30,808 results

1. SpIMPDH

   Plasmid

   #206980

   Purpose

   Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.

   Depositor

   Vern Schramm

   Article

   Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210

   Insert

   SpIMPDH (guaB Streptococcus pyogenes)
   

   Tags

   His tag (with TEV cleavage site)

   Expression

   Bacterial

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. T7RNAP Cyslight A188C

   Plasmid

   #205083

   Purpose

   T7RNAP protein expression

   Depositor

   Smita Patel

   Insert

   T7RNAP Cyslight A188C (T7p07 Bacteriophage T7)
   

   Expression

   Bacterial

   Mutation

   C125S, C347S, C510S, C515S, C530S, C723S N88C

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-S9

   Plasmid

   #132948

   Purpose

   Expresses N-terminal 6xHis-tagged S9 ribosomal protein in E.coli

   Depositor

   James Williamson

   Article

   Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

   Insert

   30S ribosomal subunit protein S9 (rpsI E.coli)
   

   Tags

   N-terminal 6xHis-tag

   Expression

   Bacterial

   Mutation

   E52C

   Promoter

   T7

   Available Since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-DEST42-ARF6-V5-His6

   Plasmid

   #67415

   Purpose

   Expression of hArf6 with C-term V5 and His6 tag in E. coli

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARF6 (ARF6 Human)
   

   Tags

   His and V5

   Expression

   Bacterial

   Promoter

   T7/lacO

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pET-UbcH10-wt

   Plasmid

   #8503

   Depositor

   Joan Ruderman

   Article

   Bastians et al Mol Biol Cell 1999 Nov;10(11):3927-41.

   Insert

   UbcH10 (UBE2C Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   June 1, 2005

   Availability

   Academic Institutions and Nonprofits only

6. pGEX2TK-Skm1-PH

   Plasmid

   #71371

   Purpose

   bacterial expression of PH domain for phosphoinositide binding assay

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   Skm1-PH
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa1-132

   Promoter

   tac

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

7. BRPF1B-c001

   Plasmid

   #53620

   Purpose

   bacterial expression plasmid

   Depositor

   Nicola Burgess-Brown

   Insert

   BRPF1B
   

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   Bacterial expression for structure determination;…

   Promoter

   T7

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pRF-LmrA

   Plasmid

   #49364

   Depositor

   Christopher Voigt

   Article

   Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

   Insert

   LmrA repressor
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. PclpB GFP pZa

   Plasmid

   #170074

   Purpose

   GFP expression under the control of E. coli clpB promoter

   Depositor

   Urartu Seker

   Article

   Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

   Insert

   Green fluorescent protein
   

   Expression

   Bacterial

   Promoter

   PclpB

   Available Since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAV105

    Plasmid

    #63195

    Purpose

    pRS405 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEB2-mCherry-L

    Plasmid

    #104002

    Purpose

    Plasmid encoding mCherry-L

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mCherry-L
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to DsRed (MSKGEE DNLA IIKEF… V…

    Promoter

    proC

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBV58

    Plasmid

    #204603

    Purpose

    ERG11-3X Minidegron-5X FLAG-HIS3MX6

    Depositor

    Scott Moye-Rowley

    Insert

    Candida glabrata ERG11 (ERG11 )
    

    Tags

    auxin-inducible degron

    Expression

    Bacterial

    Available Since

    July 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC18T-mini-Tn7T-hph

    Plasmid

    #218366

    Purpose

    Empty backbone for Tn7-site insertion

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMApra-Ptol

    Plasmid

    #218359

    Purpose

    Empty vector with XylS-Pm expression system

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC18T-mini-Tn7T-hph-Ptol

    Plasmid

    #218369

    Purpose

    Empty backbone for Tn7-site insertion with XylS-Pm expression system

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pExp-RBD-CHis

    Plasmid

    #195002

    Purpose

    Production of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Article

    Fischer et al bioRxiv 2023.01.10.523494

    Insert

    RBD (S SARS-CoV-2)
    

    Tags

    His8

    Expression

    Bacterial

    Promoter

    T7lac

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. HOXA7 (murine) His-tag pET

    Plasmid

    #8491

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HOXA7 (Hoxa7 Mouse)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Fusion sequence is: ggatccATG; makes protein by …

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pQE80L TriCatcher-RGDSP

    Plasmid

    #112631

    Purpose

    Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site and a C-terminal SnoopCatcher

    Depositor

    Mark Howarth

    Article

    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

    Insert

    TriCatcher-RGDSP
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    central RGDSP integrin-binding sequence

    Promoter

    T5

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEB1-mEmerald

    Plasmid

    #103982

    Purpose

    Plasmid encoding mEmerald

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mEmerald
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F64L, S65T, …

    Promoter

    proC

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. EPHB1_HUMAN_D0

    Plasmid

    #79694

    Purpose

    This plasmid encodes the kinase domain of EPHB1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    EPHB1 (EPHB1 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only