31,828 results
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Plasmid#101239Purposewildtype Fgß peptide with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forcesDepositorInsertFgß peptide, ddFLN4 fingerprint (FGB D. discoideum, Human)
TagsHIS and ybbrExpressionBacterialMutationC18S in ddFLN4PromoterT7Available SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET303-ColE1-T-E192C
Plasmid#180233PurposeExpresses T domain of Colicin E1 (residues 1-190) with C terminal cysteine for maleimide chemistryDepositorInsertColicin E1
Tags6x His-tagExpressionBacterialMutationTruncation of ColE1 containing T domains (residue…PromoterT7Available SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
TrpLE-M-nMSP1E3D1
Plasmid#179949PurposeTagless membrane scaffold protein (MSP) for formation of nanodiscs.DepositorInsertTrpLE-M-nMSP1E3D1
TagsTrpLEExpressionBacterialMutationNative Trp residues have been mutated to Phe resi…PromoterT7Available SinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK047
Plasmid#65154PurposeEncodes GFP dropout as a Type 234r part to be used in the Dueber YTK systemDepositorInsertGFP dropout
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
GSG2
Plasmid#38915PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJB158
Plasmid#202601PurposeRP4-based mobilizable plasmid for the IPTG inducible expression of inserts that can be introduced using BsaI-based Golden Gate cloning. Vector has two origins of replication.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSNAP-CAPZ
Plasmid#69948PurposeSimultaneous expression of a His- and SNAP-tagged β1 subunit and untagged α1 subunits of chicken CapZDepositorTagsHis 6 and SNAPExpressionBacterialAvailable SinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAcCAST-sgRNA_entry (CJT83)
Plasmid#181785PurposeExpresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertAcTnsB, AcTnsC, AcTniQ, AcCas12k
ExpressionBacterialPromoterLac and J23119Available SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
RNF168
Plasmid#25298PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 16, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBbB5a-GFP
Plasmid#35352DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialPromoterlacUV5Available SinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSK+ chickBMPR-1B DN
Plasmid#49529Purposecloning vector for dominant negative chick BMP receptor 1BDepositorInsertBMPR-1B DN (BMPR1B Chicken)
ExpressionBacterialMutationdominant negative mutation (K231R); also P2A, M11…PromoterT3Available SinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pE3c
Plasmid#17463Purposemodified pENTR vector for C-terminal 6x MYC tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTags6xMycExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pNCST-mAvicFP1
Plasmid#129509PurposeExpresses mAvicFP1 constitutively in E. coli (most strains)DepositorInsertmAvicFP1
ExpressionBacterialPromotersynthetic constitutive (stationary phase) promote…Available SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
BRD1
Plasmid#39095PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
137 pGEX2TK RBAP2 HA
Plasmid#11174DepositorAvailable SinceJan. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET30b_T7Sav
Plasmid#138588PurposeT7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 oriDepositorInsertT7-tagged streptavidin variant with native C-terminus
UseSynthetic BiologyTagsT7-tagExpressionBacterialPromoterPT7Available SinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCON1-ProB.mCherry
Plasmid#112520PurposeProB driven mCherry expression with synTTDepositorInsertProB mCherry TT
ExpressionBacterialPromoterProBAvailable SinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSUMO-LIC-CHIKV-nsP2h
Plasmid#127935PurposeExpresses CHIKV nsP2h (1-465 aa) in bacterial cellsDepositorInsertCHIKV nsP2h
TagsHis6-Sumo-TEVExpressionBacterialPromoterT7 promoterAvailable SinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c028
Plasmid#139765PurposeProtein expression/Streptavidin pull-downDepositorInsertTBXT, Full-length, WT (TBXT Human)
TagsAvi-tag (Biotin) and His6-GST-TEVExpressionBacterialMutationContains only amino acids S2- M435PromoterT7Available SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-2xTS
Plasmid#109419PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
TagsBAP, HA, His6, T7, and Tyrosine sulfation (TS) se…ExpressionBacterialPromoterT7Available SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only