32,457 results
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Plasmid#140631PurposeExpresses ShCAST under the T7 promoter and an empty sgRNADepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA targeting IS1 (Escherichia coli)
UseCRISPR; TransposonExpressionBacterialAvailable SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MinC
Plasmid#133628PurposeExpression vector for the Escherichia coli MinC with an N-terminal fusion of a His-tag for purification.DepositorAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-his-SUMO-RNF168ring (1-189)
Plasmid#63570PurposeExpression of human RNF168 ring domain (residues 1-189) tagged with His-SUMODepositorInsertRNF168 (RNF168 Human)
Tags6xHis and SUMO3ExpressionBacterialMutationRing domain only (residues 1-189)PromoterT7Available SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TEV-U1AF37MF77M
Plasmid#168267PurposeExpresses human dmU1A with the F37M/F77M double mutantDepositorInsertU1A RRM1 crystallization module (SNRPA Human)
Tags6xHis tag N-terminus, TEV protease cleavage siteExpressionBacterialMutationchanged F37M and F77MPromoterT7 promotorAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMGA-ptet-sfGFP
Plasmid#139935PurposeaTc-inducible sfGFP expression on M. magneticum/E. coli shuttle vectorDepositorInsertTetR-Ptet-sfGFP
ExpressionBacterialAvailable SinceJuly 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET11_Ec_ORF209
Plasmid#69029PurposeExpresses E.coli ORF209/ADPRase in bacterial cellsDepositorInsertORF209/ADPRase
Tags-ExpressionBacterialAvailable SinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
CDC42
Plasmid#55693Purposeexpression of mouse CDC42DepositorInsertCDC42
ExpressionBacterialPromoterlacAvailable SinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
TrpLE-M-nMSP1E3D1
Plasmid#179949PurposeTagless membrane scaffold protein (MSP) for formation of nanodiscs.DepositorInsertTrpLE-M-nMSP1E3D1
TagsTrpLEExpressionBacterialMutationNative Trp residues have been mutated to Phe resi…PromoterT7Available SinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKB222
Plasmid#170012PurposeEncodes LacI-controlled expression of surface-displayed LL-37 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
LL-37
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
CLK2
Plasmid#39063PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSETB-ro2GFP
Plasmid#82366PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertro2GFP
Tags6xHISExpressionBacterialMutationC48S/S65T/Q80R/S147C/Q204CPromoterT7Available SinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046733
Plasmid#145665PurposeBacterial Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
4QQD
Plasmid#61947PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertUHRF1:SDC130-H02:C39674 (UHRF1 Human)
Tags6xHis and MHHHHHHSSGRENLYFQGExpressionBacterialMutationSee commentsPromoterT7Available SinceMarch 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTriEx-mCherry-LOVSD
Plasmid#81033PurposeMammalian expression of the super-dark state of LOV2DepositorInsertLOVSD
TagsmCherryExpressionBacterial, Insect, and Mamm…MutationC450A, L514K, G528A, L531E, N538EAvailable SinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
p_SNIPR_sensor_LacZ
Plasmid#139464PurposeT7 RNAP-driven expression of SNIPR RNA with a LacZ reporterDepositorInsertSNIPR_lacZ
ExpressionBacterialAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21a.Tat(48-57)-I-SceI
Plasmid#165041PurposeExpression of Tat(48-57) conjugated I-SceI for protein purification (6XHis tag)DepositorInsertI-SceI
Tags6XHis and Tat(48-57)ExpressionBacterialAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_MANF-Halo
Plasmid#175341PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHBJT001
Plasmid#225152PurposeHigh copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialAvailable SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_13
Plasmid#194674PurposeProtein production of the split HaloTag based calcium recorder Caprola_13 in bacteriaDepositorInsertCaprola_13
TagsHis-tag and Strep-tagExpressionBacterialPromoterT7Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDL17_Cas9-His Δcys M1C
Plasmid#206251PurposeBacterial expression of SpCas9 Δcys M1C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationM1C, C80S, C574SPromoterT7Available SinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only