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4A3.PBAD-repL(AT2).J23115-gfp

Plasmid

#197873

Purpose

Arabinose induced expression of repL(AT2) gene, which triggers DNA replication in trans, weak level of constitutive GFP expression

Depositor

Baojun Wang

Article

Huan et al Front Microbiol. 2023 Feb 9;14:1095671.

Insert

part kilA/repL(AT2)

Expression

Bacterial

Promoter

PBAD

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pSUMO His6 SUMO SnRK2.6 (11-362)

Plasmid

#177847

Purpose

Bacterial Expression of SnRK2.6

Depositor

Karsten Melcher

Article

Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

Insert

SNF1-related protein kinase 2.6 (OST1 Mustard Weed)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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hsp70: mKate2-DN Kif5a

Plasmid

#105972

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

Insert

Kif5a (kif5aa Zebrafish)

Tags

mKate2

Expression

Bacterial

Mutation

truncation of aa416-1033

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pMS30: pTarget(PmcCAST)

Plasmid

#168163

Purpose

Target plasmid containing PmcPSP1 and attachment site for PmcCAST.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PAM for PmcCAST + PmcPSP1
tRNA-Val

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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dCas9-CRY2Clust-FUSN-mut

Plasmid

#200970

Purpose

induction of phase-separated condensate mediated by FUSN-IDR mutant

Depositor

Jian Xu

Article

Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

Insert

FUSNmut

Use

Lentiviral

Expression

Bacterial and Mammalian

Mutation

15 Tyr-to-Ser substitutions

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pFS_0271_pET30_pCat-tetR-Term-ptetA-sfGFP-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

Plasmid

#116955

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac and sfGFP under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCPP5951

Plasmid

#128719

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi

Insert

hopR1

Expression

Bacterial

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR R4 ATCC LacZ GCTT

Plasmid

#173668

Purpose

Destination vector for Golden Gate Cloning assemblies, blue-white screening

Depositor

Eric Marois

Article

Green et al Elife. 2023 Dec 5;12:e93142. doi: 10.755

Type

Empty backbone

Expression

Bacterial and Insect

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-6p-1 Sesn2

Plasmid

#61873

Purpose

bacterial expression of a GST-Sesn2 (mouse)

Depositor

Ming Li

Article

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn2 (Sesn2 Mouse)

Tags

GST

Expression

Bacterial

Mutation

none

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
pEB2-2ndVal mKate2

Plasmid

#104030

Purpose

Plasmid encoding 2ndVal mKate2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mKate2

Use

Low copy

Expression

Bacterial

Mutation

mKate2 with valine inserted in 2nd position

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pET28-MRPP1

Plasmid

#67862

Purpose

bacterial expression of TRMT10C, full coding sequence (mitoch. form) + N-term. His tag (thrombin site)

Depositor

Walter Rossmanith

Article

Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

Insert

MRPP1 (TRMT10C Human)

Tags

His

Expression

Bacterial

Mutation

mitoch. form (contains aa 40-403)

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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BRD1

Plasmid

#39095

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

BRD1 (BRD1 Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pKM342

Plasmid

#71486

Purpose

Contains loxP-hyg-loxP cassette for recombineering; HygR, AmpR.

Depositor

Kenan Murphy

Article

Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

Insert

loxP-hyg-loxP cassette

Use

Source of loxp-hyg-loxp for pcr amplification.

Expression

Bacterial

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-GST-AGO4

Plasmid

#24319

Depositor

Carl Novina

Article

Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1

Insert

AGO4 (AGO4 Human)

Expression

Bacterial

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only
pKan cpRBP-FL P53

Plasmid

#174504

Purpose

Express Full-length P53 in E.coli with cleavable expression/purification tag (ribose binding protein circular permutant)

Depositor

Stewart Loh

Article

Ha et al Bio Protoc. 2021 Oct 20;11(20):e4188. do

Insert

P53 gene (TP53 Human)

Tags

20 amino acid linker containing HRV3C protease si…

Expression

Bacterial

Promoter

T7

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pTD-C_eYFPTwinStrep

Plasmid

#45942

Purpose

expresses E.coli codon optimized EYFP-Twin-Strep fusion protein

Depositor

Thorben Dammeyer

Article

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Insert

synthetic eYFP (codon usage adapted to E.coli K12)

Tags

Twin-Strep-tag

Expression

Bacterial

Promoter

lacIq/Ptrc

Available Since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pSUMO His6 SUMO PYL1 (36-211)

Plasmid

#177843

Purpose

Bacterial Expression of PYL1

Depositor

Karsten Melcher

Article

Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

Insert

PYR1-like 1 (PYL1 Mustard Weed)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pEE060

Plasmid

#176817

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

611

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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GB1-ALIX(P801G)

Plasmid

#180025

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(1-868, P801G). P801G mutation facilitates expression in E. coli.

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

GB1-His6-TEV-ALIX(1-868, P801G) (PDCD6IP Human)

Tags

GB1-His6-TEV

Expression

Bacterial

Mutation

P801G

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEB2-DsRed-Express

Plasmid

#103995

Purpose

Plasmid encoding DsRed-Express

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

DsRed-Express

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (R2A, K5E, N6D, T21S,…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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