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pET28_6xHis-PARP1-Y986H

Plasmid

#174801

Purpose

Bacterial expression of a PARP1 mutant that produces highly branched but short PAR chains overall

Depositor

Joan Conaway

Publication

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Y986H (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Y986H, V762A

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-Y986S

Plasmid

#174802

Purpose

Bacterial expression of a PARP1 mutant that produces mainly short PAR oligomers

Depositor

Joan Conaway

Publication

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Y986S (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Y986H, V762A

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-BRCT

Plasmid

#174807

Purpose

Bacterial expression of isolated PARP1 BRCT domain

Depositor

Joan Conaway

Publication

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-BRCT (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-WGR

Plasmid

#174808

Purpose

Bacterial expression of isolated PARP1 WGR domain

Depositor

Joan Conaway

Publication

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-WGR (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pESUMO-hRORg2

Plasmid

#170329

Purpose

Expresses RORg isoform 2 with N-terminal HisSUMO tag in bacteria

Depositor

Patrick Griffin

Publication

Strutzenberg et al J Mol Biol. 2021 Sep 20;433(22):167258.

Insert

RORG isoform 2 (RORC Human)

Tags

HisSUMO

Expression

Bacterial

Mutation

codon optimized

Promoter

T7

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pESUMO-hRORg1

Plasmid

#170330

Purpose

Expresses RORg isoform 1 with N-terminal HisSUMO tag in bacteria

Depositor

Patrick Griffin

Publication

Strutzenberg et al J Mol Biol. 2021 Sep 20;433(22):167258.

Insert

RORG isoform 1 (RORC Human)

Tags

HisSUMO

Expression

Bacterial

Promoter

T7

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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bdSUMO-HA2-GFP-HiBit

Plasmid

#171017

Purpose

For bacterial expression of bdSUMO tagged muGFP with HA2 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Publication

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-HA2-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pED14xS-1

Plasmid

#134800

Purpose

IPTG inducible expression of S-TAGA qtRNA

Depositor

Ahmed Badran

Publication

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

S-TAGA qtRNA

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pED14xS-WT

Plasmid

#134804

Purpose

IPTG inducible expression of WT S tRNA scaffold tRNA

Depositor

Ahmed Badran

Publication

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

Wildtype S tRNA (decodes TCG)

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pSaPIPLC His

Plasmid

#173810

Purpose

Expression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cells

Depositor

Anne Gershenson, Mary F Roberts

Publication

Goldstein et al Biochemistry. 2012 Mar 27;51(12):2579-87

Insert

Staphylococcus aureus plc

Tags

6XHis

Expression

Bacterial

Mutation

none

Promoter

T7

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pWSK129-stm2585

Plasmid

#174401

Purpose

S. Typhimurium stm2585 (sarA/steE) flanked with endogenous regulatory sequences (786bp on 5' and 28bp on 3' end) for bacterial expression.

Depositor

Dennis Ko

Publication

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Expression

Bacterial

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21-pelB-LaG-17

Plasmid

#172747

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-17

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1alpha-mClover3-bGHpA

Plasmid

#172286

Purpose

Constitutive expression of mClover3

Depositor

Patrik Verstreken

Insert

mClover3

Expression

Bacterial

Promoter

EF1alpha

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-29

Plasmid

#172753

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-29

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-10

Plasmid

#172743

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-10; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-10

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-1

Plasmid

#172767

Purpose

Bacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-1

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-6

Plasmid

#172741

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-6; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-6

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-30

Plasmid

#172754

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-30

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMAL630

Plasmid

#167836

Purpose

Encodes OptoLAC3 circuit

Depositor

Jose Avalos

Publication

Lalwani et al Nat Chem Biol. 2021 Jan;17(1):71-79. doi

Insert

lacI

Use

Synthetic Biology

Tags

ssrA (AAV)

Expression

Bacterial

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-42

Plasmid

#172758

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-42; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-42

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge