32,457 results
-
Plasmid#111489Purposetranslational elongation factor GDepositorInserttranslational elongation factor G
Tags6xHisExpressionBacterialAvailable SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b_pScaC-SNAP_6xHis
Plasmid#228835PurposeE. coli expression of the SNAP-tagged ScaC passenger domainDepositorInsertScaC
TagsSNAPtag-TEV-6xHisExpressionBacterialMutationaa 33-223Available SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
MT1X
Plasmid#105709PurposeBacterial expression plasmids for production of recombinant metallothionein 1xDepositorAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
MT1F
Plasmid#105703PurposeBacterial expression plasmids for production of recombinant metallothionein 1fDepositorAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delCAT
Plasmid#173942PurposeBacterial expression of PARP1 lacking CAT domainDepositorInsertPARP1delCAT (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 661-1014Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC SNAP-KDEL
Plasmid#104982PurposeLentiviral vector expressing SNAP-KDEL for ER labelingDepositorInsertSNAP-KDEL
UseLentiviralTagsSecretion signalExpressionBacterial and MammalianPromoterUbiCAvailable SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
LACTB2
Plasmid#39115PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCX0393
Plasmid#205984PurposeGa0116245_10002768 T7/LacO-Cas6 + Cas14-TwinStrep + Cas7 + His14-Cas5::pJ23119-truncated DR-truncated Fn spacerDepositorInsertsGa0116245_10002768 candidate type VII proteins
Cas14
Cas5
truncated CRISPR array
Tags14xHis and TwinStrep-tagExpressionBacterialPromoterT7 (proteins), pJ23119 (crRNA)Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-PA-Rac1-C450A
Plasmid#22025PurposeExpression of Rac1 fused with light-insensitive LOV2 (C450A mutation)DepositorInsertPA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
Tags6X HisExpressionBacterial, Insect, and Mamm…MutationLOV domain contains light-insensitive mutation C4…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRS415-SPT6
Plasmid#10993DepositorAvailable SinceDec. 29, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PGC1(1-400)
Plasmid#1029DepositorAvailable SinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-PA-Rac1-I539E
Plasmid#22026PurposeExpression of photoactivatable Rac1 ('lit' state mutant)DepositorInsertPA-Rac1-I539E (RAC1 Avena sativa (oat), Human)
Tags6X HisExpressionBacterial, Insect, and Mamm…MutationLOV domain contains 'lit state' mutatio…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Tipin-His
Plasmid#23122DepositorAvailable SinceJan. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDule2-IBBN (G2)
Plasmid#85501PurposePlasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertIBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32G L65E F108W Q109M D158S R162KPromoterlpp (constitutive)Available SinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTW195b63
Plasmid#136931PurposeDirected evolution of SpCas11DepositorInsertgIII(N)-npuN
UseCRISPRExpressionBacterialMutationnoneAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
HS_NAM-B1
Plasmid#154064PurposeLevel M, P1 Golden Gate vector. OsAct::Hyg-nosT/HvHSP17::Cre-hspT/ZmUbi::loxP-GUS-loxP-NAM-B1-nosTDepositorInsertsHygromycin phosphotransferase
Cre recombinase
LoxP-flanked GUS and TtNAM-B1
UseCre/Lox and Synthetic Biology; Golden gateExpressionBacterial and PlantMutationThe TtNAM-B1 gene sequence was domesticated to re…PromoterHvHSP17, OsAct, and ZmUbiAvailable SinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
ELK3_pet28a
Plasmid#131651PurposeBacterial expression of ETS gene ELK3DepositorAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pE4c
Plasmid#17464Purposemodified pENTR vector for C-terminal GST tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsGSTExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJ211_WT_low
Plasmid#128848PurposeLow copy number plasmid for expression of E. coli flagellin from its natural promoterDepositorInsertFlagellin
ExpressionBacterialAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
P48
Plasmid#212155PurposePlasmid expressing 6x His tagged secondary alcohol dehydrogenase fused with superfolder green fluorescent protein at N terminus under constitutive promoter PgyrA control.DepositorInsertsuperfolder green fluorescent protein, secondary alcohol dehydrogenase
Tags6x HisExpressionBacterialPromoterPgyrAAvailable SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only