32,156 results
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Plasmid#180313Purposebacterial co-expression of human SEPT2 fused to superfolder Cherry2 and of human SEPT6DepositorTagsHis6-TEV and sfCherry2ExpressionBacterialAvailable SinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only
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pHH0103_HCLS1-1/1
Plasmid#91437PurposeProtein expression and purification of human SH3 domain construct HCLS1-1/1DepositorInsertHCLS1-1/1 (HCLS1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNDGG004
Plasmid#231317PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-pBBR1-Tc-par-ELT4; GG assembly from pOGG011, pOGG012, pOGG014, pOGG042, and pOGG004, TcRDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG051
Plasmid#231320PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-pBBR1-Sp-par-ELT4; GG assembly from pOGG004, pNDGG002, pOGG011, pOGG012, and pOGG014, SpRDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG053
Plasmid#231322PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-pBBR1-Km-par-ELT4; GG assembly from pOGG004, pNDGG001, pOGG011, pOGG012, and pOGG014, NmR/KmRDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMBA328
Plasmid#214748PurposeMedium copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)DepositorInsertBBa_J23119-RBS(22821)-mcherry
ExpressionBacterialAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBA333
Plasmid#214745PurposeMedium copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)DepositorInsertBBa_J23119-RiboJ-RBS(21992)-gfpmut3
ExpressionBacterialAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-ISG15 C78SN13Y
Plasmid#165106Purposebacterial expressio of a GST fusion of Human ISG15 C78SN13YDepositorInsertISG15 (ISG15 Human)
TagsGST from plasmid.ExpressionBacterialMutationC78SN13Y; The sequence of ISG15-C78S/N13Y contai…Available SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28Teb1
Plasmid#28173DepositorInsertTeb1
Tags6xhistidineExpressionBacterialAvailable SinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4092694
Plasmid#153907PurposeBacterial expression plasmid for fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stopDepositorInsertfragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
ExpressionBacterialMutationwtPromoterPromoter | t7 consensus from NEB, G is +1Available SinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
4LEC
Plasmid#60091PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMETTL21A:JMC072-B07:C227766 (METTL21A Human)
Tags6xHisExpressionBacterialMutationSee commentsPromoterT7Available SinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
MYST1
Plasmid#25180PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 9, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTn7xKS-sfGFP
Plasmid#117394PurposeTn7 tagging vector pTn7xKS with sfGFP expression scaffoldDepositorInsertsfGFP
UseSynthetic BiologyExpressionBacterialPromoterPtacAvailable SinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Zeo-ecfp
Plasmid#65038PurposeZeoR mini-Tn7 vector, mobilizable, for CFP tagging bacteriaDepositorInsertecfp
ExpressionBacterialAvailable SinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNIC-CH10-GG
Plasmid#215807PurposeCloning of target gene with C terminal non cleavable His10 tagDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
gNS2B47NS3 S135A
Plasmid#155317PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker S135A mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
TagsHis tagExpressionBacterialMutationS135APromoterM13Available SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEND-193_Cacnes_HS
Plasmid#225622PurposeC. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS06745)+RBS_1+HspR(PPA_RS10230)DepositorInsertHspR
UseSynthetic BiologyTagspFAST-6XHisExpressionBacterialAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-229_Cacnes_EforCP
Plasmid#225615PurposeReplicative vector for red fluorescent reporter protein eForCP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+eForCPDepositorInsertEforCP
UseSynthetic BiologyTags6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
BPTI-[5,55]Gly14Ile38
Plasmid#195059PurposeThermal stability of BPTIDepositorInsertbovine pancreatic trypsin inhibitor
TagsHis tag and trpΔLE leader sequencesExpressionBacterialMutationChanged Alanine residues at position of 14 and 38…PromoterT7 promotorAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0835:Dendra
Plasmid#174923PurposeC-term fluorescent fusionDepositorInsertMSMEG_0835
UseIntegrating mycobacterial expression vector attl5…TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialPromoterpmycTetOAvailable SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only