Depositor

Alan Collmer

Publication

Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

Type

Empty backbone

Expression

Bacterial

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5617

Plasmid

#128706

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Publication

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopB1

Expression

Bacterial

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pET-His6-Sumo-IntNtr-eSrt2A

Plasmid

#126015

Purpose

Bacterial expression of IntNtr-eSrt2A fusion protein (with N-terminal His6-Sumo)

Depositor

Thomas Muir

Insert

His6-Sumo-IntNtr-eSrt2A

Tags

6xHis-SUMO

Expression

Bacterial

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pNIC28-BSA4-G-L11

Plasmid

#127830

Purpose

Production of recombinant human ribosomal protein L11

Depositor

Ivanhoe Leung

Publication

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPL11 (RPL11 Human)

Expression

Bacterial

Promoter

T7

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT-G-L11

Plasmid

#127831

Purpose

Production of recombinant human ribosomal protein L11

Depositor

Ivanhoe Leung

Publication

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPL11 (RPL11 Human)

Tags

His6 - Thioredoxin - TEV

Expression

Bacterial

Promoter

T7

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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