33,532 results

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pHH0103_THRB_Hormone-recep

Plasmid

#109819

Purpose

Protein expression and purification of THRB_Hormone-recep

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

THRB_Hormone-recep (THRB Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_RPA2_RPA-C

Plasmid

#109795

Purpose

Protein expression and purification of RPA2_RPA-C

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RPA2_RPA-C (RPA2 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_AKT1_Pkinase

Plasmid

#109775

Purpose

Protein expression and purification of AKT1_Pkinase

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

AKT1_Pkinase (AKT1 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2_Psyn-sgRNA500

Plasmid

#149613

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pTSSX-STC

Plasmid

#115668

Purpose

E.coli expression plasmid of small tetraheme cytochrome (STC) from Shewanella oneidensis MR-1 with cleavable Strep tag

Depositor

David Kramer

Publication

Huang et al J Am Chem Soc. 2020 Jun 10;142(23):10459

Insert

small tetraheme cytochrome c

Tags

Signal peptide (aa1-27)-Strep-II tag- Factor Xa c…

Expression

Bacterial

Promoter

tac

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP2832

Plasmid

#160672

Purpose

Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

Depositor

Jason Peters

Publication

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

gmc6 (gfp) sgRNA

Expression

Bacterial

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP2820

Plasmid

#160669

Purpose

Mobile CRISPRi sfGFP 'test', empty sgRNA

Depositor

Jason Peters

Publication

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

empty sgRNA (BsaI)

Expression

Bacterial

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP2782

Plasmid

#160668

Purpose

Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

Depositor

Jason Peters

Publication

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Type

Empty backbone

Expression

Bacterial

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pXZ13lacZalpha

Plasmid

#160229

Purpose

places LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal plates

Depositor

Ian Duncan

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

lac

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X71-527: His6-tev-Hs.CALM1(1-80)

Plasmid

#159694

Purpose

E. coli protein expression of His6-tev-Hs.CALM1(1-80)

Depositor

Dominic Esposito

Publication

Agamasu et al Biophys J. 2019 Mar 19;116(6):1049-1063.

Insert

His6-tev-Hs.CALM1(1-80) (CALM1 Human)

Tags

His6-tev

Expression

Bacterial

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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R717-X45-566: His6-MBP-tev-Hs.RAF1(136-188)

Plasmid

#159698

Purpose

E. coli protein expression of His6-MBP-tev-Hs.RAF1(136-188)

Depositor

Dominic Esposito

Publication

Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

Insert

His6-MBP-tev-Hs.RAF1(136-188) (RAF1 Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pRibo-Tt

Plasmid

#154124

Purpose

Expresses wt Ribo-T rRNA and a set of tRNAs in bacterial cells

Depositor

Alexander Mankin

Publication

Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

Inserts

engineered bacterial rRNAs operon under control of pL promotor
bacterial tRNAs (Asp, Trp, Ile, Ala, Glu) operon under control of Ptac promoter

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptac and pL

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pKP325

Plasmid

#159596

Purpose

Express E.coli TonB protein

Depositor

Kathleen Postle

Publication

Larsen et al Mol Microbiol. 1999 Mar;31(6):1809-24. d

Insert

tonB

Expression

Bacterial

Mutation

none

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X54-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D

Plasmid

#159562

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D

Depositor

Dominic Esposito

Publication

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

G12D

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X28-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D

Plasmid

#159548

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D

Depositor

Dominic Esposito

Publication

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D (KRAS Human)

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

G12D

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X21-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12D

Plasmid

#159569

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12D

Depositor

Dominic Esposito

Publication

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12D (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

G12D

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X11-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G13D

Plasmid

#159543

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G13D

Depositor

Dominic Esposito

Publication

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-169) G13D (KRAS Human)

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

G13D

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X10-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12V

Plasmid

#159542

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12V

Depositor

Dominic Esposito

Publication

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-169) G12V (KRAS Human)

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

G12V

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pZmUbi-5'UTR

Plasmid

#154057

Purpose

Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Wheat construct)

Depositor

Cristobal Uauy

Publication

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

Promoter and 5' UTR Ubiquitin (Zea mays)

Use

Synthetic Biology

Expression

Bacterial

Promoter

N/A

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pKL12-GCaMP6f-pHuji bb118

Plasmid

#158981

Purpose

Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to pHuji fluorophores in E. coli.

Depositor

Joel Kralj

Publication

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

GCaMP6f-pHuji

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#100833 a…

Promoter

Bba_J23118 (sigma 70)

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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