30,808 results

1. pGEX6P2-MDMX (1-120)

   Plasmid

   #62065

   Purpose

   Expression of human MDM2 (1-120) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

   Insert

   MDM4 (MDM4 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains residues 1-120

   Promoter

   tac

   Available Since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pVC-Ds-Pax3a_enh5-E1b:eGFP-Ds

   Plasmid

   #119059

   Purpose

   Fluorescent vector with zebrafish pax3a enhancer 5 (strong activity)

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   pax3a_enh5-E1b:eGFP
   

   Expression

   Bacterial

   Promoter

   E1b minimal promoter

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGSTag Flag Jnk1a1

   Plasmid

   #15743

   Depositor

   Roger Davis

   Article

   Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

   Insert

   Jnk1a1 (MAPK8 Human)
   

   Tags

   Flag

   Expression

   Bacterial

   Available Since

   March 6, 2008

   Availability

   Academic Institutions and Nonprofits only

4. ALFY-FYVE 1G

   Plasmid

   #197045

   Purpose

   Expression plasmid for the FYVE domain from ALFY for recombinant expression in E. coli.

   Depositor

   Michael Ragusa

   Article

   Reinhart et al Traffic. 2021 Jan;22(1-2):23-37. doi: 10

   Insert

   ALFY (WDFY3 Human)
   

   Tags

   6xHis-GST-TEV

   Expression

   Bacterial

   Available Since

   Dec. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pXD71Cas10.1RT1-Pct5.1-crRNA(dadR)-RT(ΔdadR)

   Plasmid

   #191635

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer) dadR-deleting repair template, used for dadR deletion

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   CymR
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pXD70Tet(DadR)-DadR

   Plasmid

   #191636

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR complementation

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   DadR
   

   Expression

   Bacterial

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ZFYVE9C-c009

   Plasmid

   #53618

   Purpose

   bacterial expression plasmid

   Depositor

   Nicola Burgess-Brown

   Insert

   ZFYVE9C
   

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   Bacterial expression for structure determination;…

   Promoter

   T7

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pHH0103_SH3GLB1-1/1

   Plasmid

   #91346

   Purpose

   Protein expression and purification of human SH3 domain construct SH3GLB1-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SH3GLB1-1/1 (SH3GLB1 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pACEC3GH (Apr)

   Plasmid

   #110085

   Purpose

   Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Tags

   3C cleavage site - GFP - His10 Tag

   Expression

   Bacterial

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMINTC3GH (Kan)

    Plasmid

    #110076

    Purpose

    Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Tags

    3C cleavage site - GFP - His10 Tag

    Expression

    Bacterial

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pQLinkHD_EBFP2-LSSmOrange

    Plasmid

    #118856

    Purpose

    fluorescent protein expression with an N-terminal His-tag

    Depositor

    Marc Birtwistle

    Article

    Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

    Insert

    EBFP2-LSSmOrange
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    EBFP1.2-I128V/V150I/ D155V/V224R and for LSSmOra…

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRGM1

    Plasmid

    #48789

    Purpose

    HA-I-SceI expression plasmid, episomal

    Depositor

    Michael Glickman

    Article

    Gupta et al Mol Microbiol. 2011 Jan;79(2):316-30. do

    Insert

    I-SceI
    

    Tags

    HA tag

    Expression

    Bacterial

    Promoter

    MOP

    Available Since

    Jan. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTHSSd_27

    Plasmid

    #59958

    Purpose

    PTac null fragment (sigma CGG Y639A) expression

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

    Insert

    null fragment
    

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    PTac

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Z-AIMP1-1

    Plasmid

    #166560

    Purpose

    scFv of a human scaffold targeting Aminoacyl tRNA synthase complex-interacting multifunctional protein 1. Antigen coverage aa 147-312 of 312

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21b-LOV-ipaA

    Plasmid

    #40236

    Depositor

    Brian Kuhlman

    Article

    Lungu et al Chem Biol. 2012 Apr 20;19(4):507-17.

    Insert

    LOV-ipaA
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Jα helix region hybridized (AsLOV2 aa 537-546/ipa…

    Promoter

    T7

    Available Since

    Sept. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pHisIHFαIHFβ

    Plasmid

    #149384

    Purpose

    Expression of P. aeruginosa PA14, 6xHis-HRV3C-IHFalpha and IHFbeta

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Inserts

    Pseudomonas aeruginosa PA14 6xHis-tagged IHFalpha
    Pseudomonas aeruginosa PA14 IHFbeta
    

    Tags

    6xHis tag and HRV3C cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pStrepIHFb

    Plasmid

    #149385

    Purpose

    Expression of P. aeruginosa PA14, StrepII-HRV3C-IHFbeta

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 StrepII-tagged IHFbeta
    

    Tags

    HRV3C cleavage site and StrepII-tag with

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHH0103 FNBP3 WW domain #1

    Plasmid

    #104233

    Purpose

    Bacterial expression of WW domain #1 from FNBP3

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    FNBP3 WW domain #1 (PRPF40A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    March 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMBP-LbCas12a H759A

    Plasmid

    #113518

    Purpose

    Expresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processing

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    LbCas12a
    

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    H759A

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HOXA10 (human) HIS-tag pET

    Plasmid

    #8516

    Depositor

    Corey Largman

    Article

    Lowney et al Nucleic Acids Res 1991 Jun 25;19(12):344

    Insert

    HOXA10 (HOXA10 Human)
    

    Expression

    Bacterial

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only